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The Ocean’s pharmacy: Health discoveries in marine algae

Silva, Mélanie; Avni, Dorit; Varela, João; Barreira, Luísa

Non-communicable diseases (NCDs) represent a global health challenge, constituting a major cause of mortality and disease burden in the 21st century. Addressing the prevention and management of NCDs is crucial for improving global public health, emphasizing the need for comprehensive strategies, early interventions, and innovative therapeutic approaches to mitigate their far-reaching consequences. Marine organi...


Amino acids profile of 56 species of microalgae reveals that free amino acids a...

León-Vaz, Antonio; Giráldez, Inmaculada; Moreno-Garrido, Ignacio; Varela, João; Vigara, Javier; León, Rosa; Cañavate, José Pedro

Microalgae represent a phototrophic resource with a high protein content, whose nutritional value is very high as a result of its amino acid profile. Although the total amino acid profile has been repeatedly described to change little among phylogenetic groups of microalgae, some variability has occasionally been reported. Part of this uncertainty is associated with the low phylogenetic diversity encompassed in...


Pilot scale production of Crocosphaera chwakensis CCY0110 and evaluation of its...

Matinha-Cardoso, Jorge; Santos, Tamara; Pereira, Hugo; Varela, João; Tamagnini, Paula; Mota, Rita

In recent years, the large-scale cultivation and commercialization of microalgae/cyanobacteria biomasses have become a worldwide trend, mainly directed to the animal and human nutrition markets due to their outstanding nutritional value. However, only a very limited number are currently classified as food ingredients by Food Safety regulators worldwide. Crocosphaera chwakensis CCY0110 is a marine unicellular cy...


Heterotrophic and photoautotrophic media optimization using response surface me...

Correia, Nádia; Pereira, Hugo; Schulze, Peter S.C.; Costa, Monya; Santo, Gonçalo E.; Guerra, Inês; Trovão dos Santos, Mafalda; Barros, Ana

The nutritional requirements of novel microalgal strains are key for their effective cultivation and metabolite content. Therefore, the optimization of heterotrophic and photoautotrophic culture media is crucial for novel Chlorococcum amblystomatis growth. Heterotrophic and photoautotrophic biomass samples were characterized to identify the differences between their heterotrophic and photoautotrophic biomass co...


Dunaliella viridis TAV01: A halotolerant, Protein-Rich Microalga from the Algar...

Bombo, Gabriel; Cristofoli, N. L.; Santos, Tamara; Schüler, Lisa M.; Maia, Inês Beatriz; Pereira, Hugo; Barreira, Luísa; Varela, João

Tolerance to harsh environmental conditions, high growth rates and an amino acid profile adequate for human consumption are beneficial features observed in Dunaliella viridis TAV01, a novel strain isolated from a salt pond in the Algarve, Portugal. TAV01 was identified down to the species level by maximum likelihood and Bayesian phylogenetic analyses of the ribosomal internal transcribed spacers one and two reg...


Structural characterization of exopolysaccharides obtained from Porphyridium cr...

Cristofoli, Nathana L.; Ribeiro Lima, Alexandre; Rosa Da Costa, Ana; Evtyugin, Dmitry; Silva, Carlos; Varela, João; Vieira, Margarida

Microalgae produce and secrete large quantities of polysaccharides into the culture medium that is discarded when biomass is separated. The main objective of this study was to add value to the exhausted culture medium (ECM) of Porphyridium cruentum through the recovery of extracellular polysaccharides (EPS) by different extracting and purifying methods for the future biotechnological applications. The ECM was s...


Two-stage lipid induction in the microalga tetraselmis striata CTP4 upon exposu...

Monteiro, Ivo; Schüler, Lisa M.; Santos, Eunice; Pereira, Hugo; Schulze, Peter S.C.; Florindo, Claudia; Varela, João; Barreira, Luísa

Tetraselmis striata CTP4 is a euryhaline, robust, fast-growing microalga suitable for wastewater treatment and industrial production. Lipid production was induced through a two-stage cultivation strategy: a 1st stage under standard growth-promoting conditions (100 mu mol photons m- 2 s- 1, salinity 36 ppt and 20 degrees C) to achieve high biomass concentration and a 2nd stage of 6 days for lipid induction by th...


Pharmaceuticals removal from wastewater with microalgae: a pilot study

Pereira, André; Morais, Etiele Greque; Silva, Liliana; Pena, Angelina; Freitas, Andreia; Teixeira, Margarida Ribau; Varela, João; Barreira, Luísa

Urban wastewaters contain pharmaceuticals that are not appropriately removed in conventional wastewater treatments, limiting treated water reuse. Microalgae have been shown to remove pharmaceuticals from urban wastewater in laboratory trials, but few studies have been conducted under natural conditions. In this work, pharmaceutical removal was assessed in a pilot-scale microalgal tertiary wastewater treatment i...


Structural characterization of exopolysaccharides obtained from Porphyridium cr...

Cristofoli, Nathana L.; Lima, Alexandre R.; Costa, Ana M. Rosa da; Evtyugin, Dmitry; Silva, Carlos; Varela, João; Vieira, Margarida C.

Microalgae produce and secrete large quantities of polysaccharides into the culture medium that is discarded when biomass is separated. The main objective of this study was to add value to the exhausted culture medium (ECM) of Porphyridium cruentum through the recovery of extracellular polysaccharides (EPS) by different extracting and purifying methods for the future biotechnological applications. The ECM was s...


The osteogenic and mineralogenic potential of the microalgae Skeletonema costat...

Carletti, Alessio; Rosa, Joana; Pes, Katia; Borges, Inês; Santos, Tamara; Barreira, Luísa; Varela, João; Pereira, Hugo; Cancela, M. Leonor

Skeletal disorders are problematic aspects for the aquaculture industry as skeletal deformities, which affect most species of farmed fish, increase production costs and affect fish welfare. Following recent findings that show the presence of osteoactive compounds in marine organisms, we evaluated the osteogenic and mineralogenic potential of commercially available microalgae strains Skeletonema costatum and Tet...


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