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Design of Artificial Enzymes Using the Metals of the Periodic Table

Nitric Oxide Detection Using Electrochemical Third-generation Biosensors - Base...

Nitric oxide radical (NO) is a signalling molecule involved in virtually all forms of life. Its relevance has been leading to the development of different analytical methodologies to assess the temporal and spatial fluxes of NO under the complex biological milieu. Third‐generation electrochemical biosensors are promising tools for in loco and in vivo NO quantification and, over the past years, heme proteins and...

SERR spectroelectrochemical study of cytochrome cd1 nitrite reductase co-immobi...

"Cytochrome cd1 nitrite reductases (cd1NiRs) catalyze the one-electron reduction of nitrite to nitric oxide. Due to their catalytic reaction, cd1NiRs are regarded as promising components for biosensing, bioremediation and biotechnological applications. Motivated by earlier findings that catalytic activity of cd1NiR from Marinobacter hydrocarbonoclasticus (Mhcd1) depends on the presence of its physiological redo...

Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by ...

The activity of sulfate-reducing bacteria (SRB) intensifies the problems associated to corrosion of metals and the solution entails significant economic costs. Although molybdate can be used to control the negative effects of these organisms, the mechanisms triggered in the cells exposed to Mo-excess are poorly understood. In this work, the effects of molybdate ions on the growth and morphology of the SRB Desul...

Respiratory versatility in Desulfovibrio desulfuricans ATCC 27774 – a proteomic...

Probing the surface chemistry of different oxidized MWCNT for the improved elec...

"This work reports the evaluation of a set of multi-walled carbon nanotubes (MWCNT) presenting different surface chemistries, as interfaces for the direct electrochemistry of the multihemic nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC27774 (Dd). The carbon nanotubes dispersions were prepared in aqueous media and deposited on pyrolytic graphite (PG) macroelectrodes, following a layer-by-layer ...

Recent advances into vanadyl, vanadate and decavanadate interactions with actin

Metallomics. 2012 Jan 3;4(1):16-22

Recent advances into vanadyl, vanadate and decavanadate interactions with actin

Although the number of papers about ‘‘vanadium’’ has doubled in the last decade, the studies about ‘‘vanadium and actin’’ are scarce. In the present review, the effects of vanadyl, vanadate and decavanadate on actin structure and function are compared. Decavanadate 51V NMR signals, at 516 ppm, broadened and decreased in intensity upon actin titration, whereas no effects were observed for vanadate monomers, at 5...

Study of membrane ageing and grafting mechanisms using electron paramagnetic re...

An important setback for a wider use of membrane processes in industry is fouling, caused by aggregation of biomolecules at membrane surface and pores. Two important approaches to reduce this effect are the use of chemical cleaning procedures and the functionalisation of the membrane surface. However, both processes may lead to membrane degradation and structure alteration due to free radical formation or radic...

Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rub...

Inorganic Chemistry 50(21):10600-7; Two cyclen-derived Gd probes, [Gd-DOTAM](3+) and [Gd-DOTP](5-) (DOTAM = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetamide; DOTP = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methylenephosphonate)), were assessed as paramagnetic relaxation enhancement (PRE)-inducing probes for characterization of protein-protein interactions. Two proteins, Desulfovibrio gigas rubredo...