2 documents found, page 1 of 1
Targeted massively parallel sequencing for congenital generalized lipodystrophy
Costa-Riquetto,Aline D.; Santana,Lucas S.; Caetano,Lílian A.; Lerário,Antônio M.; Correia-Deur,Joya E. M.; Bertola,Débora R.; Kim,Chong A.; Nery,MárciaABSTRACT Objective: Our aim is to establish genetic diagnosis of congenital generalized lipodystrophy (CGL) using targeted massively parallel sequencing (MPS), also known as next-generation sequencing (NGS). Subjects and methods: Nine unrelated individuals with a clinical diagnosis of CGL were recruited. We used a customized panel to capture genes related to genetic lipodystrophies. DNA libraries were generated...
Cyclosporin safety in a simplified rat brain tumor implantation model
Felix,Francisco H. C.; Fontenele,Juvenia B.; Teles,Milena G.; Bezerra Neto,João E.; Santiago,Márcia H. A. M.; Picanço Filho,Roberto L.Brain cancer is the second neurological cause of death. A simplified animal brain tumor model using W256 (carcinoma 256, Walker) cell line was developed to permit the testing of novel treatment modalities. Wistar rats had a cell tumor solution inoculated stereotactically in the basal ganglia (right subfrontal caudate). This model yielded tumor growth in 95% of the animals, and showed absence of extracranial met...