10 documents found, page 1 of 1
Neurodifferentiation and neuroprotection potential of mesenchymal stromal cell-...
Marques, Cláudia Raquel; Fuzeta, Miguel de Almeida; Cunha, Raquel Medina dos Santos; Pereira-Sousa, Joana; Silva, Deolinda; Campos, Jonas OliveiraParkinson’s disease (PD) is the second most common neurodegenerative disorder and is characterized by the degeneration of the dopamine (DA) neurons in the substantia nigra pars compacta, leading to a loss of DA in the basal ganglia. The presence of aggregates of alpha-synuclein (α-synuclein) is seen as the main contributor to the pathogenesis and progression of PD. Evidence suggests that the secretome of mesenc...
Microglial depletion has no impact on disease progression in a mouse model of m...
Campos, Ana Bela; Silva, Sara Carina Duarte; Fernandes, Bruno; Coimbra, Bárbara; Campos, Jonas; Monteiro-Fernandes, Daniela; Teixeira-Castro, AndreiaMachado–Joseph disease (MJD), also known as spinocerebellar ataxia type 3 (SCA3), is an autosomal dominant neurodegenerative disorder (ND). While most research in NDs has been following a neuron-centric point of view, microglia are now recognized as crucial in the brain. Previous work revealed alterations that point to an increased activation state of microglia in the brain of CMVMJD135 mice, a MJD mouse model ...
Data on the effects of Hyptis spp. and Lycium spp. plant extracts in C. elegans...
Vilasboas-Campos, Daniela; Costa, Marta Daniela; Teixeira-Castro, Andreia; Rios, Rejaine; Silva, Fabiano Guimarães; Aierken, Aili; Zhang, XiaoyingHere, we present the data on the biological effects of Hyptis spp. and Lycium spp. plant extracts in Caenorhabditis elegans (C. elegans) models of neurodegenerative diseases, which is related to the work presented in the article "Neurotherapeutic effect of Hyptis spp. leaf extracts in Caenorhabditis elegans models of tauopathy and polyglutamine disease: role of the glutathione redox cycle" [1]. This dataset was...
Phospholipase D functional ablation has a protective effect in an Alzheimer's d...
Bravo, Francisca Vaz; Da Silva, Jorge; Chan, Robin Barry; Di Paolo, Gilbert; Teixeira-Castro, Andreia; Oliveira, Tiago GilPhospholipase D (PLD) is a key player in the modulation of multiple aspects of cell physiology and has been proposed as a therapeutic target for Alzheimer's disease (AD). Here, we characterize a PLD mutant, pld-1, using the Caenorhabditis elegans animal model. We show that pld-1 animals present decreased phosphatidic acid levels, that PLD is the only source of total PLD activity and that pld-1 animals are more ...
Limited Effect of Chronic Valproic Acid Treatment in a Mouse Model of Machado-J...
Esteves, Sofia; Duarte-Silva, Sara; Naia, Luana; Neves-Carvalho, Andreia; Teixeira-Castro, Andreia; Rego, Ana Cristina; Silva-Fernandes, AnabelaMachado-Joseph disease (MJD) is an inherited neurodegenerative disease, caused by a CAG repeat expansion within the coding region of ATXN3 gene, and which currently lacks effective treatment. In this work we tested the therapeutic efficacy of chronic treatment with valproic acid (VPA) (200mg/kg), a compound with known neuroprotection activity, and previously shown to be effective in cell, fly and nematode model...
An image processing application for quantification of protein aggregates in Cae...
Teixeira-Castro, Andreia; Dias, Nuno; Rodrigues, Pedro; Oliveira, João Filipe; F. Rodrigues, Nuno; Maciel, Patrícia; Vilaça, João L.Protein aggregation became a widely accepted marker of many polyQ disorders, including Machado-Joseph disease (MJD), and is often used as readout for disease progression and development of therapeutic strategies. The lack of good platforms to rapidly quantify protein aggregates in a wide range of disease animal models prompted us to generate a novel image processing application that automatically identifies and...
Automatic segmentation and 3D feature extraction of protein aggregates in Caeno...
Rodrigues, Pedro L.; Moreira, António H. J.; Teixeira-Castro, Andreia; Oliveira, João; Dias, Nuno; F. Rodrigues, Nuno; Vilaça, João L.In the last years, it has become increasingly clear that neurodegenerative diseases involve protein aggregation, a process often used as disease progression readout and to develop therapeutic strategies. This work presents an image processing tool to automatic segment, classify and quantify these aggregates and the whole 3D body of the nematode Caenorhabditis Elegans. A total of 150 data set images, containing ...
Development and characterization of PHB-HV based 3D scaffolds for a tissue engi...
Samy, S. M.; Silva, Nuno A.; Correlo, Vitor M.; Fraga, Joana S.; Pinto, Luísa; Teixeira-Castro, Andreia; Leite-Almeida, Hugo; Almeida, ArmandoSpinal cord injury (SCI) leads to devastating neurological deficits. Several tissue engineering (TE)- based approaches have been investigated for repairing this condition. Poly (3-hydroxybutyrateco- 3-hydroxyvalerate) (PHB-HV) is found to be particularly attractive for TE applications due to its properties, such as biodegradability, biocompatibility, thermoplasticity and piezoelectricity. Hence, this report add...
NEDD8: A new ataxin-3 interactor
Ferro, Anabela; Carvalho, Ana Luísa; Teixeira-Castro, Andreia; Almeida, Carla; Tomé, Ricardo J.; Cortes, Luísa; Rodrigues, Ana-João; Logarinho, ElsaMachado-Joseph disease (MJD/SCA3) is an autosomal dominant neurodegenerative disease caused by the expansion of a CAG tract in the coding portion of the ATXN3 gene. The presence of ubiquitin-positive aggregates of the defective protein in affected neurons is characteristic of this and most of the polyglutamine disorders. Recently, the accumulation of the neural precursor cell expressed developmentally downregul...
Exclusion of mutations in the PRNP, JPH3, TBP, ATN1, CREBBP, POU3F2 and FTL gen...
Costa, Maria do Carmo; Teixeira-Castro, Andreia; Constante, Marco; Magalhães, Marina; Magalhães, Paula; Cerqueira, Joana; Vale, José; Passão, VitorinaHuntington disease (HD) is an autosomal dominant neurodegenerative disorder characterised by chorea, cognitive impairment, dementia and personality changes, caused by the expansion of a CAG repeat in the HD gene. Often, patients with a similar clinical presentation do not carry expansions of the CAG repeat in this gene [Huntington disease-like (HDL) patients]. We report the genetic analysis of 107 Portuguese pa...