The cultivation of citrus in the Mediterranean region is of considerable economic importance. The viability of this industry is contingent upon a number of factors, with adequate phytosanitary management being of particular significance. During the last decade, the geographical range of the invasive psyllid, Trioza erytreae (Del Guercio, 1918), has expanded to the mainland territories of Portugal and Spain. Tri...
Dragon fruit (Selenicereus spp.) is a fruit crop with a high commercial value. In recent years, there has been an increased interest in this crop in the southern region of Por tugal, with the introduction of new cultivars (Trindade et al. 2023). In June 2022, a survey was conducted to monitor the sanitary status of a plantation in Moncarapacho. Seven two- to three-year-old plants of Selenicereus stenopterus (F....
A simple protocol to extract RNA from a single wingless aphid, Aphis gossypii and Aphis spiraecola, using liquid nitrogen and Tri-Reagent is described. A nested reverse-transcription polymerase chain reaction with degenerate primers enabled Citrus tristeza virus (CTV) detection in these aphid species collected from an infected tangor ‘Ortanique’ (Citrus × sinensis (L.) Osbeck × Citrus reticulata Blanco) in the ...