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Expression in Escherichia coli of cysteine protease inhibitors from cowpea (Vig...
Monteiro Júnior, José Edvar; Valadares, Napoleão Fonseca; Pereira, Humberto D’Muniz; Dyszy, Fábio Henrique; Costa Filho, Antônio José daTwo cysteine proteinase inhibitors from cowpea, VuCys1 and VuCys2, were produced in E. coli ArcticExpress (DE3). The recombinant products strongly inhibited papain and chymopapain as well as the midgut proteases from Callosobruchus maculatus larvae, a bruchid that uses cysteine proteases as major digestive enzymes. Heat treatment at 100 °C for up to 60 min or incubation at various pH values caused little reduct...
Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism prof...
Maria, Roberta M.; Altei, Wanessa F.; Valadares, Napoleão Fonseca; Garratt, Richard C.; Andricopulo, Adriano D.; Venâncio, Tiago; Colnago, Luiz A.Conjugated linoleic acid (CLA), a fatty acid found in ruminant food products, has been associated with anticarcinogenic activity. However, its effect on cancer metabolism is unclear. In this paper we evaluated the effects of cis-9, trans-11 CLA on the metabolic profile of MCF-7 and MDA-MB-231 breast cancer cells using high-resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy. T...