7 documents found, page 1 of 1
Comparative proteomics of Geobacter sulfurreducens PCAT in response to acetate,...
Mollaei, Monir; Timmers, Peer H. A.; Suarez-Diez, Maria; Boeren, Sjef; van Gelder, Antonie H.; Stams, Alfons Johannes Maria; Plugge, Caroline M.Geobacter sulfurreducens is a model bacterium to study the degradation of organic compounds coupled to the reduction of Fe(III). The response of G. sulfurreducens to the electron donors acetate, formate, hydrogen and a mixture of all three with Fe(III) citrate as electron acceptor was studied using comparative physiological and proteomic approaches. Variations in the supplied electron donors resulted in differe...
Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic aceta...
Timmers, Peer H. A.; Vavourakis, Charlotte D.; Kleerebezem, Robbert; Damste, Jaap S. Sinninghe; Muyzer, Gerard; Stams, Alfons Johannes MariaAnaerobic syntrophic acetate oxidation (SAO) is a thermodynamically unfavorable process involving a syntrophic acetate oxidizing bacterium (SAOB) that forms interspecies electron carriers (IECs). These IECs are consumed by syntrophic partners, typically hydrogenotrophic methanogenic archaea or sulfate reducing bacteria. In this work, the metabolism and occurrence of SAOB at extremely haloalkaline conditions wer...
Evaluation and optimization of PCR primers for selective and quantitative detec...
Timmers, Peer H. A.; Widjaja-Greefkes, H. C. Aura; Plugge, Caroline M.; Stams, Alfons Johannes MariaSince the discovery that anaerobic methanotrophic archaea (ANME) are involved in the anaerobic oxidation of methane coupled to sulfate reduction in marine sediments, different primers and probes specifically targeting the 16S rRNA gene of these archaea have been developed. Microbial investigation of the different ANME subtypes (ANME-1; ANME-2a, b, and c; and ANME-3) was mainly done in sediments where specific s...
Syntrophic acetate oxidation at haloalkaline conditions
Timmers, Peer H. A.; Vavourakis, C.; Muyzer, G.; Sorokin, D.; Stams, Alfons Johannes Maria; Plugge, Caroline M.Syntrophic acetate oxidation (SAO) is a process where syntrophic acetate-oxidizing bacteria (SAOB) convert acetate to form CO2 and reducing equivalents. These reducing equivalents are utilized by a syntrophic partner which in most cases is a hydrogenotrophic methanogen or sulfate-reducing bacterium. Until now, SAO has been found to be dominant over aceticlastic methanogenesis under extreme conditions such as hi...
Anaerobic oxidation of methane associated with sulfate reduction in a natural f...
Timmers, Peer H. A.; Suarez-Zuluaga, Diego A.; Rossem, Minke van; Diender, Martijn; Stams, Alfons Johannes Maria; Plugge, Caroline M.The occurrence of anaerobic oxidation of methane (AOM) and trace methane oxidation (TMO) was investigated in a freshwater natural gas source. Sediment samples were taken and analyzed for potential electron acceptors coupled to AOM. Long-term incubations with 13C-labeled CH4 (13CH4) and different electron acceptors showed that both AOM and TMO occurred. In most conditions, 13C-labeled CO2 (13CO2) simultaneously ...
Growth of anaerobic methane-oxidizing archaea and sulfate reducing bacteria in ...
Timmers, Peer H. A.; Gieteling, Jarno; Widjaja-Greefkes, H. C. Aura; Plugge, C. M.; Stams, Alfons Johannes Maria; Lens, Piet N. L.; Meulepas, Roel J. W.Communities of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria (SRB) grow slowly, which limits the ability to perform physiological studies. High methane partial pressure was previously successfully applied to stimulate growth, but it is not clear how different ANME subtypes and associated SRB are affected by it. Here, we report on the growth of ANME-SRB in a membrane capsule bioreactor...
Growth and activity of ANME clades with different sulfate and sulfide concentra...
Timmers, Peer H. A.; Widjaja-Greefkes, H. C. Aura; Ramiro-Garcia, Javier; Plugge, C. M.; Stams, Alfons Johannes MariaExtensive geochemical data showed that significant methane oxidation activity exists in marine sediments. The organisms responsible for this activity are anaerobic methane-oxidizing archaea (ANME) that occur in consortia with sulfate-reducing bacteria. A distinct zonation of different clades of ANME (ANME-1, ANME-2a/b and ANME-2c) exists in marine sediments, which could be related to the localized concentration...