4 documents found, page 1 of 1
Near-Infrared Light Activated Formulation for the Spatially Controlled Release ...
Simões, Susana; Lino, Miguel; Barrera, Angela; Rebelo, Catarina; Tomatis, Francesca; Vilaça, Andreia; Breunig, Christopher; Neuner, Andrea; Peça, JoãoThe CRISPR/Cas9 system has emerged as a promising platform for gene editing; however, the lack of an efficient and safe delivery system to introduce it into cells continues to hinder clinical translation. Here, we report a rationally designed gene-editing nanoparticle (NP) formulation for brain applications: an sgRNA:Cas9 ribonucleoprotein complex is immobilized on the NP surface by oligonucleotides that are co...
Gene Editing Nanoformulations for Brain Applications
Tomatis, FrancescaTese de Doutoramento em Biologia Experimental e Biomedicina apresentada ao Instituto de Investigação Interdisciplinar; In response to the increasing global aging population and the associated surge in age-related brain diseases, this work explores innovative approaches to overcome the blood-brain barrier (BBB) and address the neuronal loss in age-related pathologies, such as neurodegenerative disorders and stro...
Engineered extracellular vesicles as brain therapeutics
Lino, Miguel M.; Simões, Susana; Tomatis, Francesca; Albino, Inês; Barrera, Angela; Vivien, Denis; Sobrino, Tomás; Ferreira, LinoExtracellular vesicles (EVs) are communication channels between different cell types in the brain, between the brain and the periphery and vice-versa, playing a fundamental role in physiology and pathology. The evidence that EVs might be able to cross the blood-brain barrier (BBB) make them very promising candidates as nanocarriers to treat brain pathologies. EVs contain a cocktail of bioactive factors, yet the...
Therapeutic Nanoparticles for the Different Phases of Ischemic Stroke
Bernardo-Castro, Sara; Albino, Inês; Barrera-Sandoval, Ángela María; Tomatis, Francesca; Sousa, João André; Martins, Emanuel; Simões, SusanaStroke represents the second leading cause of mortality and morbidity worldwide. Ischemic strokes are the most prevalent type of stroke, and they are characterized by a series of pathological events prompted by an arterial occlusion that leads to a heterogeneous pathophysiological response through different hemodynamic phases, namely the hyperacute, acute, subacute, and chronic phases. Stroke treatment is highl...