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Optimization of heat and ultrasound-assisted extraction of Eucalyptus globulus ...

Lima, Laíres; Pereira, Ana I.; Vaz, Clara B.; Ferreira, Olga; Dias, Maria Inês; Heleno, Sandrina A.; Calhelha, Ricardo C.; Barros, Lillian

The extraction of phenolic compounds from eucalyptus leaves was optimized using heat and ultrasound-assisted techniques, and the bioactive potential of the resulting extract was assessed. The independent variables, including time (t), solvent concentration (S), and temperature (T) or power (P), were incorporated into a fivelevel central composite design combined with Response Surface Methodology. Phenolic conte...

Date: 2025   |   Origin: Biblioteca Digital do IPB

Authenticity and Bioactive Markers Search in the Phenolic‐Rich Extracts of Aste...

Garcia Perez, Pascual; Garcia‐Oliveira, Paula; Finimundy, Tiane C.; Pinela, José; Calhelha, Ricardo C.; Nenadić, Marija; Soković, Marina

The Asteraceae family has been of significant concern for ethnobotanical studies, thanks to its health-promoting properties linked to a plethora of bioactive compounds, among which phenolic compounds play a critical role. In this work, a workflow based on computational chemometrics was employed to assess the authenticity and biomarker search of five key Asteraceae species commonly employed in traditional medici...

Date: 2025   |   Origin: Biblioteca Digital do IPB

Soybean Flour Fortified with Gryllus assimilis Powder to Increase Iron Bioavail...

Barnabé, Michele Lílian da Fonseca; Vicente, Laura Célia de Oliveira Souza; Martins, Karina Vitoria Cipriana; Lacerda, Gabrieli Fernandes

Insects like Gryllus assimilis have an excellent nutritional profile, including iron. However, the bioavailability of this iron and its effects on intestinal health and oxidative balance remain unclear. To enhance acceptance, insects can be used in powder form and combined with common flours. Objective: This study evaluates the effects of Gryllus assimilis powder, alone or with soy flour, on iron bioavailabilit...

Date: 2025   |   Origin: Biblioteca Digital do IPB

Valorisation of pumpkin by-products: Chemical composition and bioactive propert...

Leichtweis, Maria G.; Molina, Adriana K.; Dias, Maria Inês; Calhelha, Ricardo C.; Pires, Tânia C.S.P.; Pavli, Ourania; Oliveira, Beatriz

This study evaluated the fruit by-products (peels, seeds, and fibrous strands) from 11 pumpkin genotypes cultivated in Greece aiming to valorize them as natural sources of bioactive compounds. Five compounds, including ( )-epicatechin and chicoric acid isomers, were identified in peels, while seeds and fibrous strands mainly contained ( )-epicatechin. Organic acids and tocopherols varied significantly among gen...

Date: 2025   |   Origin: Biblioteca Digital do IPB

Valorization of natural resources - development of a functional plant-based bev...

Fernandes, Filipa Alexandra; Roriz, Custódio Lobo; Calhelha, Ricardo C.; Rodrigues, Paula; Pires, Tânia C.S.P.; Prieto Lage, Miguel A.

Plant-based beverages are gaining attention due to their potential to offer sustainable and health-promoting alternatives to traditional dairy products. This study aimed to develop a dehydrated functional plant-based beverage composed of tigernut tubers (Cyperus esculentus L.), mukua pulp (Adansonia digitata L.), and thermal water. All matrices and the final beverage were fully characterized in terms of physica...

Date: 2025   |   Origin: Biblioteca Digital do IPB

Valorization of Solanum melongena L. crop by-products: phenolic composition and...

Añibarro-Ortega, Mikel; Dias, Maria Inês; Petrović, Jovana; Núñez, Sonia; Calhelha, Ricardo C.; Costa, Eduardo M.; Machado, M.; Pintado, Manuela

This study explored the valorization of post-harvest eggplant aerial parts as a sustainable source of value-added ingredients by investigating their phenolic composition and in vitro bioactive properties. HPLC-DAD-ESI/MSn analysis identified chlorogenic acid derivatives as the predominant phenolic compounds (53 % of the phenolic fraction), followed by O-glycosylated kaempferol and quercetin. The extract display...


A biorefinery approach for the simultaneous obtention of essential oils, organi...

Gómez-Mejía, Esther; Dias, Maria Inês; Pereira, Carla; Pires, Tânia C.S.P.; Palá-Paúl, Jesús; Rosales-Conrado, Noelia; León-González, María Eugenia

This investigation evaluates the valorization of citrus peels (lemon, tangerine, and orange) to recover both on-polar and polar fractions simultaneously. Citrus essential oils, abundant in limonene (74.4–33.7 %), exhibited great antioxidant activity (IC50 = 2.002 mg/mL) and Campylobacter jejuni halo inhibition (2.9 cm), particularly in tangerine and orange essential oils. The aqueous extracts were rich in quini...

Date: 2025   |   Origin: Biblioteca Digital do IPB

Acute Effects of Nitrate-Rich Beetroot Juice on Cardiovascular and Hemodynamic ...

Primo, Mateus Chaves; Viana, Ítalo Santiago Alves; Goulart-Silva, Leonardo Silveira; Machado, Wanderson Matheus Lopes; Leite , Luciano Bernardes

Beetroot juice is a popular nutritional resource in sports due to its ergogenic effects, promoting vasodilation, hypotension, improved energy efficiency, and reduced oxygen cost. However, its role in modulating the autonomic nervous system during strength training remains understudied. This study assessed the effects of acute nitrate-rich beetroot juice supplementation on cardiovascular and hemodynamic response...

Date: 2025   |   Origin: Biblioteca Digital do IPB

Optimizing ultrasound-assisted extraction for enhanced yield of bioactive compo...

Plasencia, Paula; Carocho, Márcio; Finimundy, Tiane C.; Calhelha, Ricardo C.; Molina, Adriana K.; Pires, Tânia C.S.P.; Barreiro, Filomena

Due to the high nutritional value of berries, their extensive production has led to the accumulation of bioresidues. To leverage these residues, the distinct chemical composition, and functional properties of the leaves and other aerial parts can be used to produce high-value ingredients for cosmetics. Ultrasound-assisted extraction (UAE) is considered simple, fast, efficient, and more affordable than other tec...

Date: 2025   |   Origin: Biblioteca Digital do IPB

Nutritional and Bioactive Characterization of Unconventional Food Plants for Su...

Oliveira, Izamara; Salgado, José Miguel R.T.; Lopes, João Pedro Krauspenhar; Carocho, Márcio; Silveira, Tayse F. F. da; Garcia, Vitor Augusto dos Santos

Unconventional food plants (UFPs) are increasingly valued for their nutritional composition and bioactive potential. This study proposes a comprehensive characterization of the chemical and bioactive properties of Pereskia aculeata Miller (Cactaceae) (PA); Xanthosoma sagittifolium (L.) Schott (Araceae) (XS); Stachys byzantina K. Koch (Lamiaceae) (SB); and inflorescences from three cultivars of Musa acuminata (M...

Date: 2025   |   Origin: Biblioteca Digital do IPB

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