12 documents found, page 1 of 2
ESBL and AmpC β-Lactamases in Clinical Strains of Escherichia coli from Serra d...
Oliveira, Cátia Marlene Meneses; Amador, Paula; Prudêncio, C.; Tomaz, C. T.; Ratado, Paulo; Fernandes, RubenBackground and Objectives: Given the considerable spatial, temporal, and ecological factors, heterogeneity, which affects emergency response, persistence, and dissemination of genetic determinants that confer microorganisms their resistance to antibiotics, several authors claim that antibiotics' resistance must be perceived as an ecological problem. The aim of this study was to determine the prevalence of broad...
Impact of cardiac resynchronization therapy on circulating IL-17 producing cell...
Martins, Sílvia; Carvalheiro, Tiago; Laranjeira, Paula; Martinho, António; Elvas, Luís; Gonçalves, Lino; Tomaz, C. T.; António, Natália; Paiva, ArturIL-17-producing T cells have been implicated in the inflammatory milieu of chronic heart failure (CHF), which implies a dismal prognosis in affected patients. The aim of this study was to evaluate the impact of cardiac resynchronization therapy (CRT) on the frequency and functional activity of Th17 and Tc17 cells, as well as, on IL-17 mRNA expression in patients with CHF.
Refinement of two-dimensional electrophoresis for vitreous proteome profiling u...
Santos, Fátima Raquel Milhano dos; Albuquerque, Tânia Gonçalves; Gaspar, Leonor Isabel Mesquita; Dias, Joao ML; Sousa, João Paulo Castro DeDespite technological advances, two-dimensional electrophoresis (2DE) of biological fluids, such as vitreous, remains a major challenge. In this study, artificial neural network was applied to optimize the recovery of vitreous proteins and its detection by 2DE analysis through the combination of several solubilizing agents (CHAPS, Genapol, DTT, IPG buffer), temperature, and total voltage. The highest protein re...
Bacteriocin production of the probiotic Lactobacillus acidophilus KS400
Gaspar, C; Donders, Gilbert; Oliveira, Rita Palmeira de; Queiroz, João; Tomaz, C. T.; Oliveira, J. Martinez de; Oliveira, Ana Palmeira deIn the last years, the use of probiotics, including Lactobacillus species, has received much attention to prevent and treat vaginal disorders. These species have been described as having the ability to colonize the epithelial surface and produce antimicrobial metabolites that are able to control the remaining vaginal microflora. This study aimed to identify and characterize, for the first time, a bacteriocin na...
iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal De...
Santos, F.M.; Gaspar, Leonor Isabel Mesquita; Ciordia, Sergio; Rocha, Ana; Sousa, João Paulo Castro De; Paradela, Alberto; Passarinha, LA; Tomaz, C. T.Rhegmatogenous retinal detachment (RRD) is a potentially blinding condition characterized by a physical separation between neurosensory retina and retinal pigment epithelium. Quantitative proteomics can help to understand the changes that occur at the cellular level during RRD, providing additional information about the molecular mechanisms underlying its pathogenesis. In the present study, iTRAQ labeling was c...
Influenza DNA vaccine purification using pHEMA cryogel support
Santos, Tiago; Brito, Andreia; Boto, Renato; Sousa, Pedro; Almeida, Paulo; Cruz, Carla; Tomaz, C. T.Influenza virus is a huge financial and social burden for health care systems over the world. Currently, traditionalapproaches are not effective in the fight of the epidemy and new alternatives like DNA vaccines have been developed. However, the downstream process of DNA vaccines is a constant challenge in the biotechnology industry. Cryogels has several advantages over traditional supports and have been tested...
Evaluation of the growth factors VEGF-a and VEGF-B in the vitreous and serum of...
Mesquita, Joana; Sousa, João Paulo Castro De; Pereira, Sara Vaz; Neves, Arminda; Passarinha, L A; Tomaz, C. T.VEGF-A and VEGF-B are proangiogenic and key regulating factors for blood vessel growth. This study aims to compare VEGF-A and VEGF-B levels in the serum and vitreous of patients with neovascular pathology versus non-neovascular pathology. Our findings showed vitreous VEGF-A and VEGF-B levels increased in patients with neovascular disease, with higher levels of VEGF-A compared to VEGF-B (p ≤ .05). In the diabeti...
Vascular endothelial growth factors and placenta growth factor in retinal vascu...
Mesquita, Joana; Sousa, João Paulo Castro de; Pereira, Sara Vaz; Neves, Arminda; Passarinha, L A; Tomaz, C. T.Vision loss due to disease or degeneration of the eye (retina, choroid, retinal veins, or macula) is a leading cause of blindness worldwide. In most cases, vision-threatening ocular diseases are accompanied by abnormal changes in the vasculature of the eye, especially the retina, and these conditions are collectively referred to as retinal vasculopathies. Impaired blood supply or hypoxia stimulates angiogenesis...
Plasmid purification by using a new naphthalene tripodal support
Santos, Tiago; Proença, Z.; Queiroz, João; Tomaz, C. T.; Cruz, CarlaThe aim of this work was to employ a new naphthalene tripodal support for the isolation of supercoiled (sc) isoform of plasmid (pDNA) from a native sample. This support is for the first time synthesized and used in pDNA purification. The naphthalene tripodal ligand was synthesized and characterized to assess its purity and subsequently immobilized onto an epoxy-activated Sepharose CL-6B, using mild conditions a...
VEGF-B Levels in the Vitreous of Diabetic and Non-Diabetic Patients with Ocular...
Mesquita, Joana; Sousa, João Paulo Castro De; Pereira, Sara Vaz; Neves, Arminda; Ratado, Paulo; Santos, F.M.; Passarinha, LA; Tomaz, C. T.Vascular endothelial growth factor B (VEGF-B) is one of the enigmatic members of the VEGF family. The knowledge gap about VEGF-B expression and how its levels are altered in diabetic eyes were the focus of this investigation that was addressed by comparing and correlating vitreous VEGF-B between diabetic and non-diabetic patients. VEGF-B levels were measured by enzyme-linked immunosorbent assay in vitreous samp...