53 documents found, page 1 of 6
Engineering Saccharomyces cerevisiae for the one-step production of a functiona...
Amorim, Cláudia; Rodrigues, Joana Lúcia Lima Correia; Braga, Adelaide; Gomes, Daniela Filipa Correia; Costa, João Manuel RainhaFructooligosaccharides (FOS) are recognized prebiotics with proven beneficial effects, usually produced by two-step processes. In this work, an in vivo bioprocess was developed for the one-step production of a functional sweetening mixture containing FOS. Sucrose was directly fermented by yeast towards process simplification and cost reduction. Saccharomyces cerevisiae YIL162W lacking the gene responsible for s...
Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4
Braga, Adelaide; Gomes, Daniela Filipa Correia; Costa, João Manuel Rainha; Cardoso, Beatriz; Amorim, Cláudia; Silvério, Sara Isabel CruzThe online version contains supplementary material available at https://doi.org/10.1007/s00253-022-12037-3; Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, reco...
Microbial production of flavors and fragrances by Yarrowia lipolytica
Braga, Adelaide; Belo, IsabelThe consumers' demand for natural flavors and fragrances has resulted in a decrease in natural resources, making the development of cost-effective biotechnological processes that fulfill consumer needs. The extraction of these compounds from natural sources and chemical synthesis are the most common methods for their production. However, plant extraction has a number of drawbacks, including the low concentratio...
Zymomonas mobilis as an emerging biotechnological chassis for the production of...
Braga, Adelaide; Gomes, Daniela; Costa, João Manuel Rainha; Amorim, Cláudia; Cardoso, Beatriz; Gudiña, Eduardo José; Silvério, Sara Isabel CruzZymomonas mobilis is a well-recognized ethanologenic bacterium with outstanding characteristics which make it a promising platform for the biotechnological production of relevant building blocks and fine chemicals compounds. In the last years, research has been focused on the physiological, genetic, and metabolic engineering strategies aiming at expanding Z. mobilis ability to metabolize lignocellulosic substra...
Engineering Zymomonas mobilis to produce tailor-made fructooligosaccharides
Braga, Adelaide; Gomes, Daniela; Amorim, Cláudia; Cardoso, B.; Silvério, Sara Isabel Cruz; Rodrigues, Joana Lúcia Lima Correia; Rodrigues, L. R.[Excerpt] Background: Fructooligosaccharides (FOS) are promising prebiotics in the increasing market of functional food. Industrially, these compounds are produced from sucrose using fructosyltransferase (Ftase) or β-fructofuranosidase enzymes (Ffase) [1]. Although it is known that these compounds may have a great impact in health, it has also been described that the beneficial effects may depend on the relativ...
Zymomonas mobilis: a promising microorganism for prebiotic production
Braga, Adelaide; Gomes, Daniela; Rainha, João; Amorim, Cláudia Catarina Oliveira; Cardoso, B.; Gudiña, Eduardo José; Silvério, Sara Isabel CruzZymomonas mobilis (ZM) is an ethanologenic bacterium with outstanding characteristics which makes it an interesting chassis for the biotechnological production of prebiotics. Fructooligosaccharides (FOS) are promising prebiotics in the increasing market of functional food. In this work, a Box-Behnken design approach was used to optimize the medium composition and maximize the FOS content. Under optimal conditio...
Valorization of crude glycerol as carbon source for the bioconversion of L-phen...
Braga, Adelaide; Freitas, Bruna; Cordeiro, Ana; Belo, IsabelAccepted article; BACKGROUND 2-Phenylethanol (2-PE) is an aromatic alcohol with delicate fragrance of rose petals, widely used in perfumes, cosmetics, foods, beverages, and pharmaceutical industries. The yeast Yarrowia can produce 2-PE through L-Phenylalanine (L-Phe) biotransformation. However, it is poorly explored in this field, despite its interesting characteristics for this process, such as the Crabtree ne...
Modification of PET surfaces with Gum Arabic towards its bacterial anti-adhesiv...
Caykara, Tugce; Silva, José; Fernandes, Sara; Braga, Adelaide; Rodrigues, Joana Lúcia Lima Correia; Rodrigues, L. R.; Silva, CarlaBacterial adhesion onto hospital material surfaces still represents a big healthcare issue, being preventive measures required to mitigate this problem, such as increasing material surface hydrophilicity. In the present study, gum Arabic, a hydrophilic polysaccharide, was used to modify the surface of polyethylene terephthalate (PET). Initial water contact angle (WCA) and WCA after several washing cycles were s...
Recombinant Saccharomyces cerevisiae as a microbial biocatalyst for the one-ste...
Amorim, Cláudia; Braga, Adelaide; Rodrigues, Joana Lúcia Lima Correia; Cardoso, Beatriz A. Batista; Rainha, João; Gudiña, Eduardo JoséFructooligosaccharides (FOS) are widely consumed prebiotics with proven beneficial effects on both human and animal health. As a result, alternative production processes with high-efficiency have been an increasing focus of interest by both academy and industry. In this work, a in vivo bioprocess approach was successfully developed for one-step production of FOS from sucrose fermentation by recombinant yeast. S...
Collagen-coated magnetic nanoparticles to capture pathogens from biological sam...
Sande, Maria; Roque, Lúcia; Marques, Márcia; Ferreira, Débora; Rodrigues, Joana Lúcia Lima Correia; Braga, Adelaide; Ramada, David; Silva, C.Conventional methods of diagnosing bacterial infections such as microbial culture and molecular techniques, while highly sensitive, rely on expensive equipment and highly skilled operators. There is a need for affordable and portable diagnostic systems which are simple to operate while preserving reliability. Pre-enrichment of bacteria present in a sample coupled with subsequent bacterial identification steps c...