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Bacterial contamination of gutta-percha points from different brands and the ef...

Bracciale, Francesca; Marino, Nicole; Noronha, Anariely; Manso, M. Conceição; Gavinha, Sandra; Cardoso, Inês Lopes

Objective: To evaluate the bacterial contamination of different brands of Gutta-Percha (GP) points routinely used in clinical practice and the efficacy of a chairside disinfection protocol with sodium hypochlorite. Methods: GP points (n=240), in sizes A, B, C, D, K15, K20, K25, K30, K35, K40, F1, F2, F3 (Dentsply®, Proclinic®, ProTaper® and R&S®), were randomly sampled from commercial packages already in use. T...


Oral microbiome associated with peri-implant infections – a review

Molina, Flávio; Cardoso, Inês Lopes; Teles, Ana Moura; Pina, Cristina Maria San Román Gomes de

Dental treatments using dental implants have been well documented over the past 40 years and with great success. The dental implant installed in the place of missing teeth should always involve proper forecasting by the dentist. Namely, it is important to know the microbiome surrounding the implant, from its planning till final rehabilitation. The exact time of microbiome formation, as well as microorganisms in...


Apical periodontitis and related risk factors: cross-sectional study

Correia-Sousa, Joana; Madureira, Ana Raquel; Carvalho, Manuel Fontes; Teles, Ana Moura; Pina-Vaz, Irene

Objectives: The aim of this cross-sectional study was to investigate an association between the prevalence of root-filled teeth (RFT) or apical periodontitis (AP) and some systemic conditions or smoking habits in an adult Portuguese population. Methods: Medical histories, including age, gender, presence of cardiovascular disease (CVD), diabetes mellitus (DM), allergies, smoking status, and endodontic treatment ...


In vivo evaluation of microbial reduction after chemo-mechanical preparation of...

Teles, Ana Moura; Manso, M. Conceição; Pina, Cristina Maria San Román Gomes de; Cabeda, José Manuel

Objectives: Assessment of bacterial reduction after chemo-mechanical preparation (using 3% sodium hypochlorite) with or without intracanal dressing (calcium hydroxide paste (Ca(OH)2) or 2% chlorhexidine digluconate gel (CHX)) in necrotic pulps associated or not with apical lesion. Methods: Prospective clinical trial, in 69 adult patient’s teeth with pulpal necrosis associated or not with apical periodontitis. M...


Identification of yeast and non-pigmented cultivable endodontic bacteria in adu...

Teles, Ana Moura; Manso, M. Conceição; Loureiro, Sara Manuela Marinho; Silva, Ricardo; Madeira, Inês; Pina, Cristina Maria San Román Gomes de

Introduction This study has focused on the identification of the yeasts and non-pigmented bacteria present on adult patients with necrosis or apical periodontitis and the ones who resisted chemomechanical preparation and intracanal dressing with calcium hydroxide paste (Ca(OH)2) or 2% chlorohexidine digluconate gel (CHX). Methods 69 single-rooted teeth of adult patients with necrosis associated or not with apic...


Microorganisms: the reason to perform endodontics

Teles, Ana Moura; Manso, M. Conceição; Loureiro, Sara; Pina, Cristina Maria San Román Gomes de; Cabeda, José Manuel

That we perform Endodontics because there are microorganisms is now beyond doubt. Nevertheless, not only the microorganisms, but also the host response have a profound effect on the progression of the disease. Many papers confirmed the polymicrobial nature of pulpal and periapical diseases of endodontic origin and the efficiency of the chemo-mechanical procedures based on physical and chemical elimination of th...


Effectiveness of two intracanal dressings in adult portuguese patients: a qPCR ...

Teles, Ana Moura; Manso, M. Conceição; Loureiro, S.; Silva, R.; Madeira, I. G. C.; Pina, C.; Cabeda, José Manuel

Aim: To quantify bacterial equivalents before and after chemomechanical preparation using 3% sodium hypochlorite (NaOCl) and intracanal dressing with calcium hydroxide paste (Ca(OH)2 ) or 2% Chlorhexidine digluconate gel (CHX) in necrotic pulps associated or not with apical periodontitis and to further compare this quantification with counts of anaerobic microorganisms. Methodology: Prospective clinical trial i...


A review of microbiologic root canal sampling: updating an emerging picture

Teles, Ana Moura; Manso, M. Conceição; Pina, Cristina Maria San Román Gomes de; Cabeda, José Manuel

Objectives: The significance of microorganisms in root canals with regard to the aetiology of periapical infection and the need for crucial bacteria control during treatment are undeniable. In this study, we report and discuss a review of the literature on Microbiological Root Canal Sampling (MRS). The procedure is analyzed in detail, discussing its powers, limitations and the influence of sample collection pro...


Estudo comparativo da capacidade de selamento de três técnicas de obturação de ...

Teles, Ana Moura; Paulo, Manuel Fonseca; Capelas, José António; Melo, Paulo; Cunha, Luís Miguel

O objectivo deste estudo in vitro foi avaliar, subjacente à observação da infiltração apical, a capacidade de selamento de três técnicas de obturação: “condensação lateral”, “thermafil” e “soft-core” e, em função dos resultados, poder concluir qual proporciona melhores garantias de isolamento hermético do sistema de canais radiculares. Nesse sentido, foram preparados 49 dentes monorradiculares, dos quais, 45 fo...


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