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Bioremediation of drainwater from soilless cultivation by Tetradesmus obliquus ...

Maia, Inês Beatriz; Pinto, Bruno; Carneiro, Mariana; Konucu, Merve; Schulze, Peter S.C.; Santos, Tamara; Rodrigues, Alexandre M.C.

To meet the demands of the growing population, agricultural practices have been increasing and putting a strain on land and freshwater usage. Soilless agriculture has emerged as a more sustainable practice to mitigate this issue but still generates nutrient-rich drainwater that can harm the environment if not properly managed. In this context, this study explores a circular economy approach to reuse the drainwa...


Tailored bacterial co-cultures improve Tisochrysis lutea growth and nutrient pr...

Santos, Tamara; da Fonseca Simões, Beatriz; Rossetto, Veronica; Pereira, Hugo; Maia, Inês Beatriz; Oliveira, Marta; Esteves Lopes Navalho, João Carlos

The marine haptophyte Tisochrysis lutea is a valuable source of high-value compounds, including polyunsaturated fatty acids like docosahexaenoic acid, and pigments (e.g., fucoxanthin). However, high production costs and variability remain major challenges for its large-scale application in aquaculture, pharmaceuticals, and biotechnology industries. Therefore, strategies to enhance biomass production and quality...


Transcriptomic insights into metabolic reprogramming and exopolysaccharide synt...

GUERREIRO, Maurean; Emmanuel, Coline; Dupuits, Céline; Gardarin, Christine; Mouzeyar, Said; Varela, João; Roche, Jane; laroche, céline

Porphyridium species are known red microalgae for producing valuable bioactive compounds such as sulfated exopolysaccharides (EPS) with diverse industrial biomedical applications due to their functional and rheological properties. Recent studies have investigated how abiotic stresses, particularly nitrogen deprivation, affect Porphyridium’s metabolic regulation and EPS production through transcriptomic analysis...


Yeast species associated with industrial cultures of the marine microalgae tiso...

Matos, Madalena; Fernandes, Mónica A.; Coelho, Natacha; Santos, Tamara; Varela, João; Rodrigues, Alexandre M. C.; Sá-Correia, Isabel

This study provides the first systematic characterization of culturable yeast diversity associated with large-scale cultivation of Tisochrysis lutea. This marine haptophyte is widely used in aquaculture for its high content of essential fatty acids, pigments, and other bioactive compounds. Culture sampling was conducted at Necton S.A. facilities (Olhão, Portugal) over full production cycles from 5 L flasks unti...


Enhancing growth, nutrient uptake and flowering of Tagetes patula plants throug...

Vieira, João; Saque, Moisés; Viana, Catarina; Rodrigues, Maria Helena; Guerreiro David Coelho, Luísa Isabel; Gama, Florinda; Varela, João; Pereira, Hugo

Tagetes patula L. is a prominent plant that is frequently used for its ornamental value. Marigolds are sources of various compounds of great interest to agricultural, pharmaceutical, and nutritional industries. Microalgae can be applied as a biostimulant as they have multifunctional properties in agriculture, namely facilitating nutrient uptake, improving crop performance and physiological status, and thus incr...


Impact of seasons on industrial cultivation of Limnospira platensis (Spirulina)...

Guerra, Inês; Torres, Cátia; Cardoso, Helena; Pereira, Hugo; Trovão, Mafalda; Rodrigues, Alexandre M. C.; Varela, João

Spirulina is the most commercially produced biomass, among microalgae and cyanobacteria, with extensive applications across food, feed, nutraceutical and biotechnological sectors. While Spirulina cultivation is wellcharacterized at laboratorial and small-scale production, critical knowledge gaps remain regarding industrialscale production in temperate climates. Specifically, there is a lack of robust empirical ...


Heterotrophic cultivation of Chlorella vulgaris yellow mutant on sidestreams: M...

Trovão, Mafalda; Barros, Ana; Machado, Adriana; Reis, Ana; Pedroso, Humberto; Espírito Santo, Gonçalo; Correia, Nádia; Costa, Monya; Ferreira, Sara

Microalgal protein is a promising feedstock to complement and/or replace other protein sources. Besides requiring less land and water usage, microalgae production is a more sustainable process, especially if industrial sidestreams are used as nutrient sources. Additionally, the heterotrophic cultivation of microalgae, such as Chlorella vulgaris, enables the achievement of much higher biomass productivity and lo...


Exopolysaccharides from porphyridium cruentum as a reinforcing agent for chitos...

Lazzarotto Cristofoli, Nathana; Ribeiro Lima, Alexandre; Costa, Sebastião; Silva, Carlos Manuel; Varela, João; Vieira, Margarida

Microalgae exopolysaccharides (EPS), extracted from exhausted culture medium of Porphyridium cruentum, a rhodophyte able to grow in marine environments, may be useful as reinforcing agents in chitosan-based films produced by casting, using glycerol as a plasticizer. EPS were added at 1 and 2 % (w/v). The resulting hydrophilic films, EPS1 and EPS2, respectively, were opaquer than the control (CTR) chitosan-based...


Nannochloropsis lipids and polyunsaturated fatty acids: potential applications ...

Navalho, Sofia; Narcis Ferrer-Ledo; Maria J. Barbosa; Varela, João

The genus Nannochloropsis comprises a group of oleaginous microalgae that accumulate polyunsaturated fatty acids (PUFAs), especially eicosapentaenoic acid (EPA). These molecules are essential for the correct development and health of humans and animals. Thanks to their attractive lipid profile, Nannochloropsis is mainly marketed as a feed ingredient in aquaculture. In microalgae of this genus, contents and cell...


Comparison of different microalgae biomass typologies used in rotifers enrichme...

de Castro, Daniela; Castaldi, Matthew; Martins, Gil; Santos, Tamara; Pereira, Hugo; Diogo, Patrícia; Varela, João; Gavaia, Paulo

The use of enriched rotifers with industrially produced microalgae represents a valuable tool for the enhancement of zebrafishlarval nutrition and increased biological performance. Currently, a monoculture of microalgal species (Nannochloropsis sp.) inform of liquid paste is routinely used for rotifers enrichment for zebrafish larvae feeding; however, the most adequate typology (i.e.,paste or freeze-dried) of the...


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