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Inclusion of pineapple by-products as natural antioxidant sources in diets for ...

Pereira, Ricardo; Basto, Ana; Pintado, Manuela; Valente, Luisa M. P.; Velasco, Cristina

This study investigates the effects of pineapple by-products on feed preservation during storage at two different temperatures (25 °C and 4 °C) and on European sea bass (Dicentrarchus labrax) stress resistance. Four isoproteic, isolipidic, and isoenergetic diets were manufactured: CTRL—negative control, commercial diet without added antioxidants; VITE—positive control, CTRL diet with 100 mg kg−1 of vitamin E; a...


Circular animal protein hydrolysates: a comparative approach of functional prop...

Monteiro, Marta; Rodrigues-dos-Santos, Luciano; Filipa-Silva, Andreia; Marques, Diana A.; Pintado, Manuela; Almeida, André; Valente, Luisa M. P.

The growing demand for nutraceuticals has driven interest in upcycling low-value proteins from processed animal by-products and insect larvae into functional protein hydrolysates. This study evaluated five such hydrolysates in comparison to a high-value commercial reference (CPSP90), assessing the proximate composition, amino acid profile, molecular weight distribution, antioxidant activity, and bacterial growt...


Locally-sourced animal protein hydrolysates in high-plant-protein diets can pro...

Rodrigues-dos-Santos, Luciano; Basto, Ana; Monteiro, Marta; António, Carla; Rodrigues, Ana M.; Sá, Tiago; Velasco, Cristina; Martins, Rui; Rosa, Ana

Upcycling meat and fish by-products into bioactive protein hydrolysates promotes zero-waste practices within the circular bioeconomy and provides locally-sourced alternatives to replace fishmeal (FM) in aquafeeds. In this study, novel hydrolysates were developed from blue shark skin, fish by-products, and swine processed animal protein, aiming to reduce the high-quality FM inclusion in European seabass (Dicentr...


Unravelling the effects of extrusion and drying temperatures on the radical sca...

Pereira, Ricardo; Velasco, Cristina; Gómez-Garcia, Ricardo; Dias, Jorge; Pintado, Manuela; Valente, Luisa M. P.

Antioxidant supplementation of aquafeeds with natural sources is a current research trend within the context of a circular economy. However, natural antioxidants are highly vulnerable to thermal conditions during feed manufacturing, particularly during extrusion and drying. This study examines the impact of extrusion and drying temperatures on the antioxidant properties of aquafeeds supplemented with natural an...


Incorporating sardine cooking water aromas into plant-based diets for european ...

Resende, Daniela; Velasco, Cristina; Pereira, Maria J.; Sá, Tiago; Rocha, Célia; Cunha, Luís M.; Lima, Rui C.; Brazinha, Carla; Pintado, Manuela

Sardine cooking wastewaters are by-products of the canning industry with great potential for valorisation. We have hypothesized that they can be a source of aromas to enhance appetite when added to plant-based diets. The poor palatability of such diets often described in carnivorous species poses a recurring problem in fish farming, with harsh consequences on fish growth performance and flesh quality. Aromas fr...


Production of aroma-rich extracts from sardine cooking wastewaters: exploring t...

Resende, Daniela; Pereira, Maria J.; Sá, Tiago; Brazinha, Carla; Pintado, Manuela; Valente, Luisa M. P.; Velasco, Cristina

Managing the canning industry’s nutrient-rich and odorous liquid waste is a hurdle. Concurrently, the growing use of vegetable ingredients in aquafeeds diminishes palatability and feed consumption in carnivorous fish. Thus, we hypothesized that aromas could be extracted from cooking wastewaters at canning factories and added to plant-based diets to stimulate intake in European seabass. Sardine cooking wastewate...


Dietary inclusion of blood hydrolysates affects muscle growth in European seaba...

Velasco, Cristina; Resende, Daniela; Oliveira, Beatriz; Canada, Paula; Pereira, Miguel; Pereira, Carlos; Pintado, Manuela; Valente, Luisa M. P.

Dietary supplementation with hydrolysates has been suggested to influence muscle protein synthesis and fish growth. This study assessed the impact of including 3% swine blood hydrolysates (BH) in a plant-based diet on muscle cellularity and the expression of molecular markers related to muscle fibre proliferation and hypertrophic growth of European seabass. Three BH fractions were obtained by two different proc...


Biofortified Diets Containing Algae and Selenised Yeast: Effects on Growth Perf...

Ferreira, Mariana; Ribeiro, Pedro C.; Ribeiro, Laura; Barata, Marisa; Domingues, Valentina F.; Sousa, Sara; Soares, Cristina; Marques, Alexandra

Efforts have been made to find natural, highly nutritious alternatives to replace fish meal (FM) and fish oil (FO), which can simultaneously promote fish health and improve the nutritional quality of filets for human consumption. This study evaluated the impact of biofortified diets containing microalgae (as replacement for FM and FO), macroalgae (as natural source of iodine) and selenised yeast (organic source...


Innovative swine blood hydrolysates as promising ingredients for European seaba...

Resende, Daniela; Costas, Benjamin; Sá, Tiago; Golfetto, Umberto; Machado, Marina; Pereira, Miguel; Pereira, Carlos; Marques, Bianca

Hydrolysates from high-protein coproducts are rich in bioactive peptides (small amino acid chains with properties beyond their nutritional value). When included in aquafeeds, they may promote fish robustness while increasing animal blood value and minimizing waste, contributing to a circular economy. This work assessed how innovatively produced blood hydrolysates (BH) included in plant-based diets for European ...


The use of defatted Tenebrio molitor larvae meal as a main protein source is su...

Basto, Ana; Calduch-Giner, Josep; Oliveira, Beatriz; Petit, Lisa; Sá, Tiago; Maia, Margarida R. G.; Fonseca, Susana C.; Matos, Elisabete

Objective: This study aims to determine the maximal inclusion level of defatted (d-) Tenebrio molitor larvae meal (TM) able to replace dietary fishmeal (FM) without compromising growth performance, general metabolism, and flesh quality traits in European sea bass, and to evaluate the major underlying physiological mechanisms. Materials and Methods: Fish (55 ± 2 g) were fed with diets containing increasing level...


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