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Gastrointestinal delivery of codfish skin-derived collagen hydrolysates: deep e...
Silva, Isa; Vaz, Bárbara M. C.; Sousa, Sérgio; Pintado, Maria Manuela; Coscueta, Ezequiel R.; Ventura, Sónia P. M.The fishing industry produces substantial by-products, such as heads, skins, bones, and scales, rich in collagen—a prevalent protein in these materials. However, further application of deep eutectic solvent-based extraction remains unexplored. In this study, we extracted collagen with urea: propanoic acid mixture (U:PA; 1:2) with a 2.2% yield, followed by enzymatic hydrolysis with alcalase for 120 min. The resu...
Unlocking the full potential of green propolis: a novel extraction approach usi...
Contieri, Letícia S.; Ribeiro, Tânia B.; Sosa, Filipe H. B.; Vaz, Bárbara M. C.; Pizani, Rodrigo S.; Pintado, Manuela; Ventura, Sónia P. M.Propolis is highly diverse and has various health benefits for humans. However, standardizing propolis-based products for use in the pharmaceutical and food industries is challenging. Efficient extraction processes are essential to maximize their biological potential. Ethanol is commonly used as a solvent but has several drawbacks, such as residual flavor and limitations for some individuals. Water is an altern...
Recovery of bacterioruberin and proteins using aqueous solutions of surface-act...
Vaz, Bárbara M. C.; Kholany, Mariam; Pinto, Diana C. G. A.; Macário, Inês P. E.; Veloso, Telma; Caetano, Tânia; Pereira, Joana L.; Coutinho, João A. P.Haloarchaea microorganisms are little explored marine resources that can be a promising source of valuable compounds with unique characteristics, due to their adaptation to extreme environments. In this work, the extraction of bacterioruberin and proteins from Haloferax mediterranei ATCC 33500 was investigated using aqueous solutions of ionic liquids and surfactants, which were further compared with ethanol. De...
Using aqueous solutions of ionic liquids as chlorophyll eluents in solid-phase ...
Vaz, Bárbara M. C.; Martins, Margarida; de Souza Mesquita, Leonardo M.; Neves, Márcia C.; Fernandes, Andreia P. M.; Pinto, Diana C. G. A.There is a need for handy and fast techniques to purify biomolecules, increasing their stability and value, because the separation units are current bottlenecks in downstream processes. Solid-phase extraction is a technique that enables the purification of a compound by its adsorption from a liquid matrix. The AmberLite™ HPR900 OH resin allows the separation of chlorophylls from complex extracts, however the re...