8 documents found, page 1 of 1
Optimal conditions for biomass and recombinant glycerol kinase production using...
Aizemberg, Raquel [UNESP]; Terrazas, Werner D.M. [UNESP]; Ferreira-Dias, Suzana; Valentini, Sandro R. [UNESP]; Gattás, Edwil A.L. [UNESP]Made available in DSpace on 2022-04-28T18:57:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-07-01; The extracellular glycerol kinase gene from Saccharomyces cerevisiae (GUT1) was cloned into the expression vector pPICZα A and integrated into the genome of the methylotrophic yeast Pichia pastoris X-33. The presence of the GUT1 insert was confirmed by PCR analysis. Four clones were selected and the fun...
The methionine 549 and leucine 552 residues of friedelin synthase from maytenus...
Mazzeu, Bruna F. [UNESP]; Souza-Moreira, Tatiana M. [UNESP]; Oliveira, Andrew A.; Remlinger, Melissa [UNESP]; Felippe, Lidiane G. [UNESP]Made available in DSpace on 2022-04-29T08:46:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-02; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Friedelin, a pentacyclic triterpene found in the leaves of the Celastraceae species, demon-strates numerous biological activities and is a precursor of quinonemethide trite...
Biosynthetic insights into p-Hydroxybenzoic acid-Derived benzopyrans in piper g...
Batista, Andrea N. L. [UNESP]; Batista, João M.; Souza‑Moreira, Tatiana M. [UNESP]; Valentini, Sandro R. [UNESP]; Kato, Massuo J.Made available in DSpace on 2018-12-11T17:36:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-05-01. Added 1 bitstream(s) on 2019-10-09T18:33:08Z : No. of bitstreams: 1 S0103-50532018000501105.pdf: 2907602 bytes, checksum: ef8123c16f0caba93af7dd13a547df64 (MD5); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Département Caractérisation et Élaboration des Produits Issus de l’Agriculture,...
Friedelin in maytenus ilicifolia is produced by friedelin synthase isoforms
Alves, Thaís B. [UNESP]; Souza-Moreira, Tatiana M. [UNESP]; Valentini, Sandro R. [UNESP]; Zanelli, Cleslei F. [UNESP]; Furlan, Maysa [UNESP]Made available in DSpace on 2018-12-11T17:36:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01; Triterpenes are interesting compounds because they play an important role in cell homeostasis and a wide variety exhibiting defense functions is produced by plant secondary metabolism. Those same plant secondary metabolites also exhibit biological properties with promising therapeutic potential as anti-...
Screening of 2A peptides for polycistronic gene expression in yeast
Souza-Moreira, Tatiana M. [UNESP]; Navarrete, Clara; Chen, Xin; Zanelli, Cleslei F. [UNESP]; Valentini, Sandro R. [UNESP]; Furlan, Maysa [UNESP]Made available in DSpace on 2018-12-11T17:21:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-08-01; A complexity of pathway expression in yeast compared to prokaryotes is the need for separate promoters and terminators for each gene expressed. Single transcript expression and separated protein production is possible via the use of 2A viral peptides, but detailed characterization to assess their suitab...
Friedelin Synthase from Maytenus ilicifolia: Leucine 482 Plays an Essential Rol...
Souza-Moreira, Tatiana M. [UNESP]; Alves, Thaís B. [UNESP]; Pinheiro, Karina A. [UNESP]; Felippe, Lidiane G. [UNESP]; De Lima, Gustavo M. A.Made available in DSpace on 2018-12-11T17:23:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-11-22; Among the biologically active triterpenes, friedelin has the most-rearranged structure produced by the oxidosqualene cyclases and is the only one containing a cetonic group. In this study, we cloned and functionally characterized friedelin synthase and one cycloartenol synthase from Maytenus ilicifolia ...
Evidence for a negative cooperativity between eIF5A and eEF2 on binding to the ...
Rossi, Danuza [UNESP]; Barbosa, Natalia M. [UNESP]; Galvão, Fabio C. [UNESP]; Boldrin, Paulo E. G. [UNESP]; Hershey, John W. B.Made available in DSpace on 2018-12-11T17:04:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-04-01; National Institute of General Medical Sciences; eIF5A is the only protein known to contain the essential and unique amino acid residue hypusine. eIF5A functions in both translation initiation due to its stimulation of methionyl-puromycin synthesis and translation elongation, being highly required for pe...
Mapping surface residues of eIF5A that are important for binding to the ribosom...
Barbosa, Natália M. [UNESP]; Boldrin, Paulo E. G. [UNESP]; Rossi, Danuza [UNESP]; Yamamoto, Priscila A. [UNESP]; Watanabe, Tatiana F. [UNESP]Made available in DSpace on 2018-12-11T17:04:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-10-01; The translation elongation factor eIF5A is conserved through evolution and is necessary to rescue the ribosome during translation elongation of polyproline-containing proteins. Although the site of eIF5A binding to the ribosome is known, no systematic analysis has been performed so far to determine the ...