8 documents found, page 1 of 1
Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in t...
Lamy, Elsa; Rubio, Camila; Carreira, Laura; Capela e Silva, Fernando; Martinez-Subiela, Silvia; Tecles, Fernando; Lopez-Jornet, Pia; Céron, JoseThe present study aims to assess the effects of thermal and chemical inactivating procedures, that can be used for SARS-CoV-2 inactivation, on different salivary analytes. SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) protein profile and a panel of 25 specific biomarkers of oxidative status, stress, metabolism and tissue damage were evaluated in samples subjected to different treatments: thermal (65 °C or 9...
Changes in saliva analytes in dairy cows during peripartum: A pilot study
Contreras-Aguilar, Maria Dolores; Vallejo-Mateo, P.J.; Lamy, Elsa; Escribano, Damian; Ceron, Jose Joaquim; Tecles, Fernando; Rubio, CamilaThis pilot study aimed to study the possible changes in a profile of 26 salivary analytes in thirteen healthy dairy cows along their peripartum period. Analytes associated with the stress (salivary cortisol, salivary alpha-amylase, butyrylcholinesterase, and total esterase), inflammation (adenosine deaminase), oxidative status (total antioxidant capacity and the advanced oxidation protein products), and general...
Analytical validation of an automated assay for the measurement of adenosine de...
Franco-Martinéz, Lorena; Tecles, Fernando; Torres-Cantero, A.; Bernal, E.; San-Lázaro, I.; Alcaraz, M.J.; Vicente-Romero, M.R.; Lamy, ElsaObjectives The aim of the present study was to validate a commercially available automated assay for the measurement of total adenosine deaminase (tADA) and its isoenzymes (ADA1 and ADA2) in saliva in a fast and accurate way, and evaluate the possible changes of these analytes in individuals with SARS-CoV-2 infection. Methods The validation, in addition to the evaluation of precision and accuracy, included the ...
Changes in saliva analytes in dairy cows during peripartum: A pilot study
Contreras-Aguilar, María D.; Vallejo-Mateo, Pedro J.; Lamy, Elsa; Escribano, Damián; Cerón, Jose J.; Tecles, Fernando; Rubio, Camila P. [UNESP]Made available in DSpace on 2021-06-25T10:54:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-01; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Universidade Estadual Paulista; Fundação para a Ciência e a Tecnologia; Universidad de Murcia; This pilot study aimed to study the possible changes in a profile of 26 salivary analytes in thirteen healthy dairy cows along their peripar...
Changes in saliva analytes in equine acute abdominal disease: a sialochemistry ...
Contreras-Aguilar, Maria Dolores; Escribano, Damian; Martínez-Subiela, Silvia; Martín-Cuervo, María; Lamy, Elsa; Tecles, Fernando; Ceron, JoseBackground: The biochemical components of saliva can change in certain pathologies in horses, for example in acute abdominal disease. The aim of this study was (1) to evaluate if a panel of biochemical analytes usually used in serum can be measured in saliva of horses and (2) to study the possible changes of these biochemical analytes in saliva of horses affected by acute abdominal disease. A panel of 23 analyt...
Changes of salivary biomarkers under different storage conditions: effects of t...
Barranco, Tomás; Rubio, Camila P; Tvarijonaviciute, Asta; Rubio, Mónica; Damia, Elena; Lamy, Elsa; Cugat, Ramón; Cerón, José J; Tecles, FernandoIntroduction In this report, we aimed to examine the stability of various analytes in saliva under different storage conditions. Materials and methods Alpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric red...
Development and validation of an assay for measurement of leptin in pig saliva
Schmidt, Elizabeth M.S. [UNESP]; Escribano, Damián; Martinez-Subiela, Silvia; Martinez-Miró, Silvia; Hernández, Fuensanta; Tvarijonaviciute, AstaMade available in DSpace on 2018-12-11T17:07:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-10-28; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Background: Leptin has been measured in human in saliva samples. However, the low leptin concentration found in this biological fluid makes necessary the use of high sensitive methods. To the authors' knowledge, leptin has not been me...
Changes in biochemical analytes in calves infected by nematode parasites in fie...
de Cezaro, Marcela C. [UNESP]; Tvarijonaviciute, Asta; Tecles, Fernando; Céron, José J.; Eckersall, David P.; Ferreira, João C.P. [UNESP]Made available in DSpace on 2018-12-11T17:01:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-03-30; Parasitic infections caused by nematodes are a major problem in bovines that resulting in losses in animal health and production. Thus, the aim of this study was to evaluate alterations in selected serum biochemical analytes in calves naturally infected with gastrointestinal (GI) and pulmonary nematodes...