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Time-dependent accumulation of biogenic amines and microbial succession during ...

Ribeiro, Ana J.; Milheiro, Juliana; Nunes, Fernando M.; Carvalho, Teresa B. de; Barbosa, Joana B.; Silva, Filipe; Teixeira, Paula; Saraiva, Cristina M.

Dry-aging of beef under controlled temperature, humidity and airflow reshapes the surface microbiota and may influence biogenic amine (BA) accumulation. In this study, culture-based enumeration, 16 S rRNA gene profiling of combined crust + inner meat, and validated HPLC quantification of BAs were integrated to track safety- and quality-relevant changes over 60 days. Sequencing showed Pseudomonadota and Bacillot...


Nitrite reduction in cooked ham: an organoleptic and food safety concern?

Nunes, Maria J. M.; Pereira, Rui C.; Noronha, Lúcia; Cruz, Inês; Komora, Norton; Barbosa, Joana Bastos; Monteiro, Maria João P.; Ribas, Tânia C. F.

Cooked meat products, particularly ham, are widely consumed, and reducing nitrite levels has become a priority due to health concerns and regulatory pressure. This study evaluated the microbiological safety, technological performance, physicochemical properties, and sensory attributes of whole cooked ham formulated with reduced nitrite (from 150 to 80 ppm) during shelf life. Microbiological analyses were conduc...


Extending the shelf-life of live clams, Venerupis corrugata - important aspects...

Goes, Cintia Borghetti; Teixeira, Susana; Mena, Cristina; Silva, Fátima; Cruz, Andreia; Basílio, Inês; Hogg, Maria Conceição; Sivertsvik, Morten

Venerupis corrugata (pullet carpet shell) is a premium native clam species in Portugal. This species is highly perishable, typically sold live within 3 or 4 days, posing a significant risk of loss. Therefore, efforts to extend its shelf-life are relevant. The impact of the storage temperature (3, 5, 8 and 12 °C) on clams in plastic net bags and the effect of modified atmosphere packaging (MAP) were investigated...


O inimigo invisível resistente que desafia a indústria alimentar

Almeida, Gonçalo; Magalhães, Rui; Sousa, Pedro; Noronha, Lúcia; Sousa, Ana; Teixeira, Paula


Impact of polysorbate 80 on the antimicrobial activity of oregano and thyme

Carvalho, Marta; Barbosa, Joana; Silva, Marcelo Belchior Rosendo da; Albano, Helena; Teixeira, Paula

Plant-derived essential oils (EOs) possess significant antimicrobial potential against spoilage and pathogenic microorganisms. However, their efficacy can vary depending on the test method, making it difficult to standardise results. This study aimed to investigate the effect of polysorbate 80, a common surfactant used to emulsify EOs, on antimicrobial activity and minimum inhibitory concentration (MIC) determi...


Impact of red wine vinegar-based solution on bacterial communities of squid and...

Rezende, Lourenço Pinto de; Barbosa, Joana Bastos; Carvalho, Teresa Bento de; Vaz-Moreira, Ivone; Monteiro, Maria João P.; Teixeira, Paula

Seafood is an essential component of a balanced and healthy diet, which increases its demand. However, its biological composition and high moisture content make these products extremely perishable. To prevent spoilage, and the consequent food waste and fnancial expenses throughout the seafood supply chain, new technologies have been successfully developed to inhibit bacterial growth, the main cause of seafood s...


Natural preservation strategies for fruit: based bakery fillings

Teixeira, Paula; Tomé, Elisabetta; Carvalho, Maria Teresa Bento de; Sousa, Pedro; Oliveira, Isabel; Azevedo, Miguel

The storage stability of an industrial banana-based filling commonly used in bakery applications was evaluated over 70 days at 22 °C and 62% relative humidity. Potassium sorbate (0.095%) was replaced with a commercial red fruit extract (0.5%) as a natural preservative. Microbiological characteristics, including total viable counts, molds and yeasts and spore-forming bacilli, were assessed throughout storage. Ad...


Impact of fermentation on the antioxidant activity and color of haematococcus p...

Bassani, Joseane C.; Martins, Valter; Barbosa, Joana; Steffens, Juliana; Backes, Geciane T.; Pereira, Hugo; Pintado, Manuela E.; Morais, Alcina M. M. B.

Microalgae are emerging as a promising ingredient for the food industry due to their sustainable cultivation and production of a variety of bioactive compounds, including proteins, lipids, carbohydrates, antioxidant compounds, and pigments [1]. Among the many cataloged species of microalgae, two stand out in research due to their powerful applications in the pharmaceutical, nutraceutical, cosmetic, and food and...


Lemon dietary fibre-based powder as a promising ingredient for the food industr...

Magalhães, Daniela; Rodrigues, Cristina V.; Botella-Martinez, Carmen; Muñoz-Tebar, Nuria; Pérez-Álvarez, José Angel; Viuda-Martos, Manuel

Lemon co-products are valuable due to their high dietary fibre, making them significant for valorisation. This research aimed to characterise an innovative lemon dietary fibre (LDF) obtained through integrated extraction (of essential oil, phenolic compounds (PCs), and pectin) by evaluating its chemical, physicochemical, structural, techno-functional, total phenolic content, and antioxidant and antibacterial pr...


Evaluating biosolutions for sustainable food systems: a review of safety, quali...

Thomsen, Marianne; Leroi, Françoise; Passerini, Delphine; Siemiatkowska, Milena; Sopacua, Tirzania; Andersson, Kristina; Teixeira, Paula; Poças, Fatima

Biosolutions, such as food cultures and fermentates, are emerging alternatives to classical food preservation methods, offering multiple benefits, including food quality and safety enhancement, and waste reduction, with limited sensory and nutritional modification of the product and higher consumer acceptance. This study explores the potential of biosolutions to address critical challenges in sustainable food s...


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