25 documents found, page 1 of 3
Physical Interactions With Bacteria and Protozoan Parasites Establish the Scave...
Cardoso, MS; Santos, RF; Almeida, S; Sá, M; Perez-Cabezas, B; Oliveira, L; Tavares, J; Carmo, AMSince the pioneering discoveries, by the Nobel laureates Jules Hoffmann and Bruce Beutler, that Toll and Toll-like receptors can sense pathogenic microorganisms and initiate, in vertebrates and invertebrates, innate immune responses against microbial infections, many other families of pattern recognition receptors (PRRs) have been described. One of such receptor clusters is composed by, if not all, at least sev...
Urgent-Start Peritoneal Dialysis – a viable option? A case report and literatur...
Tavares, J; Silva, F; Lima, A; Carvalho, MJ; Cabrita, A; Rodrigues, ABackground: Many patients with end -stage renal disease start renal replacement therapy in an unplanned manner. The vast majority initiate hemodialysis by a central venous catheter, since its use is more widespread and available. This technique is associated with a high risk of infection and damage of the vascular patrimony associated with the use of central veins. Urgent -start peritoneal dialysis comes as an ...
Targeted Next-Generation Sequencing Study in Familial ALS-FTD Portuguese Patien...
Gromicho, M; Coutinho, AM; Pronto-Laborinho, AC; Raposeiro, R; Tavares, J; Antunes, D; de Carvalho, MAmyotrophic lateral sclerosis (ALS) is a neurodegenerative disease with clinical and etiological heterogeneity and a complex genetic contribution. Clinical, neuropathological, and genetic evidence revealed that ALS and frontotemporal dementia (FTD) are in part of a single disease continuum. Genetic causes have been identified in sporadic (SALS) and familial patients (FALS) and the recurrent genetic factor under...
Global Ultrasound Check for the Critically lll (GUCCI)-a new systematized proto...
Tavares, J; Ivo, R; Gonzalez, F; Lamas, T; Mendes, JJUltrasound technology is an essential tool in the management of critically ill patients. Point-of-care ultrasonography (POCUS) enables data collection from different anatomic areas to achieve the most probable diagnosis and administer the right therapy at the right time. Despite the increasing utilization of POCUS, there is still a lack of standards to establish how to use different bedside ultrasound protocols...
Murine infection with bioluminescent Leishmania infantum axenic amastigotes app...
Costa, DM; Cecílio, P; Santarém, N; Cordeiro-da-Silva, A; Tavares, JLeishmaniasis is an important vector-borne neglected tropical disease caused by Leishmania parasites. Current anti-Leishmania chemotherapy is unsatisfactory, justifying the continued search for alternative treatment options. Herein, we demonstrate that luciferase-expressing Leishmania infantum axenic amastigotes, unlike promastigotes, are highly infectious to BALB/c mice and thus generate a robust bioluminescen...
Inhibitors of Trypanosoma cruzi Sir2 related protein 1 as potential drugs again...
Gaspar, L; Coron, R; KongThoo Lin, P; Costa, DM; Perez-Cabezas, B; Tavares, J; Roura-Ferrer, M; Ramos, I; Ronin, C; Major, L; Ciesielski, FChagas disease remains one of the most neglected diseases in the world despite being the most important parasitic disease in Latin America. The characteristic chronic manifestation of chagasic cardiomyopathy is the region’s leading cause of heart-related illness, causing significant mortality and morbidity. Due to the limited available therapeutic options, new drugs are urgently needed to control the disease. S...
Potential drug targets in the pentose phosphate pathway of trypanosomatids
Loureiro, I; Faria, J; Santarem, N; Smith, TK; Tavares, J; Cordeiro-da-Silva, AThe trypanosomatids, Trypanosoma brucei, Trypanosoma cruzi and Leishmania spp, are causative agents of important human diseases such African sleeping sickness, Chagas' disease and Leishmaniasis, respectively. The high impact of these diseases on human health and economy worldwide, the unsatisfactory available chemotherapeutic options and the absence of human effective vaccines, strongly justifies the search for...
The crystal structure of the Leishmania infantum Silent Information Regulator 2...
Ronin, C; Costa, DM; Tavares, J; Faria, J; Ciesielski, F; Ciapetti, P; Smith, TK; MacDougall, J; Cordeiro-da-Silva, A; Pemberton, IThe de novo crystal structure of the Leishmania infantum Silent Information Regulator 2 related protein 1 (LiSir2rp1) has been solved at 1.99Å in complex with an acetyl-lysine peptide substrate. The structure is broadly commensurate with Hst2/SIRT2 proteins of yeast and human origin, reproducing many of the structural features common to these sirtuin deacetylases, including the characteristic small zinc-binding...
TRSP is dispensable for the Plasmodium pre-erythrocytic phase
Costa, DM; Sá, M; Teixeira, AR; Loureiro, I; Thouvenot, C; Golba, S; Amino, R; Tavares, JPlasmodium sporozoites deposited in the skin following a mosquito bite must migrate and invade blood vessels to complete their development in the liver. Once in the bloodstream, sporozoites arrest in the liver sinusoids, but the molecular determinants that mediate this specific homing are not yet genetically defined. Here we investigate the involvement of the thrombospondin-related sporozoite protein (TRSP) in ...
Errors in protein synthesis increase the level of saturated fatty acids and aff...
Araújo, A; Melo, T; Maciel, E; Pereira, C; Morais, C; Santinha, D; Tavares, J; Oliveira, H; Jurado, A; Costa, V; Domingues, P; Domingues, M; Santos, MThe occurrence of protein synthesis errors (mistranslation) above the typical mean mistranslation level of 10−4 is mostly deleterious to yeast, zebrafish and mammal cells. Previous yeast studies have shown that mistranslation affects fitness and deregulates genes related to lipid metabolism, but there is no experimental proof that such errors alter yeast lipid profiles. We engineered yeast strains to misincorpo...