10 documents found, page 1 of 1
Insights into the full-length SRPK2 structure and its hydrodynamic behavior
Alves Barbosa, Everton de Almeida; Seraphim, Thiago Vargas; Gandin, Cesar Augusto [UNESP]; Teixeira, Leilane FerreiraMade available in DSpace on 2019-10-04T12:42:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-09-15; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Fundacao Arthur Bernardes (FUNARBE ...
Label-free assay based on immobilized capillary enzyme reactor of Leishmania in...
Vasconcellos, Raphael de Souza; Magalhães, Luana; Oliveira, Arthur Henrique Cavalcante de; Mariotini-Moura, Christiane; Firmino, Rafaela de CássiaNucleoside triphosphate diphosphohydrolase (NTPDase) is an enzyme belonging to the apyrase family that participates in the hydrolysis of the nucleosides di- and triphosphate to the corresponding nucleoside monophosphate. This enzyme underlies the virulence of parasites such as Leishmania. Recently, an NTPDase from Leishmania infantum (LicNTPDase-2) was cloned and expressed and has been considered as a new drug ...
Trypanosoma cruzi nucleoside triphosphate diphosphohydrolase 1 (TcNTPDase-1) bi...
Mariotini-Moura, Christiane; Bastos, Matheus Silva e; Castro, Felipe Freitas de; Trindade, Mellina Lanna; Vasconcellos, Raphael de SouzaPrevious work has suggested that Trypanosoma cruzi diphosphohydrolase 1 (TcNTPDase-1) may be involved in the infection of mammalian cells and serve as a potential target for rational drug design. In this work, we produced recombinant TcNTPDase-1 and evaluated its nucleotidase activity, cellular localization and role in parasite adhesion to mammalian host cells. TcNTPDase-1 was able to utilize a broad range of t...
The Antileishmanial Potential of C-3 Functionalized Isobenzofuranones against L...
Pereira, Wagner Luiz; Vasconcellos, Raphael de Souza; Mariotini-Moura, Christiane; Gomes, Rodrigo Saar; Firmino, Rafaela de CássiaLeishmaniases are diseases caused by protozoan parasites of the genus Leishmania. Clinically, leishmaniases range from cutaneous to visceral forms, with estimated global incidences of 1.2 and 0.4 million cases per year, respectively. The treatment of these diseases relies on multiple parenteral injections with pentavalent antimonials or amphotericin B. However, these pharmaceuticals are either too toxic or expe...
Recombinant Leishmania (Leishmania) infantum Ecto-Nucleoside Triphosphate Dipho...
Souza, Ronny Francisco de; Santos, Yaro Luciolo dos; Vasconcellos, Raphael de Souza; Borges-Pereira, Lucas; Almeida, Márcia Rogéria deCanine visceral leishmaniasis is an important public health concern. In the epidemiological context of human visceral leishmaniasis, dogs are considered the main reservoir of Leishmania parasites; therefore, dogs must be epidemiologically monitored constantly in endemic areas. Furthermore, dog to human transmission has been correlated with emerging urbanization and increasing rates of leishmaniasis infection wo...
E-NTPDase (ecto-nucleoside triphosphate diphosphohydrolase) of Leishmania amazo...
Gomes, Rodrigo Saar; Carvalho, Luana Cristina Faria; Vasconcellos, Raphael de Souza; Fietto, Juliana Lopes Rangel; Afonso, Luís Carlos CroccoLeishmania amazonensis, the causal agent of diffuse cutaneous leishmaniasis, is known for its ability to modulate the host immune response. Because a relationship between ectonucleotidase activity and the ability of Leishmania to generate injury in C57BL/6 mice has been demonstrated, in this study we evaluated the involvement of ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) activity of L. amazonen...
Expressão heteróloga e caracterização bioquímica da nucleosídeo trifosfato difo...
Vasconcellos, Raphael de SouzaVisceral leishmaniasis is a severe disease, responsible for about 57.000 deaths per year all over the world, having Leishmania chagasi as the etiological agent in the New World. Protozoa of the genus Leishmania belong to the Tripanosomatidae family, wich is incapable of synthetize the purine ring. It is believed that the nucleoside triphosphate diphosphohydrolases (NTPDases) might play a role in purine salvage ...
Estudo bioquímico, biológico e busca por inibidores da nucleosídeo trifosfato d...
Vasconcellos, Raphael de SouzaVisceral leishmaniasis is a serious disease, responsible for about 57000 deaths per year worldwide. Parasites of the genus Leishmania are unable to synthesize purines, and it is believed that the nucleotide triphosphate diphosphohydrolases (NTPDases) are involved in this process, and even in the immune system by hydrolysis of extracellular nucleotides. In this work, the Leishmania infantum chagasi NTPDase-2 (Li...
Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate dipho...
Souza, Ronny Francisco de; Santos, Yaro Luciolo dos; Vasconcellos, Raphael de Souza; Pereira, Lucas Borges; Caldas, Ivo SantanaCanine visceral leishmaniasis is an important public health concern. In the epidemiological context of human visceral leishmaniasis, dogs are considered the main reservoir of Leishmania parasites; therefore, dogs must be epidemiologically monitored constantly in endemic areas. Furthermore, dog to human transmission has been correlated with emerging urbanization and increasing rates of leishmaniasis infection wo...
Trypanosoma cruzi nucleoside triphosphate diphosphohydrolase 1 (TcNTPDase-1) bi...
Moura, Christiane Mariotini; Bastos, Matheus Silva e; Castro, Felipe Freitas de; Trindade, Mellina Lanna; Vasconcellos, Raphael de SouzaPrevious work has suggested that Trypanosoma cruzi diphosphohydrolase 1 (TcNTPDase-1) may be involved in the infection of mammalian cells and serve as a potential target for rational drug design. In this work, we produced recombinant TcNTPDase-1 and evaluated its nucleotidase activity, cellular localiza- tion and role in parasite adhesion to mammalian host cells. TcNTPDase-1 was able to utilize a broad range of...