5 documents found, page 1 of 1
Manufacture of bioactive peptide-rich concentrates from whey: characterization ...
Tavares, Tânia G.; Amorim, Manuela; Gomes, David; Pintado, Manuela E.; Pereira, Carlos D.; Malcata, F. XavierThis work was focused on the manufacture, at pilot scale, of cow whey protein and peptide concentrates, using selective filtration techniques—associated with hydrolysis brought about by proteolytic enzymes from Cynara cardunculus aqueous extracts, using as (optimal) conditions an enzyme/substrate ratio of 1.6% v/v, a pH of 5.2, a temperature of 55 C and an incubation time of 7 h. The profiles of proteins and pe...
The Portuguese Paradox: why do some inhabitants of Portugal appear to live so l...
Tavares, Tânia G.; Malcata, F. XavierThe remarkable longevity of people in specific regions of Portugal, whose diets are based on whey cheese, has puzzled researchers for quite some time. Our data indicate that several oligopeptides are released from glycomacropeptide (originated in j-casein) and a-lactalbumin – and one surprisingly from b-lactoglobulin, by plant proteases previously used in cheesemaking. A few of such peptides (e.g. DKVGINYW, KGY...
Influence of native lactic acid bacteria on the microbiological, biochemical an...
Macedo, Angela C.; Tavares, Tânia G.; Malcata, F. XavierCheesemaking from batches of raw ewe’s milk was carried out via inoculation with wild strains of Lactococcus lactis subsp. lactis ESB110019 and Lactobacillus plantarum ESB5004 independently, or combined with each other. Those two strains had been isolated from the native microflora of typical Serra da Estrela cheese. One control batch was processed in parallel without addition of any starter. The evolution in v...
Purification and characterization of an intracellular aminopeptidase from a wil...
Macedo, Angela C.; Tavares, Tânia G.; Malcata, F. XavierAn intracellular hydrolase able to cleave Image -lysine-p-nitroanilide was purified from Lactobacillus plantarum strain ESB5004 via two steps of precipitation with ammonium sulfate (at 30 and 50% (w/v)), followed by hydrophobic interaction and ion-exchange chromatographies. The aminopeptidase was purified up to 11-fold, with a final yield of ca. 1%. Its native molecular weight is ca. 70 kDa, and it is apparentl...
Esterase activities of intracellular extracts of wild strains of lactic acid ba...
Macedo, Angela C.; Tavares, Tânia G.; Malcata, F. XavierLactococcus lactis subsp. lactis strain ESB110019 and Lactobacillus plantarum strain ESB5004, novel strains that were previously isolated from the wild adventitious microflora of certified Serra da Estrela cheeses, were assayed for esterase activity using, as substrates, ortho- and para-nitrophenyl derivatives of fatty acids. Both strains preferentially hydrolyzed short-chain fatty acids; L. lactis ESB110019 ex...