13 documents found, page 1 of 2
Evaluation of the ability to form biofilms in KPC-producing and ESBL-producing ...
Sabenca, Carolina; Costa, Eliana; Sousa, Sara; Barros, Lillian; Oliveira, Ana M.P.; Ramos, Sonia; Igrejas, Gilberto; Torres, Carmen; Poeta, PatríciaThe appearance of Klebsiella pneumoniae strains producing extended-spectrum beta-lactamase (ESBL), and carbapenemase (KPC) has turned into a significant public health issue. ESBL- and KPC-producing K. pneumoniae's ability to form biofilms is a significant concern as it can promote the spread of antibiotic resistance and prolong infections in healthcare facilities. A total of 45 K. pneumoniae strains were isolat...
Bioinformatics study of expression from genomes of epidemiologically related MR...
Ribeiro, Miguel; Sousa, Margarida; Borges, Vítor; Gomes, João Paulo; Duarte, Sílvia; Isidro, Joana; Vieira, Luís; Torres, Carmen; Santos, HugoOne of the most important livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) genetic lineages is the clonal complex (CC) 398, which can cause typical S. aureus-associated infections in people. In this work, whole-genome sequencing, RNA-sequencing, and gel-based comparative proteomics were applied to study the genetic characteristics of three MRSA CC398 isolates recovered from humans (str...
MRSA CC398 recovered from wild boar harboring new SCCmec type IV J3 variant
Sousa, Margarida; Silva, Nuno; Borges, Vítor; Gomes, João P.; Vieira, Luís; Caniça, Manuela; Torres, Carmen; Igrejas, Gilberto; Poeta, PatríciaA methicillin-resistant Staphylococcus aureus CC398 was recovered from a wild female boar (Sus scrofa) in the north of Portugal, in 2013 (Sousa et al. 2017). Whole genome sequencing (WGS) revealed this strain carries a new variant of a mecA-containing staphylococcal chromosomal gene cassette (SCCmec) type IV with an uncommon J3 region. WGS studies can facilitate surveillance and provide more detailed characteri...
Multiomics Substrates of Resistance to Emerging Pathogens? Transcriptome and Pr...
Pinto, Luís; Torres, Carmen; Gil, Concha; Santos, Hugo M.; Capelo, José Luís; Borges, Vítor; Gomes, João Paulo; Silva, Catarina; Vieira, LuísAntibiotic resistance and hospital acquired infections are on the rise worldwide. Vancomycin-resistant enterococci have been reported in clinical settings in recent decades. In this multiomics study, we provide comprehensive proteomic and transcriptomic analyses of a vancomycin-resistant Enterococcus faecalis clinical isolate from a patient with a urinary tract infection. The previous genotypic profile of the s...
Multiomicssubstrates of resistance to emerging pathogens? transcriptome and pro...
Pinto, Luís; Torres, Carmen; Gil, Concha; Santos, Hugo M.; Capelo, José Luís; Borges, Vítor; Gomes, João Paulo; Silva, Catarina; Vieira, LuísAntibiotic resistance and hospital acquired infections are on the rise worldwide. Vancomycin-resistant enterococci have been reported in clinical settings in recent decades. In this multiomics study, we provide comprehensive proteomic and transcriptomic analyses of a vancomycin-resistant Enterococcus faecalis clinical isolate from a patient with a urinary tract infection. The previous genotypic profile of the s...
Multiomics Assessment of Gene Expression in a Clinical Strain of CTX-M-15-Produ...
Pinto, Luís; Torres, Carmen; Gil, Concha; Nunes-Miranda, Júlio D.; Santos, Hugo M.; Borges, Vítor; Gomes, João P.; Silva, Catarina; Vieira, LuísExtended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strain C999 was isolated of a Spanish patient with urinary tract infection. Previous genotyping indicated that this strain presented a multidrug-resistance phenotype and carried beta-lactamase genes encoding CTX-M-15, TEM-1, and OXA-1 enzymes. The whole-cell proteome, and the membrane, cytoplasmic, periplasmic and extracellular sub-proteomes of ...
Planning a One Health Case Study to Evaluate Methicillin Resistant Staphylococc...
Igrejas, Gilberto; Correia, Susana; Silva, Vanessa; Hébraud, Michel; Caniça, Manuela; Torres, Carmen; Gomes, Catarina; Nogueira, FernandaMethicillin-resistant Staphylococcus aureus (MRSA) is one of the most important multidrug-resistant nosocomial pathogens worldwide with infections leading to high rates of morbidity and mortality, a significant burden to human and veterinary clinical practices. The ability of S. aureus colonies to form biofilms on biotic and abiotic surfaces contributes further to its high antimicrobial resistance (AMR) rates a...
Chemical composition, antioxidant and antimicrobial activity of phenolic compou...
Silva, Vanessa; Igrejas, Gilberto; Falco, Virgílio; Santos, Teresa P.; Torres, Carmen; Oliveira, Ana M.P.; Pereira, José Eduardo; Amaral, Joana S.Winery by-products are a rich source of polyphenols, which have proven to have several beneficial biological properties, such as, antioxidant and antimicrobial activities. Therefore, this study aimed the extraction of polyphenols from winery by-products of two Portuguese red grape varieties, Touriga Nacional and Preto Martinho, and evaluate their phenolic profile, antioxidant properties and antimicrobial activi...
First report on MRSA CC398 recovered from wild boars in the north of Portugal. ...
Sousa, Margarida; Silva, Nuno; Manageiro, Vera; Ramos, Sónia; Coelho, António; Gonçalves, David; Caniça, Manuela; Torres, Carmen; Igrejas, GilbertoThe aim of the present study was to evaluate the resistance of Staphylococcus aureus recovered from wild boars, to analyze their genetic lineages, and to investigate the susceptibility to oxacillin. Samples from mouth and nose of 45 wild boars (Sus scrofa) were collected during hunt activity from November 2012 to January 2013 in the North of Portugal. S. aureus isolates were recovered from 30 of these samples (...
Subproteomic signature comparison of in vitro selected fluoroquinolone resistan...
Correia, Susana; Hébraud, Michel; Chafsey, Ingrid; Chambon, Christophe; Viala, Didier; Torres, Carmen; Caniça, Manuela; Capelo, José LuisBackground: Fluoroquinolone resistance in nontyphoidal Salmonella is a situation of serious and international concern, particularly in S. Typhimurium DT104B multiresistant strains. Although known to be multifactorial, fluoroquinolone resistance is still far from a complete understanding. Methods: Subproteome changes between an experimentally selected fluoroquinolone-resistant strain (Se6-M) and its parent strai...