3 documents found, page 1 of 1
Thermostability improvement of Orpinomyces sp. xylanase by directed evolution
Trevizano, Larissa Mattos; Ventorim, Rafaela Zandonade; Rezende, Sebastião Tavares de; Silva Junior, Floriano Paes; Guimarães, Valéria MontezeThe methodology of directed evolution, using the mutagenic technique of error-prone PCR has been used to improve the thermostability of enzymes. This method was applied to the endo-β-1,4-xylanase from Orpinomyces strain PC-2. The constructed library of xylanase (xynA) mutants was subjected to several screening cycles in plates with azo-xylan-agarose as substrate and four thermostable mutants (M1–M4) were select...
Impact of the removal of N-terminal non-structured amino acids on activity and ...
Ventorim, Rafaela Zandonade; Mendes, Tiago Antônio de Oliveira; Trevizano, Larissa Mattos; Camargos, Ana Maria dos Santos; Guimarães, Valéria MontezeXylanases catalyze the random hydrolysis of xylan backbone from plant biomass and thus, they have application in the production of biofuels, Kraft pulps biobleaching and feed industry. Here, xylanases derived from Orpinomyces sp. PC-2 were engineered guided by molecular dynamics methods to obtain more thermostable enzymes. Based on these models, 27 amino acid residues from the N-terminal were predicted to reduc...
Melhoramento por evolução dirigida da termoestabilidade da xilanase xynA de Orp...
Trevizano, Larissa MattosCoordenação de Aperfeiçoamento de Pessoal de Nível Superior; Xylan is the most common hemicellulosic polysaccharide in cell walls of land plants. This polysaccharide is composed of a main-chain polymer made up exclusively of β-1,4 xylose residues. This backbone possesses branches containing arabinofuranosyl, acetyl, and glucuronosyl residues. Enzymatic hydrolysis of xylans is brought about by a variety of ...