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Yeast extract elicitation enhances growth and metabolite production in Limonium...

Lescano, Leonardo; Cziáky, Zoltán; Custódio, Luísa; Rodrigues, Maria João

Limonium algarvense Erben, a medicinal halophyte, holds significant pharmacological promise due to its rich bioactive compound repertoire. This study aimed to establish robust callus cultures as a sustainable, in vitro model for studying the plant's metabolic responses, particularly focusing on synthesising and accumulating primary and secondary metabolites under various elicitation treatments. Callus cultures ...


Physicochemical, nutritional, and antioxidant properties of yogurt fortified wi...

Bratkič, Kim; Rodrigues, Maria João; Castañeda-Loaiza, Viana; Guerreiro Pereira, Catarina Alexandra; Ratao, Isabel; Quintas, Célia

Biotechnological valorization of invasive species supports sustainable management by transforming ecological threats into valuable resources. While Carpobrotus edulis fruits are rich in bioactive compounds, their use as functional ingredients remains unexplored. This study assessed the feasibility of incorporating C. edulis fruit extracts into yogurt to enhance its functional properties. To achieve this, water,...


Antioxidant activity, enzyme inhibition, photoprotection, cytotoxicity, and phy...

Lescano, Leonardo; Cziáky, Zoltán; Kurt-Celep, İnci; Zengin, Gökhan; Fernandes, Eliana; Trentin, Riccardo; Guerreiro Pereira, Catarina Alexandra

Despite sea lavender being a medicinal species, research on its seeds' biological properties and chemical composition is unexplored. Thus, this study evaluated the effect of different extraction solvents on the biological activities and chemical profile of greenhouse-cultivated sea lavender seeds, aiming at their potential use as a dermo-cosmetic ingredient. Therefore, ethyl acetate, acetone, ethanol, and water...


Characterising the metabolomic diversity and biological potentials of extracts ...

Ahmed, Shakeel; Zengin, Gokhan; Selvi, Selami; Ak, Gunes; Cziáky, Zoltán; Jekő, József; Rodrigues, Maria João; Custódio, Luísa; Venanzoni, Roberto

This study investigates the biochemical composition and biological properties of different parts (leaves, roots, and twigs) of two Cistus species (Cistus monspeliasis and Cistus parviflorus). The extracts were analysed using UHPLC-MS/MS to determine their chemical profiling. A range of antioxidant assays were performed to evaluate the extract’s antioxidant capabilities. The enzyme inhibition studies focused on ...


Establishment and elicitation of liquid adventitious root cultures of Inula cri...

Custódio, Luísa; Cziáky, Zoltán; Castañeda-Loaiza, Viana; Rodrigues, Maria Joao

This study established a liquid adventitious root culture for Inula crithmoides L. focusing on producing extracts rich in hepatoprotective caffeoylquinic acids through elicitation. Adventitious roots were induced from in vitro-grown plant leaves cultured on MS medium supplemented with 1 mg/L IBA. Growth and secondary metabolites accumulation (phenolics, flavonoids, hydroxycinnamic acids) were monitored weekly t...


Harnessing the bioactive potential of Limonium spathulatum (Desf.) kuntze: insi...

Youssef, Seria; Custódio, Luísa; Rodrigues, Maria João; Pereira, Catarina G.; Calhelha, Ricardo C.; Jekő, József; Cziáky, Zoltán; Ben Hamed, Karim

This study assessed the halophyte species Limonium spathulatum (Desf.) as a possible source of natural ingredients with the capacity to inhibit enzymes related to relevant human health disorders and food browning. Extracts using food-grade solvents such as water and ethanol were prepared by maceration from dried L. spathulatum leaves. They were evaluated for in vitro inhibition activity of enzymes such as acety...

Data: 2023   |   Origem: Biblioteca Digital do IPB

Harnessing the bioactive potential of Limonium spathulatum (Desf.) Kuntze: insi...

Youssef, Seria; Custódio, Luísa; Rodrigues, Maria João; Pereira, Catarina; Calhelha, Ricardo C.; Jekő, József; Cziáky, Zoltán; Ben Hamed, Karim

This study assessed the halophyte species <i>Limonium spathulatum</i> (Desf.) as a possible source of natural ingredients with the capacity to inhibit enzymes related to relevant human health disorders and food browning. Extracts using food-grade solvents such as water and ethanol were prepared by maceration from dried <i>L. spathulatum</i> leaves. They were evaluated for in vitro inhibition activity of enzymes...


Nutritional anti-nutritional chemical composition and antioxidant activities of...

Youssef, Seria; Custódio, Luísa; Rodrigues, Maria João; Pereira, Catarina G.; Calhelha, Ricardo C.; Pinela, José; Barros, Lillian; Jekő, József

This work explored the nutritional and antioxidant properties of the leaves of the halophytic species Limonium spathulatum (Desf.) Kuntze from Tunisian sea cliffs. Furthermore, the analysis of the total phenolics and flavonoids contents and their individual compounds using high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS) were also studied. L. spathul...

Data: 2022   |   Origem: Biblioteca Digital do IPB

Nutritional and phyto-therapeutic value of the Halophyte Cladium mariscus L. (P...

Rodrigues, Maria João; L, Custódio; Mecha, Débora; Zengin, Gokhan; Cziáky, Zoltán; Sotkó, Gyula; Pereira, Catarina Guerreiro

This work searched for the phyto-therapeutic potential and nutritional value of seeds from the halophyte Cladium mariscus L. (Pohl.), aiming at its use as a source of bioactive ingredients for the food industry. Hence, the nutritional profile, including minerals, of seeds biomass was determined; food-grade samples were prepared, and their phytochemical fingerprinting assessed. Extracts were evaluated for in vit...


The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidan...

Rodrigues, Maria João; Jekő, József; Cziáky, Zoltán; Pereira, Catarina Guerreiro; L, Custódio

This work explored the medicinal halophyte Frankenia laevis L. (sea heath) as a potential source of bioactive natural products. In this sense, methanol and dichloromethane extracts were prepared from aerial organs containing flowers, leaves and stems, and were profiled for their chemical composition using high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/...


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