6 documents found, page 1 of 1
Microwave-assisted extraction of raspberry pomace phenolic compounds, and their...
Jaouhari, Yassine; Bordiga, Matteo; Travaglia, Fabiano; Coisson, Jean-Daniel; Barbosa, Augusto Alexandre Costa; Sampaio, Paula; Botelho, C. M.Raspberry pomace (RBP) is rich in phenolic compounds. This study aims to optimize the extraction of phenolics from RBP and assess their bioaccessibility and bioactivity. The extraction process revealed that ethanol 50% was the most effective solvent. Microwave-assisted hydroethanolic extraction (MAE) significantly outperformed conventional methods, with optimal conditions of 200°C for 10min yielding the highest...
Candida albicans chitinase 3 with potential as a vaccine antigen: production, p...
Barbosa, Augusto Alexandre Costa; Ferreira, Diogo; Pacheco, Maria Inês; Casal, Margarida; Duarte, Henrique Oliveira; Gomes, CatarinaChitinases are widely studied enzymes that have already found widespread application. Their continued development and valorisation will be driven by the identification of new and improved variants and/or novel applications bringing benefits to industry and society. We previously identified a novel application for chitinases wherein the Candida albicans cell wall surface chitinase 3 (Cht3) was shown to have pote...
New generation antigen delivery system for Candida albicans recombinant protein...
Barbosa, Augusto Alexandre CostaOver the past five decades, a rise in numbers of immunocompromised individuals has led to a significant increase in invasive fungal infections, including those caused by Candida albicans. This has created a pressing need for innovative approaches to combat these fungal pathogens. While several immunotherapeutic strategies and vaccines are in development, none have reached clinical use yet. Previous work emphasi...
Yeast as a Biotechnological factory
Rendulić, Tony; Barbosa, Augusto Alexandre Costa; Pereira, Cátia; Barata, Cláudia; Mendes, Filipa; Pereira, Humberto; Ferreira, João CarlosFor thousands of years, yeast has been used for making beer, wine and bread. Production of these commodities relies on yeasts natural ability to rapidly consume sugars and convert them into carbon dioxide and alcohol. Mostly due to tremendous progress in genetic engineering during the past 50 years, yeast is nowadays also used for production of biofuels, pharmaceuticals and chemicals. It can even be engineered ...
High variability within Candida albicans transcription factor RLM1: Isolates fr...
Pereira, Joana Isabel Carvalho; Oliveira, Manoel M. Evangelista; Barbosa, Augusto Alexandre Costa; Vaz, C.; Velegraki, A.; Zancopé-Oliveira, R. M.Previous studies have correlated the severity of recurrent vulvovaginal Candida infections (VVC) and balanitis in patients from China with the presence of some dominant genotypes at the ORF RLM1. Here we tested VVC vs non-VVC isolates from Portugal, Brazil and Greece and, although the same genotypes were identified in VVC isolates, they were present in only five out of 150 strains. However, this analysis showed...
Evaluation of cell activation promoted by tantalum and tantalum oxide coatings ...
Moreira, Hugo; Barbosa, Augusto Alexandre Costa; Marques, Sandra Mariana Silva; Sampaio, Paula; Carvalho, S.Previous studies have shown both Ta and TaeO to be bioactive, rapidly forming a strongly-bonded surfaceadherent layer of bone-like hydroxyapatite (HAp) when immersed in simulated body fluid (SBF). Consequently, Ta and TaeO coatings are promising for the surface-modification of Ti or stainless steel endodontic endosseous implants, being conducive to a reduction in the risk of developing post-operatory infection ...