7 documents found, page 1 of 1
Maternal thyroid hormone is required to develop the hindbrain vasculature in ze...
Trindade, Marlene; Silva, Nádia; Rodrigues, Joana; Kawakami, Koichi; Campinho, Marco AntónioThyroid hormone (TH) signaling is important and necessary for proper neurodevelopment. Inadequate levels of maternally derived THs (MTH) supply affect target gene expression profiles, which are fundamental for the brain’s normal growth, maturation, and function. The monocarboxylate transporter 8 (SLC16A2, MCT8) is the main TH transporter present in the brain during embryonic development, and mutations in this t...
The xenobiotic sensor PXR in a marine flatfish species (Solea senegalensis): Ge...
Marques, Carlos; Roberto, Vania Palma; Granadeiro, Luis; Trindade, Marlene; Gavaia, Paulo; Laizé, Vincent; Leonor Cancela, M.; Fernandez, IgnacioThe pregnane X receptor (PXR) is a nuclear receptor belonging to the NR1I sub-family and a known master regulator of xenobiotic metabolism. New roles have been recently proposed in mammals through its activation by vitamin K (VK) such as regulation of glucose metabolism, bone homeostasis, reproduction, neuronal development and cognitive capacities. In marine fish species little is known about PXR and its potent...
Transcriptomics reveal an integrative role for maternal thyroid hormones during...
Silva, Nadia; Louro, Bruno; Trindade, Marlene; Power, Deborah M.; Campinho, Marco A.Thyroid hormones (THs) are essential for embryonic brain development but the genetic mechanisms involved in the action of maternal THs (MTHs) are still largely unknown. As the basis for understanding the underlying genetic mechanisms of MTHs regulation we used an established zebrafish monocarboxylic acid transporter 8 (MCT8) knock-down model and characterised the transcriptome in 25hpf zebrafish embryos. Subseq...
Acute Loss of Cited2 Impairs Nanog Expression and Decreases Self-Renewal of Mou...
Kranc, Kamil R.; Oliveira, Daniel; Armesilla-Diaz, Alejandro; Pacheco-Leyva, Ivette; Matias, Ana Catarina; Escapa, Ana Luísa; Subramani, ChithraIdentifying novel players of the pluripotency gene regulatory network centered on Oct4, Sox2, and Nanog as well as delineating the interactions within the complex network is key to understanding self-renewal and early cell fate commitment of embryonic stem cells (ESC). While overexpression of the transcriptional regulator Cited2 sustains ESC pluripotency, its role in ESC functions remains unclear. Here, we show...
Unravelling the evolution of the Allatostatin-Type A, KISS and Galanin Peptide-...
Felix, Rute C.; Trindade, Marlene; Pires, Isa R. P.; Vera G Fonseca; Martins, Rute S.; Silveira, Henrique; Power, Deborah M.; Cardoso, João CRAllatostatin type A receptors (AST-ARs) are a group of G-protein coupled receptors activated by members of the FGL-amide (AST-A) peptide family that inhibit food intake and development in arthropods. Despite their physiological importance the evolution of the AST-A system is poorly described and relatively few receptors have been isolated and functionally characterised in insects. The present study provides a c...
Development of an in vitro cell system from zebrafish suitable to study bone ce...
Vijayakumar, Parameswaran; Laizé, Vincent; Cardeira Da Silva, João; Trindade, Marlene; Cancela, M. LeonorMechanisms of bone formation and skeletal development have been successfully investigated in zebrafish using a variety of in vivo approaches, but in vitro studies have been hindered due to a lack of homologous cell lines capable of producing an extracellular matrix (ECM) suitable for mineral deposition. Here we describe the development and characterization of a new cell line termed ZFB1, derived from zebrafish ...
Estudo da interacção do decavanadato com bicamadas lipídicas
Trindade, MarleneO vanádio é um metal de transição de grande interesse nas áreas da química, bioquímica, bem como das ciências da vida e da saúde, em consequência de se terem descoberto acções benéficas dos compostos contendo vanádio, com acção anti-diabética e anti-cancerígena, a realçar de entre outros efeitos farmacológicos. A espécie decamérica do vanádio, também designada decavanadato, tem sido alvo de muitos estudos no de...