Objective Key BV-associated bacteria that may be important in incident BV (iBV) pathogenesis are Gardnerella species and Prevotella bivia. We quantified the amount of these bacterial species in clue cell biofilms in longitudinal vaginal specimens from iBV cases and controls using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH). Study Design Women with optimal vaginal microbiota self-collected...
[Excerpt] OBJECTIVES: ln an ongoing community-based BV pathogenesis study, we evaluated time to incident BV (iBV) as well as select characteristics of women with this infection. METHODS: Non-pregnant women ages 18-45 with normal vaginal microbiota (no Amsel criteria, normal Nugent score), no antibiotic use in the past 14 days, no concurrent STis, and a current male sexual partner were followed for 9 weeks. Part...