Despite the great commercial success, the Pacific white shrimp Litopenaeus vannamei farming has suffered great economic losses due to the impact of viral diseases such as White Spot Syndrome. Among the innovative ap-proaches to mitigate viral diseases, one of the most promising is the manipulation of the eukaryote process known as interfering RNA (RNAi). This natural mechanism can be induced to improve degradat...
The super-intensive BioFloc Technology (BFT) system has been highlighted as a promising eco-friendly alter-native to the traditional shrimp rearing systems. To gain insight into the impact of environmental rearingconditions on shrimp intestinal immunity, we assessed the expression profile of key immunological genes in themidgut ofLitopenaeus vannameishrimp reared in two contrasting culture systems: the indoor s...
Vibrio parahaemolyticus is a potentially pathogenic bacterium that occurs naturally in estuarine environments worldwide. This research aimed to investigate the occurrence of V. parahaemolyticus in estuarine environments and determine the virulence profile in an aquaculture environment by molecular techniques and conventional microbiological methods. Sampling was conducted in four estuaries in the State of Ceará...
Single nucleotide polymorphisms (SNPs) are the best genetic markers for associative studies of the immune system in invertebrates. In the marine shrimp Litopenaeus vannamei, SNPs linked to disease resistance have been reported for some genes, such as hemocyanin, anti-lipopolysaccharide factor, and heat-shock protein 70 (Hsp70). In the present study, polymorphisms in the Hsp70 gene were investigated among three ...
The diatomAsterionellopsis glacialissensu latoforms high-density patches in the surf zone of somesandy beaches worldwide and was until recentlyconsidered a cosmopolitan species. With the recentdescription of four cryptic species, the identity ofspecimens found in these accumulations remainsuncertain. In this study, diatom patches weresampled from two sandy beaches of the Braziliancoast: one tropical (Futuro Bea...
This study investigatedPerkinsusspp. infectingCrassostrea rhizophoraefrom the Jaguaribe River estuary,Ceará, Brazil. Fragments of gills and rectum of the oysters (n = 150) were incubated in Ray’s fluid thiogly-collate medium (RFTM). GenusPerkinsus-specific PerkITS85/750 PCR assays were performed and theiramplicons were sequenced by the Sanger method. The RFTM assays confirmedPerkinsusspp. Thesequencing of the a...
Endangered angelsharks and a protected dolphin species are illegally traded in Brazil. In this study, we determined prospective molecular markers for detecting these species in the trade of angelshark carcasses and ‘dolphin’ eyeball amulets. We compiled publicly available as well as new and unpublished cytochrome b (cyt b) DNA sequences for species involved in these trades. These sequences were digested in sili...
The objective of this work was to estimate the genetic variability level and distribution in Brazilian broodstocks of marine shrimp (Litopenaeus vannamei). Nine of the country's largest hatcheries were evaluated using codominant and highly polymorphic microsatellite markers. The results obtained from genotyping of ten microsatellite loci are indicative of genetic variability that is compatible with that found i...
The majority of fish species reared in captivity presents some form of reproductive dysfunction. In females may appear failures of final maturation of the ova, ovulation and the spawning; while in males occurs a decrease in the production of milt and in its quality. These dysfunctions appear due to the modification of the natural conditions of reproduction of the fish in captivity, originated from the lack of e...
The shrimp industry has grown significantly over the past 10 years in Brazil, especially the farmed production of the exotic Pacific white shrimp, Litopenaeus vannamei. In 2004, this industry was marked by a productivity crisis, which stirred interest towards genetic improvement of shrimp stocks. Shrimp breeders importation was banned in Brazil by a govern Normative Instruction in 1997, as a sanitary precaution...