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Performance evaluation of an indirect immunofluorescence kit for the serologica...

Arai,Karina Emy; Bo,Carolina Rodrigues Dal; Silva,Ana Paula Marques Aguirra da; Rodrigues,Silvia Sanches; Mangueira,Cristóvão Luis Pitangueira

ABSTRACT Objective: To evaluate the performance of indirect immunofluorescence for serological diagnosis of dengue virus in a population with high prevalence of arboviruses. Methods: Two-hundred serum samples from patients with clinical suspicion of dengue fever were tested by immunoenzymatic and indirect immunofluorescence assay BIOCHIP® mosaic. Specificity, sensitivity and Kappa coefficient were calculated. D...

Date: 2020   |   Origin: Oasisbr

Comparison between enzyme-linked immunosorbent assay and indirect immunofluores...

Menezes,Julia Miranda; Rossener,Raissa; Silva,Ana Paula Marques Aguirra da; Rodrigues,Silvia Sanches; Mangueira,Cristóvão Luis Pitangueira

ABSTRACT Objective To evaluate the performance of enzyme-linked immunosorbent assay and indirect immunofluorescence methods for the detection of antineutrophil cytoplasmic antibodies in a routine clinical laboratory setting. Methods A total of 227 samples were tested by indirect immunofluorescence and enzyme-linked immunosorbent assay with antigen specificity for antiproteinase 3 and antimyeloperoxidase. The pr...

Date: 2020   |   Origin: Oasisbr

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