9 documents found, page 1 of 1

Metagenomic sequencing with spiked primer enrichment for viral diagnostics and ...

Metagenomic next-generation sequencing (mNGS), the shotgun sequencing of RNA and DNA from clinical samples, has proved useful for broad-spectrum pathogen detection and the genomic surveillance of viral outbreaks. An additional target enrichment step is generally needed for high-sensitivity pathogen identification in low-titre infections, yet available methods using PCR or capture probes can be limited by high c...

Princípios Gerais de Bacteriologia. Microbiologia Médica – Vol. 1

As bactérias são procariotas que pertencem ao domínio Bacteria e apresentam três formas básicas: esférica, cilíndrica e helicoidal. As células podem associar-se, formando arranjos característicos de alguns géneros bacterianos. A parede celular, além de conferir forma às bactérias, é responsável pela diferenciação entre bactérias Gram-positivo e Gram-negativo. Além disso, é um alvo preferencial para a atuação de...

Inhibition of HIV cell-to-cell fusion by antiretroviral drugs and neutralizing ...

"Inhibition of HIV cell entry by antiretroviral drugs and neutralizing antibodies (NAbs) is typically measured in assays where cell-free virions enter reporter cell lines. However, direct Env-mediated cell-to-cell transmission is a major mechanism of HIV infection that also needs to be targeted. In this work we aimed to determine the ability of anti-HIV compounds in clinical or research use to inhibit HIV media...

Envelope C2-V3-C3-specific antibodies from HIV-1 infected patients from Angola ...

"Development of immunogens that induce broadly neutralizing antibodies (bNAbs) is a major goal in HIV-1 vaccine field. Recently, we found that bNAbs can be elicited in Balb/c mice against HIV-2 by using a prime-boost vaccination strategy combining recombinant Vaccinia virus expressing a truncated form of the SU glycoprotein and a polypeptide comprising the C2, V3 and C3 envelope regions. We want to test the hyp...

Entry inhibitors and Carbosilane dendrimers are potent inhibitors of cell-assoc...

"Pre-exposure prophylaxis with topical microbicides formulated with antiviral agents can prevent HIV-1 acquisition. The ideal microbicide should prevent both cell-free and cell-associated HIV-1 and HIV-2 infections in the vaginal or rectum mucosa. So far, few candidate microbicides have been tested against HIV-2. Previously, we have shown that anionic carbosilane dendrimers 2G-S16 (sulphonate), G2-NF16 (naphthy...

Evaluation of an in-house molecular HIV-1 test to assess mother-to-child HIV-1 ...

"Mother-to-child-transmission (MTCT) rate has decreased sharply in recent years in most of the sub-Saharan Africa, however 220,000 children acquired HIV-1 in 2014. PCR detection of proviral DNA is the most sensitive method for early infant diagnosis (EID) of HIV-1 infection. Commercial kits are available but they have poor sensitivity with divergent non-B subtypes and high costs (≈30€ per test) which limit thei...

Medicines review in the elderly

Memory B-cell depletion is a feature of HIV-2 infection even in the absence of ...

Objective: Memory B-cell loss has long been recognized as an important contributor to HIV immunodeficiency. HIV-2 infection, which is characterized by a slow rate of progression to AIDS and reduced to undetectable viremia, provides a unique model to investigate B-cell disturbances. Design and methods: B-cell subsets were evaluated in 38 HIV-2-infected individuals, along with markers of T-cell activation and ser...

Outbreak of acute respiratory infection among infants in Lisbon, Portugal, caus...

Human adenoviruses (AdVs) typically cause mild illnesses in otherwise healthy hosts. We investigated a pediatric outbreak of acute respiratory infection with fatal outcomes that occurred in Lisbon, Portugal, in 2004.Biological specimens were collected from 83 children attending two nurseries, a kinesiotherapy clinic, and the household of a nanny. Adenovirus infection was confirmed in 48 children by PCR and viru...