15 documents found, page 1 of 2
16S Genomics for Diagnosing Invasive Bacterial Infection
Vale, Filipa; Tanoeiro, Luis; Marques, Andreia T.Invasive bacterial infections (IBI) are leading causes of global morbidity and mortality. Successful treatment of the patients depends on a rapid and accurate diagnosis. The current golden standard procedure for bacterial identification in clinical context requires bacterial culture and biochemical analysis—a slow, culture-dependent and possible subjective approach, presenting several limitations. Bacteria iden...
Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli ...
Tanoeiro, Luis; Oleastro, Mónica; Nunes, Alexandra; Marques, Andreia T.; Duarte, Sílvia Vaz; Gomes, João Paulo; Matos, António Pedro AlvesCampylobacter coli and C. jejuni, the causing agents of campylobacteriosis, are described to be undergoing introgression events, i.e., the transference of genetic material between different species, with some isolates sharing almost a quarter of its genome. The participation of phages in introgression events and consequent impact on host ecology and evolution remain elusive. Three distinct prophages, named C. j...
Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli ...
Tanoeiro, Luis; Oleastro, Mónica; Nunes, Alexandra; Marques, Andreia T.; Duarte, Sílvia Vaz; Gomes, João Paulo; Matos, António Pedro AlvesCampylobacter coli and C. jejuni, the causing agents of campylobacteriosis, are described to be undergoing introgression events, i.e., the transference of genetic material between different species, with some isolates sharing almost a quarter of its genome. The participation of phages in introgression events and consequent impact on host ecology and evolution remain elusive. Three distinct prophages, named C. j...
Genomic Analysis of Prophages from Klebsiella pneumoniae Clinical Isolates
Marques, Andreia T.; Tanoeiro, Luis; Duarte, Aida; Gonçalves, Luisa; Vítor, Jorge M. B.; Vale, FilipaKlebsiella pneumoniae is an increasing threat to public health and represents one of the most concerning pathogens involved in life-threatening infections. The resistant and virulence determinants are coded by mobile genetic elements which can easily spread between bacteria populations and co-evolve with its genomic host. In this study, we present the full genomic sequences, insertion sites and phylogenetic ana...
Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to ...
Marques, Andreia T.; Vítor, Jorge M. B.; Santos, Andrea; Oleastro, Mónica; Vale, FilipaFor a long time Helicobacter pylori infections have been treated using the macrolide antibiotic, clarithromycin. Clarithromycin resistance is increasing worldwide and is the most common cause of H. pylori treatment failure. Here we review the mechanisms of antibiotic resistance to clarithromycin, detailing the individual and combinations of point mutations found in the 23S rRNA gene associated with resistance. ...
A 500-year tale of co-evolution, adaptation, and virulence: Helicobacter pylori...
Muñoz-Ramirez, Zilia Y.; Pascoe, Ben; Mendez-Tenori, Alfonso; Mourkas, Evangelos; Sandoval-Motta, Santiago; Perez-Perez, Guillermo; Morgan, Douglas R.Helicobacter pylori is a common component of the human stomach microbiota, possibly dating back to the speciation of Homo sapiens. A history of pathogen evolution in allopatry has led to the development of genetically distinct H. pylori subpopulations, associated with different human populations, and more recent admixture among H. pylori subpopulations can provide information about human migrations. However, li...
The expression of Helicobacter pylori tfs plasticity zone cluster is regulated ...
Silva, Bruno; Nunes, Alexandra; Vale, Filipa; Rocha, Raquel; Gomes, João Paulo; Dias, Ricardo; Oleastro, MónicaBackground: Helicobacter pylori virulence is associated with different clinical outcomes. The existence of an intact dupA gene from tfs4b cluster has been suggested as a predictor for duodenal ulcer development. However, the role of tfs plasticity zone clusters in the development of ulcers remains unclear. We studied several H. pylori strains to characterize the gene arrangement of tfs3 and tfs4 clusters and th...
Proteome variability among Helicobacter pylori isolates clustered according to ...
Vitoriano, Inês; Vítor, Jorge; Oleastro, Mónica; Roxo-Rosa, Mónica; Vale, FilipaAims: To understand whether the variability found in the proteome of Helicobacter pylori relates to the genomic methylation, virulence and associated gastric disease. Methods and Results: We applied the Minimum-Common-Restriction- Modification (MCRM) algorithm to genomic methylation data of 30 Portuguese H. pylori strains, obtained by genome sensitivity to Type II restriction enzymes’ digestion. All the generat...
Screening of Prophage Sequences Among Helicobacter Pylori
Timóteo, Andreia; Breurec, S.; Oleastro, Mónica; Roxo-Rosa, Mónica; Vítor, Jorge; Lehours, Phillipe; Vale, FilipaUntil recently, Helicobacter pylori was considered a bacterium without prophages. The presence of an incomplete prophage sequence in strain B38 and a complete prophage sequence in strain B45 showed otherwise. Using a PCR strategy, based on degenerated primers designed after aligning bacteriophage integrase genes from H. pylori strains B38 and B45, and H. acinonychis prophage II, we found that integrase sequence...
The ulcerogenic profile of Helicobacter pylori paediatric strains associated wi...
Vitoriano, Inês; Saraiva-Pava, Kathy; Rocha-Gonçalves, António; Alves-Matos, Pedro; Vale, Filipa; Santos, Andrea; Lopes, Ana Isabel; Oleastro, MónicaHelicobacter pylori infection is the major cause of paediatric peptic ulcer disease (PUD). In children with no other aetiology for the disease, this rare event occurs shortly after infection, presuming a still poorly understood higher susceptibility of the patient and highlighting the virulence of the implicated strain. Recently, we showed that the enhanced virulence of a group of paediatric ulcerogenic-strains...