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Purification, biochemical characterization, and amino acid sequence of a novel ...
Carneiro, Rômulo Farias; Torres, Renato Cézar Farias; Chaves, Renata Pinheiro; Vasconcelos, Mayron Alves de; Sousa, Bruno Lopes deA new lectin from Aplysia dactylomela eggs (ADEL) was isolated by affinity chromatography on HClactivated Sepharose™ media. Hemagglutination caused by ADEL was inhibited by several galactosides, mainly galacturonic acid (Ka = 6.05 × 106 M−1 ). The primary structure of ADEL consists of 217 residues, including 11 halfcystines involved in five intrachain and one interchain disulfide bond, resulting in a molecular ...
Purification, partial characterization and immobilization of a mannose-specific...
Pinto Júnior, Vanir Reis; Santiago, Mayara Queiroz de; Osterne, Vinícius José da Silva; Correia, Jorge Luis AlmeidaLectin from the seeds of Dioclea lasiophylla (DlyL) was purified in a single step by affinity chromatography on a Sephadex® G-50 column. DlyL strongly agglutinated rabbit erythrocytes and was inhibited by monosaccharides (D-mannose and α-methyl-Dmannoside) and glycoproteins (ovalbumin and fetuin). Similar to other Diocleinae lectins, DlyL has three chains, α, β and γ, with mass of 25,569 ± 2, 12,998 ± 1 and 12,...
A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partia...
Alves, Ana Cecília; Vasconcelos, Mayron Alves de; Santiago, Mayara Quiroz; Pinto-Junior, Vanir Reis; Osterne, Vinicius José SilvaA novel lectin from seeds of Clathrotropis nitida (CNA) was purified and characterized. CNA is a glycoprotein containing approximately 3.3% carbohydrates in its structure. CNA promoted intense agglutination of rabbit erythrocytes, which was inhibited by galactosides and porcine stomach mucin (PSM). The lectin maintained its hemagglutinating activity after incubation in a wide range of temperatures (30-60 °C) an...
Atividade antimicrobiana e antibiofilme de extratos hexânicos de croton blanche...
Pereira, Rafael Nascimento; Pereira, Anna Luísa; Vasconcelos, Mayron Alves de; Arruda, Francisco Vassiliepe Sousa; Silveira, Edilberto RochaOs vegetais são conhecidos por produzir grande variedade de compostos ativos que atualmente têm se tornando importantes na busca de novos fármacos. De fato, extratos vegetais tem demonstrado efeito antimicrobiano, atuando sobre formas planctônicas e inibindo a formação de biofilmes. Diante do exposto, o objetivo deste trabalho foi avaliar o efeito antimicrobiano e antibiofilme de extratos hexânicos de diferente...
Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microb...
Vasconcelos, Mayron Alves deVASCONCELOS, Mayron Alves de. Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico. 2013. 266 f. Tese (Doutorado em bioquímica)- Universidade Federal do Ceará, Fortaleza-CE, 2013.; Biofilms are microbial communities that are irreversibly attached to a surface and are embedded in a polymeric matrix produced by them. Biofilms are commonly related to nosocomial ...
Atividade das lectinas de Canavalia brasiliensis e Canavalia ensiformis sobre o...
Vasconcelos, Mayron Alves deVASCONCELOS, M. A. Atividade das lectinas de Canavalia brasiliensis e Canavalia ensiformis sobre o crescimento “in vitro” de Rhizobium tropici. 2010. 87f. Dissertação (Mestrado em Biotecnologia) - Campus de Sobral, Universidade Federal do Ceará, Sobral, 2010.; Rizobia are gram-negative bacteria that lives as symbiotic cells in roots of leguminous inducing the formation of nodules. Inside of these nodules, these...
Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microb...
Vasconcelos, Mayron Alves deBiofilms are microbial communities that are irreversibly attached to a surface and are embedded in a polymeric matrix produced by them. Biofilms are commonly related to nosocomial infections that showing an enhanced resistance to antimicrobial agents compared to planktonic cells, thus hindering their treatments and limiting therapeutic options. In this context, the search for new molecules with antimicrobial an...