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Challenges in imaging analyses of biomolecular condensates in cells infected wi...
Etibor, Temitope Akhigbe; O’Riain, Aidan; Alenquer, Marta; Diwo, Christian; Vale-Costa, Sílvia; Amorim, Maria JoãoBiomolecular condensates are crucial compartments within cells, relying on their material properties for function. They form and persist through weak, transient interactions, often undetectable by classical biochemical approaches. Hence, microscopy-based techniques have been the most reliable methods to detail the molecular mechanisms controlling their formation, material properties, and alterations, including ...
ATG9A regulates the dissociation of recycling endosomes from microtubules to fo...
Vale-Costa, Sílvia; Etibor, Temitope Akhigbe; Brás, Daniela; Sousa, Ana Laura; Ferreira, Mariana; Martins, Gabriel G.; Mello, Victor HugoAU It is:now Pleaseconfirmthatallheadinglevelsarerepresentedcorrectly established that many viruses that threaten public health : establish condensates via phase transitions to complete their lifecycles, and knowledge on such processes may offer new strategies for antiviral therapy. In the case of influenza A virus (IAV), liquid condensates known as viral inclusions, concentrate the 8 distinct viral ribonucleop...
ATG9A facilitates the biogenesis of influenza A virus liquid condensates near t...
Vale-Costa, Sílvia; Etibor, Temitope Akghibe; Brás, Daniela; Sousa, Ana Laura; Amorim, Maria JoãoMany viruses that threaten public health establish condensates via phase transitions to complete their lifecycles, and knowledge on such processes is key for the design of new antivirals. In the case of influenza A virus, liquid condensates known as viral inclusions are sites dedicated to the assembly of its 8-partite RNA genome. Liquid viral inclusions emerge near the endoplasmic reticulum (ER) exit sites, but...
Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reti...
Alenquer, Marta; Vale-Costa, Sílvia; Etibor, Temitope Akhigbe; Ferreira, Filipe; Sousa, Ana Laura; Amorim, Maria JoãoInfluenza A virus has an eight-partite RNA genome that during viral assembly forms a complex containing one copy of each RNA. Genome assembly is a selective process driven by RNA-RNA interactions and is hypothesized to lead to discrete punctate structures scattered through the cytosol. Here, we show that contrary to the accepted view, formation of these structures precedes RNA-RNA interactions among distinct vi...
Recycling Endosomes and Viral Infection
Vale-Costa, Sílvia; Amorim, MariaMany viruses exploit specific arms of the endomembrane system. The unique composition of each arm prompts the development of remarkably specific interactions between viruses and sub-organelles. This review focuses on the viral-host interactions occurring on the endocytic recycling compartment (ERC), and mediated by its regulatory Ras-related in brain (Rab) GTPase Rab11. This protein regulates trafficking from t...
Influenza A virus ribonucleoproteins modulate host recycling by competing with ...
Vale-Costa, Sílvia; Alenquer, Marta; Sousa, Ana Laura; Kellen, Bárbara; Ramalho, José; Tranfield, Erin M.; Amorim, Maria JoãoInfluenza A virus assembly is an unclear process, whereby individual virion components form an infectious particle. The segmented nature of the influenza A genome imposes a problem to assembly because it requires packaging of eight distinct RNA particles (vRNPs). It also allows genome mixing from distinct parental strains, events associated with influenza pandemic outbreaks. It is important to public health to ...
Clustering of Rab11 vesicles in influenza A virus infected cells creates hotspo...
Vale-Costa, Sílvia; Amorim, Maria JoãoInfluenza A virus is an important human pathogen causative of yearly epidemics and occasional pandemics. The ability to replicate within the host cell is a determinant of virulence, amplifying viral numbers for host-to-host transmission. This process requires multiple rounds of entering permissive cells, replication, and virion assembly at the plasma membrane, the site of viral budding and release. The assembly...
Studies in the mouse model identify strain variability as a major determinant o...
Marques, Filipe; Vale-Costa, Sílvia; Cruz, Tânia; Marques, Joana Moreira; Silva, Tânia; Neves, João Vilares; Cortes, Sofia; Fernandes, AnaVisceral leishmaniasis is a severe and potentially fatal disease caused by protozoa of the genus Leishmania, transmitted by phlebotomine sandflies. In Europe and the Mediterranean region, L. infantum is the commonest agent of visceral leishmaniasis, causing a wide spectrum of clinical manifestations, including asymptomatic carriage, cutaneous lesions and severe visceral disease. Visceral leishmaniasis is more f...
Iron Overload Favors the Elimination of Leishmania infantum from Mouse Tissues ...
Vale-Costa, Sílvia; Gomes Pereira, Sandra Isabel; Teixeira, Carlos Miguel; Rosa, Gustavo; Rodrigues, Pedro Nuno; Tomás, Ana; Appelberg, RuiIron plays a central role in host-parasite interactions, since both intervenients need iron for survival and growth, but are sensitive to iron-mediated toxicity. The host’s iron overload is often associated with susceptibility to infection. However, it has been previously reported that iron overload prevented the growth of Leishmania major, an agent of cutaneous leishmaniasis, in BALB/c mice. In order to furthe...