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Effects of nanostructure antifouling biocides towards a coral species in the co...

Ferreira, Violeta; Pavlaki, Maria D.; Martins, Roberto; Monteiro, Marta S.; Maia, Frederico; Tedim, João; Soares, Amadeu M. V. M.; Calado, Ricardo

Biofouling prevention is one of the biggest challenges faced by the maritime industry, but antifouling agents commonly impact marine ecosystems. Advances in antifouling technology include the use of nanomaterials. Herein we test an antifouling nano-additive based on the encapsulation of the biocide 4,5-dichloro-2-octyl-4-isothiazolin-3-one (DCOIT) in engineered silica nanocontainers (SiNC). The work aims to ass...


Effects of ultraviolet radiation to Solea senegalensis during early development

Araújo, Mário J.; Quintaneiro, Carla; Soares, Amadeu M. V. M.; Monteiro, Marta S.

Ultraviolet radiation (UVR) reaching the Earth surface is increasing and scarce information is available regarding effects of this stressor to early life stages of marine vertebrates. Therefore, this work aims to study the effects of UVR exposure during early development stages of the flatfish Solea senegalensis. Firstly, fish were exposed to UVR (six daily doses between 3.4 ± 0.08 and 8.6 ± 0.14 kJ m-2) at the...


Impact of wastewater-borne nanoparticles of silver and titanium dioxide on the ...

Galhano, Victor; Hartmann, Sarah; Monteiro, Marta S.; Zeumer, Richard; Mozhayeva, Darya; Steinhoff, Benedikt; Müller, Katharina; Prenzel, Kirsten

Due to their widespread use, silver (Ag) and titanium dioxide (TiO2) nanoparticles (NPs) are commonly discharged into aquatic environments via wastewater treatment plants. The study was aimed to assess the effects of wastewater-borne AgNPs (NM-300 K; 15.5 ± 2.4 nm; 25-125 μg L-1) and TiO2NPs (NM-105; 23.1 ± 6.2 nm; 12.5-100 μg L-1), from a laboratory-scale wastewater treatment plant, on Daphnia magna, at indivi...


The use of comet assay to assess global DNA methylation in human biomonitoring ...

Costa, Carla; Alves, Ana Catarina; Costa, Solange; Soares, Amadeu M.V.M.; Monteiro, Marta S.; Loureiro, Susana; Teixeira, João Paulo

The Comet assay is a valuable tool for the detection of DNA damage in genotoxicity and human biomonitoring studies. Throughout the years, this biomarker has undergone several adaptations in their protocol in order to increase its sensitivity and the possible outcomes. By including an additional step of DNA digestion with lesion-specific endonucleases, the comet assay can provide information regarding the type o...


Biomarkers of endocrine disruption in juveniles and females of the estuarine fi...

Dias, Lídia C. A.; Soares, Amadeu M. V. M.; Ferreira, Abel L. G.; Santos, Cátia S.; Monteiro, Marta S.

The presence of endocrine disruptor chemicals (EDCs) in aquatic compartments, including estuaries, has been object of major concern. However, the effects of EDCs on autochthonous estuarine fish species are far less known than in freshwater fish. Therefore, the response of biomarkers in the estuarine fish Pomatoschistus microps was evaluated after 21-days of exposure to different EDCs: 17β-estradiol (E2); PCB-77...


Characterization of cholinesterases in Chironomus riparius and the effects of t...

Pérez, Joanne; Monteiro, Marta S.; Quintaneiro, Carla; Soares, Amadeu M. V. M.; Loureiro, Susana

In this study, the toxicities of four pesticides (the herbicides atrazine, terbuthylazine, metolachlor and the insecticide chlorpyrifos) previously detected in the Alqueva reservoir/dam (south of Portugal) were evaluated individually and in binary combinations of the herbicides and the insecticide using fourth-instar larvae of the aquatic midge Chironomus riparius.Chlorpyrifos induced toxicity to midges in all ...


Characterization of cholinesterases in plasma of three Portuguese native bird s...

Santos, Cátia S. A.; Monteiro, Marta S.; Soares, Amadeu M. V. M.; Loureiro, Susana

Over the last decades the inhibition of plasma cholinesterase (ChE) activity has been widely used as a biomarker to diagnose organophosphate and carbamate exposure. Plasma ChE activity is a useful and non-invasive method to monitor bird exposure to anticholinesterase compounds; nonetheless several studies had shown that the ChE form(s) present in avian plasma may vary greatly among species. In order to support ...


The influence of metal speciation on the bioavailability and sub-cellular distr...

Calhoa, Filipa Calhôa; Monteiro, Marta S.; Soares, Amadeu M. V. M.; Mann, Reinier M.

Cadmium is a non-essential toxic metal that is able to bioaccumulate in both flora fauna and has the potential to biomagnify in some food chains. However, the form in which cadmium is presented to consumers can alter the bioavailability and possibly the internal distribution of assimilated Cd. Previous studies in our laboratory highlighted differences in Cd assimilation among isopods when they were provided wit...


Does subcellular distribution in plants dictate the trophic bioavailability of ...

Monteiro, Marta S.; Santos, Conceição; Soares, Amadeu M. V. M.; Mann, Reinier M.

The present study examined how subcellular partitioning of Cd in plants with different strategies to store and detoxify Cd may affect trophic transfer of Cd to the isopod Porcellio dilatatus. The plant species used were Lactuca sativa, a horticultural metal accumulator species; Thlaspi caerulescens, a herbaceous hyperaccumulator species; and the nonaccumulator, T. arvense. Taking into account that differences i...


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