The largest endemic areas of paracoccidioidomycosis (PCM) in Brazil comprise the humid agricultural regions of the Southeast, South, and, recently, the Midwest and North regions. The Ceara State, located in the Brazilian Northeast region, presents semi-arid climate in most of its territory, characterized by high temperatures, scarce vegetation and low humidity. The objective of the present study was to describe...
Azole-resistant Candida albicans have been described in human clinical practice and in strains of veterinary origin. The objective of this study was to reevaluate laboratory identification of azole-resistantC. albicansfrom veterinary isolates, proposing a combination of phenotypic methods for the reliable identification of the species. In addition, this studyaimed to investigate the resistance mechanisms of the...
This study investigated potential mechanisms of azole resistance among Candida albicans from animals, including efflux pump activity, ergosterol content and gene expression. For this purpose, 30 azole-resistant C. albicans strains from animals were tested for their antifungal susceptibility, according to document M27-A3, efflux pump activity by rhodamine 6G test, ergosterol content and expression of the genes C...
This study aimed to investigate the influence of tetraconazole and malathion, both used in agricultural activities, on resistance to fluconazole, itraconazole and voriconazole in Candida parapsilosis ATCC 22019. The susceptibility to tetraconazole, malathion, fluconazole, itraconazole and voriconazole, through broth microdilution. Then, 12 independent replicates, were separated and exposed to four treatment gro...
The present study evaluated the maintenance of Sporothrix spp. (6 Sporothrix brasiliensis;6S. schenckii;5 S. mexicana, and 3 S. globosa) in saline at 4 C, and in 10% glycerol plus either 10% lactose or 10% sucrose, at -20 C and -80 C. Viability was assessed after 3, 6, and 9 months of storage, through the recovery of strains on potato dextrose agar and analysis of macro- and micromorphological features. Conidiu...
Dermatophytosis is the most common skin infectious disturbance in the world. In this research 2.297 patients were evaluated with suspected clinical lesions of dermatophytosis. It was observed that, 534 (23.2%) patients tested positive for dermatophytes. T. rubrum was the most prevalent specie (49.6%; p ≤ 0.05), followed by T. tonsurans (34.4%), M. canis (7%) and T. mentagrophytes (6.2%). When the species isolat...
Coccidiodomycosis is a disease caused by dimorphic fungi of the Coccidioides genus – C. immitis and C. posadasii – which afflicts people and various animals, such as cattle, goats, rodents and dogs. It is restricted to the Americas and cases have been reported in the state of Ceará only since 1995. The infection generally results from inhaling the infectious structures arthroconidia -of the fungus. In its paras...