2 documents found, page 1 of 1
Estimating pentose phosphate pathway activity from the analysis of hepatic glyc...
Belew, Getachew D.; Di Nunzio, Giada; Tavares, Ludgero; Silva, Joao G.; Torres, Alejandra N.; Jones, John GriffithThe pentose phosphate pathway (PPP) is an important component of hepatic intermediary metabolism. Jin et al developed an elegant 13 C-NMR method for measuring hepatic PPP flux by quantifying the distribution of glucose 13 C-isotopomers formed from [U-13 C]glycerol. We demonstrate that this approach can be extended to exogenous [U-13 C]fructose and [U-13 C]glucose precursors by 13 C-NMR analysis of glycogen.; Re...
Determining the contribution of a high-fructose corn syrup formulation to hepat...
DiNunzio, Giada; Belew, Getachew D.; Torres, Alejandra N.; Silva, Joao Gabriel; Silva, Luís P.; Barosa, Cristina; Tavares, Ludgero; Jones, John G.Excessive sugar intake including high-fructose corn syrup (HFCS) is implicated in the rise of obesity, insulin resistance and non-alcoholic fatty liver disease. Liver glycogen synthesis is influenced by both fructose and insulin signaling. Therefore, the effect of HFCS on hepatic glycogenesis was evaluated in mice feeding ad-libitum. Using deuterated water: the fraction of glycogen derived from triose-P sources...