Efficiently valorizing olive tree prunings (OTP) for microbial lipids represents an excellent strategy to achieve resource circularity, accompanied by economic and environmental benefits. Since the production of lipids from OTP hydrolysate (OTPH) by yeasts remains underexplored, this study investigated the potential of Y. lipolytica to produce lipid-rich biomass in bioreactor batch cultures. OTP was fractionate...
Seaweed farming and production have grown significantly, driven by their use for human consumption and the extraction of bioactive compounds with application in the feed, pharmaceutical, cosmetic, and textile industries. Palmaria palmata, a seaweed native to the Atlantic coast of Europe, is widely explored for the extraction of proteins and polyphenols. The residual seaweed biomass obtained after compounds extr...
Atualmente, a procura por novas fontes alimentares está a crescer de forma significativa, com o objetivo de responder à expectável e crescente necessidade de alimentos nos próximos anos. Nesse sentido, a FAO (Organização das Nações Unidas para Alimentação e Agricultura) e a União Europeia têm promovido a incorporação de insetos como uma alternativa de proteína sustentável e altamente nutritiva para o consumo hu...
Soybean molasses (SM), a sugar-rich by-product obtained from manufacturing soy protein concentrate, is an interesting source of fermentable sugars for biotechnological processes. For the first time, SM was used as a new and low-cost substrate in Aspergillus niger cultures to produce gluconic acid (GA), a carboxylic acid obtained by the oxidation of glucose and with a wide range of applications. The effect of SM...
Aureobasidium pullulans NCYC 4012 was used to produce gluconic acid (GA) and fructo-oligosaccharides (FOS) from sugarcane molasses (ScM). For the first time, three types of bioreactors operating at different conditions of oxygenationa stirred tank reactor (STR) operating under atmospheric pressure varying stirring rate, a pressurized STR under increased air pressure up to 4 bar of air, and an airlift reactor un...
Eucalyptus bark hydrolysate (EBH) is an abundant and renewable source of fermentable sugars for microbial lipids production. However, microorganisms need to be tolerant to antimicrobial compounds present in these type-hydrolysates and formed during lignocellulosic biomass processing. The ability of Y. lipolytica W29 to grow and produce lipids in undetoxified and undiluted EBH was evaluated in stirred tank biore...
The bioconversion of glucose to gluconic acid (GA) by Aureobasidium pullulans is an extremely oxygen-consuming bioprocess. The utilization of low-cost raw materials, instead of pure glucose or glucose syrup, is a step forward in the development of a sustainable process for GA production. For the first time, sugarcane molasses (ScM), which is approximately 20 times cheaper than pure glucose, was used as the subs...
In recent years, lignocellulosic biomass has become an attractive low-cost raw material for microbial bioprocesses aiming the production of biofuels and other valuable chemicals. However, these feedstocks require preliminary pretreatments to increase their utilization by microorganisms, which may lead to the formation of various compounds (acetic acid, formic acid, furfural, 5-hydroxymethylfurfural, p-coumaric ...
Feedstock costs for the biosynthesis of gluconic acid (GA) represent most of the operating costs at an industrial scale. The increasing demand for GA boosts the search for alternative substrates to pure glucose. The oxidation reaction of glucose to GA, catalyzed by glucose oxidase, is strongly dependent on oxygen availability in the culture medium. The high viscous Aspergillus niger culture, together with the u...
Olive mill wastewaters (OMW), generated during olive oil production, have detrimental effects on the environment due to its high organic load and phenolic compounds content, which have antimicrobial effects. OMW are difficult to be biodegradable, but are a valuable resource of nutrients for microbial growth. In this study, the effect of initial cell concentration, nitrogen supplementation, pH and agitation rate...