14 documents found, page 1 of 2
Guidance on minimum information requirements (MIR) from designing to reporting ...
Jeddi, Maryam Zare; Galea, Karen S.; Ashley-Martin, Jillian; Nassif, Julianne; Pollock, Tyler; Poddalgoda, Devika; Kasiotis, Konstantinos M.Human biomonitoring (HBM) provides an integrated chemical exposures assessment considering all routes and sources of exposure. The accurate interpretation and comparability of biomarkers of exposure and effect depend on harmonized, quality-assured sampling, processing, and analysis. Currently, the lack of broadly accepted guidance on minimum information required for collecting and reporting HBM data, hinders co...
Measuring DNA modifications with the comet assay: a compendium of protocols
Collins, Andrew; Moller, Peter; Gajski, Goran; Vodenkova, Sona; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, StefanoThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there ...
From inequitable to sustainable e-waste processing for reduction of impact on h...
Ádám, Balázs; Göen, Thomas; Scheepers, Paul T.J.; Adliene, Diana; Batinic, Bojan; Budnik, Lygia T.; Duca, Radu-Corneliu; Ghosh, ManosijRecycling of electric and electronic waste products (e-waste) which amounted to more than 50 million metric tonnes per year worldwide is a massive and global operation. Unfortunately, an estimated 70-80% of this waste has not been properly managed because the waste went from developed to low-income countries to be dumped into landfills or informally recycled. Such recycling has been carried out either directly ...
Prenatal exposure to tobacco smoke: H2AX phosphorylation levels in umbilical co...
Duarte, Xavier; Silva, Ana Inês; Reis, Ana Teresa; Teixeira, Joao Paulo; Madureira, Joana; Costa, CarlaThe curent study.carried out within the frame of the NeoGene project, examined the effects of prenatal tobacco smoke onphosphorylation levels of the histone variant H2AX, a reliable biomarker of the formation of DNA double strand breaks inumbilical cord blood.
Assessment of genotoxic effects of titanium dioxide nanoparticles on different ...
Fernández-Bertólez, Natalia; Brandao, Fátima; Rosário, Fernanda; Bessa, Maria João; Fraga, Sónia; Pásaro, Eduardo; Teixeira, Joao Paulo; Costa, CarlaThe main objective of the present work was to assess the cellular uptake and potential genotoxicity (micronuclei induction) of TiO2 NPs on four diverse human cell lines.
Association of inflammatory mediators with frailty status in older adults: resu...
Marcos-Pérez, Diego; Sánchez-Flores, María; Proietti, Stefania; Bonassi, Stefano; Costa, Solange; Teixeira, Joao Paulo; Fernández-Tajes, JuanFrailty is a geriatric syndrome defined as a status of extreme vulnerability to stressors, leading to a higher risk of negative health-related outcomes. "Inflammaging", an age-related state of low-grade chronic inflammation, is characterized by an increased concentration of pro-inflammatory cytokines and acute phase proteins. Inflammaging has been postulated as an underlying mechanism of frailty, and several st...
Genotoxicity study of human astrocytes exposed to oleic acid-coated iron oxide ...
Valdiglesias, Vanessa; Fernández-Bertólez, Natalia; Costa, Carla; Brandão, Fátima; Teixeira, Joao Paulo; Pásaro, Eduardo; Laffon, BlancaIron oxide nanoparticles (ION) have attracted much attention in last decade for their utility in several biomedical applications such as magnetic resonance imaging, hyperthermia-based cancer therapy, and targeted drug/gene delivery. Particularly, previous works showed that ION can cross the blood-brain barrier, making them especially useful as diagnostic and therapeutic tools, specifically in central nervous sy...
Low Vitamin D Levels and Frailty Status in Older Adults: A Systematic Review an...
Marcos-Pérez, Diego; Sánchez-Flores, María; Proietti, Stefania; Bonassi, Stefano; Costa, Solange; Teixeira, Joao Paulo; Fernández-Tajes, JuanSerum vitamin D deficiency is widespread among older adults and is a potential modifiable risk factor for frailty. Moreover, frailty has been suggested as an intermediate step in the association between low levels of vitamin D and mortality. Hence, we conducted a systematic review of the literature and meta-analysis to test the possible association of low concentrations of serum 25-hydroxyvitamin D (25(OH)D), a...
Evaluation of cytotoxicity and genotoxicity induced by oleic acid-coated iron o...
Fernández-Bertólez, Natalia; Costa, Carla; Brandão, Fátima; Duarte, José Alberto; Teixeira, Joao Paulo; Pásaro, Eduardo; Valdiglesias, VanessaIron oxide nanoparticles (ION) are gaining importance as diagnostic and therapeutic tool of central nervous system diseases. Although oleic acid-coated ION (O-ION) have been described as stable and biocompatible, their potential neurotoxicity was scarcely evaluated in human nervous cells so far. The primary aim of this work was to assess the molecular and cellular effects of O-ION on human astrocytes (A172 cell...
Toxicological assessment of silica-coated iron oxide nanoparticles in human ast...
Fernández-Bertólez, Natalia; Costa, Carla; Brandão, Fátima; Kiliç, Gözde; Duarte, José Alberto; Teixeira, Joao Paulo; Pásaro, EduardoIron oxide nanoparticles (ION) have great potential for an increasing number of medical and biological applications, particularly those focused on nervous system. Although ION seem to be biocompatible and present low toxicity, it is imperative to unveil the potential risk for the nervous system associated to their exposure, especially because current data on ION effects on human nervous cells are scarce. Thus, ...