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Enhancing the detection of Dinophysis spp. using electrochemical genosensors
Pereira, Eduarda; Silva, Aurora; Morais, Stephanie L.; Costa-Rama, Estefanía; Moreira, Patrícia R.; Fraga-Corral, M.; Torrado, Ana M.Background: Harmful algal blooms (HABs) pose a significant threat to the environment and public health. These blooms are defined by an accumulation of microscopic algae in water, and they can occur in lakes, rivers, estuaries, or coastal areas. Factors like the unregulated runoff of agricultural and industrial wastes into the aquatic environment [1] are believed to have transformed these ecosystems into favorab...
Development of a novel phagomagnetic-assisted isothermal DNA amplification syst...
Maciel, Cláudia; Silva, Nádia F. D.; Teixeira, Paula; Magalhães, Júlia M. C. S.The hitherto implemented Listeria monocytogenes detection techniques are cumbersome or require expensive non-portable instrumentation, hindering their transposition into on-time surveillance systems. The current work proposes a novel integrated system resorting to loop-mediated isothermal amplification (LAMP), assisted by a bacteriophage P100–magnetic platform, coupled to an endpoint electrochemical technique, ...
Enhancing the detection of Dinophysis spp. using electrochemical genosensors
Pereira, Eduarda; Silva, Aurora; Morais, Stephanie L.; Costa-Rama, Estefanía; Moreira, Patrícia R.; Fraga-Corral, M.; Torrado, Ana M.Harmful algal blooms (HABs) pose a significant threat to the environment and public health. These blooms are defined by an accumulation of microscopic algae in water, and they can occur inlakes, rivers, estuaries, orcoastal areas. Factors like the unregulated runoff of agricultural and industrial wastes into the aquatic environment are believed to have transformed these ecosystems into favorable habitats for al...
Is it possible to prevent Harmful Algal Blooms? An electrochemical genosensor t...
Pereira, Eduarda; Silva, Aurora; Morais, Stephanie L.; Costa-Rama, Estefanía; Moreira, Patrícia R.; Fraga-Corral, M.; Torrado, Ana M.Harmful Algal Blooms (HABs) are a very common phenomenon in the last few years that results from the accumulation of microalgae, such as dinoflagellates of the Dinophysis genus. Hence, the development of analytical technologies capable of detecting these microorganisms and thereby avoiding environmental and public health crisis, has become a major priority. Biosensors have been gaining recognition lately given ...
Authenticating honey origin from Natural Park of Montesinho: innovative electro...
Pereira, Eduarda; Morais, Stephanie L.; Seguro, Isabel; Silva, Nádia F. D.; Santos, Marlene; Pacheco, João G.; Delerue-Matos, CristinaThe Natural Park of Montesinho (NPM) has a diverse ecosystem that is home to countless species of trees and plants characteristic of this area, including Castanea sativa. The honey produced in this region is highly appreciated for its taste and nutritional value, but it also has a wide range of health benefits. The geographical origin of the honey is a very important aspect in assessing the quality and authenti...
A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimu...
Silva, Nádia F. D.; Magalhães, Júlia M. C. S.; Oliva-Teles, Teresa; Delerue-Matos, CristinaPotentiometric detection with homemade polymeric membrane microelectrodes was coupled to a magnetic sandwich immunoassay for Salmonella typhimurium determination. Cadmium and sodium ion selective electrodes were used respectively as indicator and pseudo-reference electrodes and were prepared in pipette tips to allow potentiometric measurements in microliter sample volumes. In the proposed method, the concentrat...