Fungi are important biological elements in the Pine wilt disease (PWD) complex. In the late stages of the disease, the pinewood nematode (PWN) Bursaphelenchus xylophilus feeds on the fungal flora available in the pine tree for survival and multiplication. Previous studies have confirmed a close relation between the PWN and blue-stain fungi (Ophiostomatales), which are necrotrophic pathogens associated with bark...
O nemátode-das-lesões-radiculares (NLR), Pratylenchus penetrans, é um endoparasita migratório que afeta culturas agrícolas economicamente importantes, como a batata, estando atualmente presente na lista de pragas reguladas não sujeitas a quarentena A2 da União Europeia (UE 2016/2031). Em alguns países europeus, como Portugal, esta espécie foi detetada em campos de produção de batata estando relacionada com o se...
Bacteria harboring conjugative plasmids have the potential for spreading antibiotic resistance through horizontal gene transfer. It is described that the selection and dissemination of antibiotic resistance is enhanced by stressors, like metals or antibiotics, which can occur as environmental contaminants. This study aimed at unveiling the composition of the conjugative plasmidome of a hospital effluent multidr...
Members of the genus Aeromonas are recognized carriers of antibiotic resistance in aquatic environments. However, their importance on the spread of resistance from hospital effluents to the environment is poorly understood. Quinolone resistant Aeromonas spp. (n = 112) isolated from hospital effluent (HE) and from raw (RWW) and treated wastewater (TWW) of the receiving urban wastewater treatment plant (UWTP) wer...
This study is aimed to assess if hospital effluents represent an important supplier of multidrug-resistant (MDR) Gram-negative bacteria that, being discharged in the municipal collector, may be disseminated in the environment and bypassed in water quality control systems. From a set of 101 non-Escherichia coli Gram-negative bacteria with reduced susceptibility to quinolones, was selected a group of isolates com...
Escherichia coli with reduced susceptibility to quinolones isolated from different environmental sources (urban wastewater treatment plants, n = 61; hospital effluent, n = 10; urban streams, n = 9; gulls, n = 18; birds of prey, n = 17) and from hospitalised patients (n = 28) were compared based on multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). The habitats with the most diversifi...
Escherichia coli with reduced susceptibility to ciprofloxacin, isolated from urban streams, wastewater treatment plants and hospital effluent between 2004 and 2012, were compared based on multilocus sequence typing (MLST), quinolone and beta-lactam resistance determinants and plasmid replicon type. Isolates from the different types of water and isolation dates clustered together, suggesting the persistence and ...
Escherichia coli with reduced susceptibility to quinolones isolated from different environmental sources (urban wastewater treatment plants, n = 61; hospital effluent, n = 10; urban streams, n = 9; gulls, n = 18; birds of prey, n = 17) and from hospitalised patients (n = 28) were compared based on multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). The habitats with the most diversifi...
The relationship between antimicrobial residues, antibiotic resistance prevalence and bacterial community composition in hospital effluent and in the receiving wastewater treatment plant was studied. Samples from hospital effluent, raw inflow and final effluent of the receiving wastewater treatment plant were characterized for amoxicillin and ciprofloxacin resistance prevalence, content of heavy metals and anti...
The influence of geographic distribution and type of habitat on the molecular epidemiology of ciprofloxacin resistant Escherichia coli was investigated. Ciprofloxacin resistant E. coli from wastewater, urban water with faecal contamination and faeces of gulls, pigeons and birds of prey, from Portugal, Spain and Sweden were compared based on multilocus sequence typing (MLST) and quinolone resistance genetic dete...