5 documents found, page 1 of 1
Cryogenic, but not hypothermic, preservation disrupts the extracellular matrix ...
Ribeiro, Sara Cristina Freitas; Carvalho, A. F.; Rodrigues, Daniel Barreira; Martins, L.; Pires, R. A.; Mendes, V. M.; Manadas, B.; Jarnalo, M.Cell sheet (CS)-based approaches hold significant potential for tissue regeneration, relying on the extracellular matrix (ECM) for success. Like in native tissues, the ECM provides structural and biochemical support for cellular homeostasis and function. Effective preservation strategies that maintain ECM integrity are critical to enhance the therapeutic potential of CS-based approaches. While cryogenic and hyp...
Proteomics of sea bass skin-scales exposed to the emerging pollutant fluoxetine...
Anjos, L.; Estêvão, Dulce; Santos, S.; Santa, C.; Manadas, B.; Monsinjon, T.; Canario, Adelino; Power, Deborah; Pinto, Patricia ISTeleost fish skin-scales are essential for protection and homeostasis and the largest tissue in direct contact with the environment, but their potential as early indicators of pollutant exposure are hampered by limited knowledge about this model. This study evaluated multi-level impacts of in vivo exposure of European sea bass to fluoxetine (FLX, a selective serotonin-reuptake inhibitor and an emerging pollutan...
Effects of proteolytic digestion on the cyanotoxins microcystin-LR and cylindro...
Freitas, Marisa; Azevedo, J.; Carvalho, A.P.; Mendes, V.M.; Manadas, B.; Campos, A.; Vasconcelos, V.The occurrence and proliferation of toxic cyanobacterial blooms are an emergent environmental concern worldwide. Microcystin-LR (MC-LR), a potent hepatotoxin, is the most documented and studied cyanotoxin. The cytotoxin cylindrospermopsin (CYN) has been recognized of increased concern due to the invasive nature of its main producer, Cylindrospermopsis raciborskii. Previous studies have shown that edible aquatic...
Unveiling the effects of the secretome of mesenchymal progenitors from the umbi...
Fraga, J. S.; Silva, N. A.; Lourenço, A. S.; Gonçalves, V.; Neves, N. M.; Reis, R. L.; Rodrigues, A. J.; Manadas, B.; Sousa, N.; Salgado, A. J.It has been previously shown that the secretome of Human Umbilical Cord Perivascular Cells (HUCPVCs), known for their mesenchymal like stem cell character, is able to increase the metabolic viability and hippocampal neuronal cell densities. However, due to the different micro-environments of the distinct brain regions it is important to study if neurons isolated from different areas have similar, or opposite, r...
Unveiling the effects of the secretome of mesenchymal progenitors from the umbi...
Fraga, J. S.; Silva, N. A.; Lourenço, A. S.; Gonçalves, V.; Neves, N. M.; Reis, R. L.; Rodrigues, A. J.; Manadas, B.; Sousa, N.; Salgado, A. J.It has been previously shown that the secretome of Human Umbilical Cord Perivascular Cells (HUCPVCs), known for their mesenchymal like stem cell character, is able to increase the metabolic viability and hippocampal neuronal cell densities. However, due to the different micro-environments of the distinct brain regions it is important to study if neurons isolated from different areas have similar, or opposite, r...