7 documents found, page 1 of 1
Internalization studies on zeolite nanoparticles using human cells
Vilaça, Natália; Totovao, Ricardo; Prasetyanto, Eko Adi; Miranda-Goncalves, Vera; Morais-Santos, Filipa; Fernandes, Rui; Figueiredo, FranciscoZeolites are crystalline porous materials with a regular framework which have non-toxic effects on a variety of human cell lines and have been explored for cell imaging and drug delivery. Understanding the interaction between zeolite nanoparticles and cells is imperative for improving their potentialities, since the process of internalization of these particles is still poorly understood. In this study, the int...
Comparison of different silica microporous structures as drug delivery systems ...
Vilaca, Natalia; Machado, Ana F.; Morais-Santos, Filipa; Amorim, Ricardo; Neto, A. Patricia; Logodin, Enora; Pereira, Manuel F. R.; Sardo, MarianaSeveral silica microporous structures have been studied for their potential as drug delivery systems (DDS) over the last years. However, systematic studies comparing host structures with different topologies and particle sizes, and toxicity studies to human cancer cells, are scarce. In the present work, 3D crystalline structures, three different zeolites (large, medium and small pore size) and one titanosilicat...
Targeting lactate transport suppresses in vivo breast tumour growth
Morais-Santos, Filipa; Granja, Sara; Miranda-Gonçalves, Vera; Moreira, António H.J.; Queirós, Sandro; Vilaça, João L.; Schmitt, Fernando C.Background: Most cancers, including breast cancer, have high rates of glucose consumption, associated with lactate production, a process referred as “Warburg effect”. Acidification of the tumour microenvironment by lactate extrusion, performed by lactate transporters (MCTs), is associated with higher cell proliferation, migration, invasion, angiogenesis and increased cell survival. Previously, we have described...
Reprogramming energy metabolism and inducing angiogenesis: co-expression of mon...
Pinheiro, Céline; Garcia, Eduardo A.; Morais-Santos, Filipa; Moreira, Marise A. R.; Almeida, Fábio M.; Jubé, Luiz F.; Queiroz, Geraldo S.Background: Deregulation of cellular energetic metabolism was recently pointed out as a hallmark of cancer cells. This deregulation involves a metabolic reprogramming that leads to a high production of lactate. Lactate efflux, besides contributing for the glycolytic flux, also acts in the extracellular matrix, contributing for cancer malignancy, by, among other effects, induction of angiogenesis. However, studi...
Micro- and Mesoporous Structures as Drug Delivery Carriers for Salicylic Acid
Vilaca, Natalia; Morais-Santos, Filipa; Machado, Ana F.; Sirkecioglu, Ahmet; Pereira, Manuel F. R.; Sardo, Mariana; Rocha, Joao; Parpot, PierThe potential of salicylic acid (SA) encapsulated in porous materials as drug delivery carriers for cancer treatment was studied. Different porous structures, the microporous zeolite NaY, and the mesoporous SBA-15 and MCM-41 were used as hosts for the anti-inflammatory drug. Characterization with different techniques (FTIR, UV/vis, TGA, H-1 NMR, and C-13 CPMAS NMR) demonstrated the successful loading of SA into...
Characterization of monocarboxylate transporters (MCTs) expression in soft tiss...
Pinheiro, Celine; Penna, Valter; Morais-Santos, Filipa; Abrahao-Machado, Lucas F.; Ribeiro, Guilherme; Curcelli, Emilio Carlos [UNESP]Made available in DSpace on 2014-12-03T13:08:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-05-09Bitstream added on 2014-12-03T13:23:21Z : No. of bitstreams: 1 WOS000336931400001.pdf: 1483231 bytes, checksum: d44026d999cd265cc444aeb241af66a4 (MD5); FCT (Portuguese Foundation for Science and Technology); FCT; Background: Soft tissue sarcomas (STSs) are a group of neoplasms, which, despite current ther...
TCTEX1D4 interactome in human testis: unraveling the function of dynein light c...
Freitas, Maria João; Korrodi-Gregório, Luís; Morais-Santos, Filipa; Silva, Edgar da Cruz e; Fardilha, MargaridaStudies were designed to identify the TCTEX1D4 interactome in human testis, with the purpose of unraveling putative protein complexes essential to male reproduction and thus novel TCTEX1D4 functions. TCTEX1D4 is a dynein light chain that belongs to the DYNT1/TCTEX1 family. In spermatozoa, it appears to be important to sperm motility, intraflagellar transport, and acrosome reaction. To contribute to the knowledg...