5 documents found, page 1 of 1
Microbial interactions among Gardnerella, Prevotella and Fannyhessea prior to i...
Muzny, Christina A.; Elnaggar, Jacob H.; Sousa, Lúcia Filipa Guimarães Vieira; Lima, Ângela Martins; Aaron, Kristal J.; Eastlund, Isaac C.Introduction The aetiology of bacterial vaginosis (BV), a biofilm-associated vaginal infection, remains unknown. Epidemiologic data suggest that it is sexually transmitted. BV is characterised by loss of lactic acid-producing lactobacilli and an increase in facultative and strict anaerobic bacteria. Gardnerella spp are present in 95\%\textendash100\% of cases; Gardnerella vaginalis has been found to be more vir...
The role of prevotella species in female genital tract infections
George, Sheridan D.; Van Gerwen, Olivia T.; Dong, Chaoling; Sousa, Lúcia Filipa Guimarães Vieira; Cerca, Nuno; Elnaggar, Jacob H.Female genital tract infections (FGTIs) include vaginal infections (e.g., bacterial vaginosis [BV]), endometritis, pelvic inflammatory disease [PID], and chorioamnionitis [amniotic fluid infection]. They commonly occur in women of reproductive age and are strongly associated with multiple adverse health outcomes including increased risk of HIV/sexually transmitted infection acquisition and transmission, inferti...
A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accur...
Elnaggar, Jacob H.; Ardizzone, Caleb M.; Cerca, Nuno; Toh, Evelyn; Laniewski, Pawel; Lillis, Rebecca A.; Herbst-Kralovetz, Melissa M.; Quayle, Alison J.Bacterial vaginosis (BV) is the most common vaginal dysbiosis. In this condition, a polymicrobial biofilm develops on vaginal epithelial cells. Accurately quantifying the bacterial burden of the BV biofilm is necessary to further our understanding of BV pathogenesis. Historically, the standard for calculating total bacterial burden of the BV biofilm has been based on quantifying Escherichia coli 16S rRNA gene c...
DNA extraction leads to bias in bacterial quantification by qPCR
Lima, Ângela Martins; França, Ângela Maria Oliveira Sousa; Muzny, Christina A.; Taylor, Christopher M.; Cerca, NunoQuantitative PCR (qPCR) has become a widely used technique for bacterial quantification. The affordability, ease of experimental design, reproducibility, and robustness of qPCR experiments contribute to its success. The establishment of guidelines for minimum information for publication of qPCR experiments, now more than 10 years ago, aimed to mitigate the publication of contradictory data. Unfortunately, there...
An updated conceptual model on the pathogenesis of bacterial vaginosis
Muzny, Christina A.; Taylor, Christopher M.; Swords, W. Edward; Tamhane, Ashutosh; Chattopadhyay, Debasish; Cerca, Nuno; Schwebke, Jane R.Bacterial vaginosis (BV) is the most common cause of vaginal discharge. It is associated with an increased risk of preterm delivery, pelvic inflammatory disease, and an increased risk of acquisition of sexually transmitted infections including human immunodeficiency virus (HIV). The epidemiology of BV supports sexual transmission. However, its etiology remains unknown. At the center of the debate is whether BV ...