6 documents found, page 1 of 1

State and specific growth estimation in heterologous protein production by Pich...

The productivity of an industrial fermentation process involving a filamentous microbe is heavily dependent on the morphological form adopted by the organism. The development of systems capable of rapidly and accurately characterizing morphology within a given process represents a significant challenge, as the complex phenotypes that are manifested are not easily quantified. Conventional parameters employed in ...

Optimized production of MLM Triacylglycerols catalyzed by immobilized heterolog...

Response surface methodology was used to model and optimize the acidolysis of virgin olive oil with caprylic (C8:0) or capric (C10:0) acids, aimed at the production of low caloric triacylglycerols (TAG) of MLM type, in solvent free media, catalyzed by the heterologous Rhizopus oryzae lipase (r-ROL) immobilized in Eupergit(A (R)) C. This lipase was produced in the methylotrophic yeast Pichia pastoris Mut(s) phen...

Batch operational stability of immobilized heterologous Rhizopus oryzae lipase ...

Structured triacylglycerols containing medium-chain fatty acids (M) at positions sn-1,3 and long-chain fatty acids (L) at the sn-2 position (MLM type), were obtained by acidolysis of virgin olive oil with caprylic or capric acid, in solvent-free media, at 40 degrees C, catalyzed by a heterologous Rhizopus oryzae lipase (r-ROL) immobilized in Eupergir (R) C or in Lewatit VP OC 1600. The biocatalyst immobilized i...

Production of MLM-Type structured lipids catalyzed by immobilized heterologous ...

This work aims to produce triacylglycerols (TAG) containing a medium-chain fatty acid (M) at positions sn-1,3 and a long-chain fatty acid (L) at sn-2 position, i.e. TAG of MLM type, by acidolysis of virgin olive oil with caprylic (C8:0) or capric (C10:0) acids, catalyzed by 1,3-selective Rhizopus oryzae heterologous lipase (rROL) immobilized in Eupergit (R) C and modified sepiolite. This lipase was produced by ...

Observer based estimation in heterologous protein production by Pichia pastoris

The methylotrophic yeast Pichia pastoris has been widely reported as a suitable expression system for both basic research and industrial use. In order to achieve high productivity, constant product quality, as well as to allow optimization and control of biotechnological processes, real-time monitoring of the key fermentation variables (biomass, substrate and products) is of major academic and industrial releva...

Calibration of a simplified model describing an urban waste water treatment pil...