10 documents found, page 1 of 1
In vitro interactions within a biofilm containing three species found in bacter...
Rosca, Aliona; Castro, Joana Isabel Reis; Sousa, Lúcia; França, Ângela Maria Oliveira Sousa; Vaneechoutte, Mario; Cerca, NunoBacterial vaginosis (BV), the most common cause of vaginal discharge, is characterized by the presence of a polymicrobial biofilm on the vaginal epithelium, formed primarily by Gardnerella spp., but also other anaerobic species. Interactions between bacteria in multi-species biofilms are likely to contribute to increased virulence and to enhanced antimicrobial tolerance observed in vivo. However, functional stu...
Gardnerella vaginalis dominates multi-species biofilms in both pre-conditioned ...
Rosca, Aliona; Castro, Joana Isabel Reis; França, Ângela Maria Oliveira Sousa; Vaneechoutte, Mario; Cerca, NunoThe online version contains supplementary material available at: https://doi.org/10.1007/s00248-021-0; Bacterial vaginosis (BV) is one of the most common bacterial vaginal infections worldwide. Despite its high prevalence, BV etiology is still unknown. Nevertheless, a hallmark of BV is the presence of a highly structured polymicrobial biofilm on the vaginal epithelium, formed primarily by Gardnerella spp. and o...
In vitro interactions during bacterial vaginosis development demonstrate that m...
Rosca, Aliona; Sousa, Lúcia Filipa Guimarães Vieira; Castro, Joana Isabel Reis; França, Ângela Maria Oliveira Sousa; Vaneechoutte, Mario; Cerca, NunoBackground. Bacterial vaginosis (BV) is the worldwide leading vaginal bacterial infection commonly identified between menarche and menopause in women of all ethnicities. It is associated with serious health problems relating to both fertility and pregnancy. The hallmark of BV is the presence of a polymicrobial biofilm on the vaginal epithelium, formed mainly by Gardnerella spp., followed by Fannyhessea vaginae,...
Gardnerella vaginalis enhances Atopobium vaginae viability in an in vitro Model
Castro, Joana Isabel Reis; Rosca, Aliona; Cools, Piet; Vaneechoutte, Mario; Cerca, NunoBacterial vaginosis (BV) is the most common vaginal infection among women of reproductive age. A hallmark of BV is the presence of a highly structured polymicrobial biofilm on the vaginal epithelium, presumably initiated by facultative anaerobes of the genus Gardnerella, which then becomes a scaffold for other species to adhere to. One of the species often found incorporated in Gardnerella mediated biofilms is ...
Gardnerella spp. pre-conditioned vs competitive multi-species biofilm growth an...
Rosca, A.; Vaneechoutte, Mario; Cerca, NunoBackground: Bacterial vaginosis (BV) is one of the most common bacterial vaginal disorders among women of reproductive age. The hallmark of BV is the presence of a multi-species biofilm, formed primarily by Gardnerella spp., in a minor part by Atopobium vaginae, and also other anaerobic species. While a few studies have demonstrated that some BV-related species establish synergistic interactions with Gardnerell...
Polymicrobial infections and biofilms in women's health Gahro Expert Group Meet...
Cerca, Nuno; Vaneechoutte, Mario; Guschin, Alexander; Swidsinski, Alexanderinfo:eu-repo/semantics/publishedVersion
Bacterial biofilms in the vagina
Hardy, Liselotte; Cerca, Nuno; Jespers, Vicky; Vaneechoutte, Mario; Crucitti, TaniaA bacterial biofilm is a structured community of bacteria in a self-produced extracellular matrix, adherent to an inert surface or biological tissue. The involvement of biofilm in a bacterial infection implies that the infection is difficult to treat and that the patient will probably experience relapses of the condition. In bacterial vaginosis (BV), the lactobacilli concentration decreases, while the bacterial...
Fluorescence in situ hybridization method using peptide nucleic acid probes for...
Machado, António; Almeida, Carina; Salgueiro, D. A. L.; Henriques, Ana Filipa Frutuoso Mendes; Vaneechoutte, Mario; Haesebrouck, Freddy; Vieira, M. J.Background: Bacterial vaginosis (BV) is a common vaginal infection occurring in women of reproductive age. It is widely accepted that the microbial switch from normal microflora to BV is characterized by a decrease in vaginal colonization by Lactobacillus species together with an increase of Gardnerella vaginalis and other anaerobes. Our goal was to develop and optimize a novel Peptide Nucleic Acid (PNA) Fluore...
Detection of Lactobacillus and Gardnerella species in vaginal samples by PNA-FISH
Machado, António; Salgueiro, Débora; Almeida, Carina; Henriques, Ana Filipa Frutuoso Mendes; Haesebrouck, Freddy; Vaneechoutte, Mario; Rodrigues, L. R.Bacterial vaginosis (BV) is a common vaginal infection of women in reproductive age. This infection is initially asymptomatic and late diagnosis can increase the health costs and hamper treatment. Therefore, an easy and quick method to accurately detect the transition between normal microflora to the predecessors of infection is of upmost importance. Despite the fact that BV etiology remains unknown, it is comm...
The phage therapy paradigm: prêt-à-porter or sur-mesure?
Pirnay, Jean-Paul; De Vos, Daniel; Verbeken, Gilbert; Merabishvili, Maia; Chanishvili, Nina; Vaneechoutte, Mario; Zizi, Martin; Laire, GeertThe present opinion is the result of discussions on the future of phage therapy (personalized or large-scale uniform therapy?) during the first International Congress on Viruses of Microbes, held at the Institut Pasteur in Paris on June 21–25, 2010.