4 documents found, page 1 of 1
Glucose-induced calcium influx in budding yeast involves a novel calcium transp...
Groppi, Silvia; Belotti, Fiorella; Brandão, Rogélio Lopes; Martegani, Enzo; Tisi, RenataGlucose addition to glucose-starved Saccharomyces cerevisiae cells triggers a quick and transient influx of calcium from the extracellular environment. In yeast at leasttwodifferent carrier systems were identified: a high affinity system, requiring Cch1 channel, and a low affinity system. Here we report that another calcium transport system exists in yeast, not yet identified, that can substitute the two known ...
The PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essen...
Coccetti, Paola; Tisi, Renata; Martegani, Enzo; Teixeira, Leonardo Souza; Brandão, Rogélio Lopes; Castro, Ieso de Miranda; Thevelein, Johan MariaAddition of glucose to glucose-deprived cells of the yeast Saccharomyces cerevisiae triggers rapid turnover of phosphatidylinositol, phosphatidylinositol-phosphate and phosphatidylinositol 4,5-bisphosphate. Glucose stimulation of PI turnover was measured both as an increase in the specific ratio of 32P-labeling and as an increase in the level of diacylglycerol after addition of glucose. Glucose also causes rapi...
The involvement of calcium carriers and of the vacuole in the glucose-induced c...
Bouillet, Leoneide Érica Maduro; Cardoso, Anamaria de Souza; Perovano, Eduardo; Pereira, R. R.; Ribeiro, Erica Milena de CastroPrevious work from our laboratories demonstrated that the sugar-induced activation of plasma membrane H+-ATPase in Saccharomyces cerevisiae is dependent on calcium metabolism with the contribution of calcium influx from external medium. Our results demonstrate that a glucose-induced calcium (GIC) transporter, a new and still unidentified calcium carrier, sensitive to nifedipine and gadolinium and activated by g...
Carbonyl cyanidem - chlorophenylhydrazone induced calcium signaling and activat...
Pereira, Michele B. P.; Tisi, Renata; Fietto, Luciano Gomes; Cardoso, Anamaria de Souza; França, Mônica M.; Carvalho, Fernanda Machado deThe plasma membrane H1-ATPase from Saccharomyces cerevisiae is an enzyme that plays a very important role in the yeast physiology. The addition of protonophores, such as 2,4-dinitrophenol (DNP) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), also triggers a clear in vivo activation of this enzyme. Here, we demonstrate that CCCP-induced activation of the plasma membrane H1-ATPase shares some similarities wi...