4 documents found, page 1 of 1
Antibiofillm activity of propolis extract on Fusarium species from onychomycosis
Galletti, Juliana; Tobaldini-Valerio, Flávia; Silva, Sónia Carina; Kioshima, Erika Seki; Trierveiler-Pereira, Larissa; Bruschi, Marcos; Negri, MelyssaAim: The present study evaluated the capacity of three species of Fusarium isolated from onychomycosis to form bio lms and the antibio lm effect of propolis extract on these bio lms. Materials & methods: The bio lms and antibio lm effects were evaluated by quantifying the colony-forming units, mitochondrial metabolic activity assays, total biomass by crystal violet staining and scanning electron microscopy. Res...
Propolis: a potential natural product to fight Candida species infections
Tobaldini-Valerio, Flávia; Bonfim-Mendonça, P. S.; Rosseto, H. C.; Bruschi, M. L.; Henriques, Mariana; Negri, Melyssa; Silva, Sónia CarinaAim: To evaluate the effect of propolis against Candida species planktonic cells and its counterpart's biofilms. Materials & methods: The MIC values, time-kill curves and filamentation form inhibition were determined in Candida planktonic cells. The effect of propolis on Candida biofilms was assessed through quantification of CFUs. Results: MIC values, ranging from 220 to 880 µg/ml, demonstrated higher efficien...
Discrimination of clinically relevant Candida species by Fourier-transform infr...
Silva, Sónia Carina; Tobaldini-Valerio, Flávia; Costa-de-Oliveira, Sofia; Henriques, Mariana; Azeredo, Joana; Ferreira, Eugénio C.; Lopes, João AlmeidaAccurate Candida species identification remains a challenge due to their phenotypic and genotypic similarity. Species belonging to the *psilosis group, are even described as phenotypically indistinguishable. Also, most of the genotypic methods commonly used to discriminate these species are laborious and very expensive. In this work we developed a Fourier-transform infrared spectroscopy with attenuated total re...
Bioactive properties and functional constituents of Hypericum androsaemum L.: A...
Jabeur, Inés; Tobaldini-Valerio, Flávia; Martins, Natália; Barros, Lillian; Martins, I.; Calhelha, Ricardo C.; Henriques, Mariana; Silva, Sónia CarinaHypericum androsaemum L. ethanol:water extract acted as a lipid peroxidation inhibitor and free radical scavenger. A marked inhibition of the growth of breast, lung, cervical and hepatocellular human carcinoma cell lines was also observed, whereas no toxicity was shown against non-tumor porcine liver cells (> 400 ?g/mL). The extract was also effective in inhibiting nitric oxide production, as an indicator of th...