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Treating domestic wastewater towards freshwater quality: bacterial community an...

Leão, Inês; Antunes, Jorge; Baptista, Inês; Jorge, Ruben; Marinheiro, Luís; Löblich, Stefan; Vaz-Moreira, Ivone; Manaia, Célia M.

Ideally, wastewater treatment aims to produce water indistinguishable from freshwater, especially for reuse. This study evaluated bacterial community and antibiotic resistance variations throughout treatment and benchmarked these with freshwater sources. Samples collected from six points of a full-scale wastewater treatment plant, pilot-scale advanced treatment options (non-thermal plasma - NTP, ultrafiltration...


Impact of red wine vinegar-based solution on bacterial communities of squid and...

Rezende, Lourenço Pinto de; Barbosa, Joana Bastos; Carvalho, Teresa Bento de; Vaz-Moreira, Ivone; Monteiro, Maria João P.; Teixeira, Paula

Seafood is an essential component of a balanced and healthy diet, which increases its demand. However, its biological composition and high moisture content make these products extremely perishable. To prevent spoilage, and the consequent food waste and fnancial expenses throughout the seafood supply chain, new technologies have been successfully developed to inhibit bacterial growth, the main cause of seafood s...


Bacterial population variability in wastewater treatment and the potential pers...

Manaia, Célia; Ribeiro, Diana; Ruivo, Afonso; Santos, Isabel; Mena, Cristina; Vaz-Moreira, Ivone; Rosa, Maria João; Campinas, Margarida; Mesquita, Elsa

Wastewater treatment plants (WWTPs) are essential barriers to attenuate the emission of contaminants, such as antibiotic-resistant bacteria (ARB) and resistance genes (ARGs), which monitoring is becoming a requirement (Directive (EU) 2024/3019). Despite the urgent need to improve wastewater treatment to remove ARB&ARGs, the complexity of microbial community dynamics during treatment remains poorly understood. T...


Bacteriological safety and quality of composted products from animal, urban or ...

Vaz-Moreira, Ivone; D’Arnese, Angelo; Knoll, Maurice; Teixeira, A. Margarida; Barbosa, Joana Bastos; Teixeira, Paula; Manaia, Célia M.

Abstract: This study investigated the presence of culturable bacterial pathogens, and antibiotic resistance and associated genes (quantitative PCR) in commercially available composted products from animal excrements or manure (n=7), urban wastes (n=1) or (sewage sludge) (n=1). Metals quantification and 16S rRNA-based bacterial community composition analyses supported the results to infer potential risks to down...


Dynamics and interrelationships between antibiotic resistance, organic micropol...

Teixeira, A. Margarida; Matos, Diana; Coelho, Norberta; Halwatura, Lahiruni M.; Vaz-Moreira, Ivone; Castro, Paula M. L.; Aga, Diana S.; Manaia, Célia M.

Constructed wetlands systems (CWs) are increasingly regarded as promising alternatives or complements to conventional wastewater treatment processes. However, the fate of chemical and biological contaminants in realworld treatment processes is understudied in this type of systems. This study aimed to fill this gap by evaluating the response of three horizontal subsurface flow CWs, in Northern Portugal, planted ...


Antimicrobial resistance in urban wastewater: insights from operating condition...

Ribeiro, Diana; Ruivo, Afonso; Santos, Isabel; Mena, Cristina; Vaz-Moreira, Ivone; Costa, Cláudio; Faria, Gabriela; Campinas, Margarida; Mesquita, Elsa


Towards the definition of an antibiotic resistome signature in wastewater and d...

Cachetas, Diogo Macedo; Vaz-Moreira, Ivone; Pereira, Vítor; Manaia, Célia M.

Domestic wastewater is a significant reservoir of antibiotic resistance genes, which pose environmental and public health risks. We aimed to define an antibiotic resistome signature, represented by core genes, i.e., shared by 90% of the metagenomes of each of three conceptual environmental compartments wastewater (influent, sludge, effluent), freshwater, and agricultural soil. The definition of resistome signat...


Towards the definition of an antibiotic resistome signature in wastewater and d...

Cachetas, Diogo; Vaz-Moreira, Ivone; Pereira, Vítor; Manaia, Célia M.

Domestic wastewater is a significant reservoir of antibiotic resistance genes, which pose environmental and public health risks. We aimed to define an antibiotic resistome signature, represented by core genes, i.e., shared by ≥ 90% of the metagenomes of each of three conceptual environmental compartments – wastewater (influent, sludge, effluent), freshwater, and agricultural soil. The definition of resistome si...


Fecal contamination, antibiotic resistance and organic micropollutants in full-...

Teixeira, Ana Margarida Ribeiro; Matos, Diana; Coelho, Norberta; Halwatura, Lahiruni M.; Abaya, Liezel Mari; Vaz-Moreira, Ivone; Castro, Paula M. L.

Background and Aims Constructed wetlands (CWs) are recognized as a promising alternative or complement to traditional wastewater treatments. Their cost-effectiveness and seamless integration into natural landscapes, fostering wildlife habitats, are notable advantages. This study aimed to assess the efficacy of three horizontal subsurface flow CWs with Phragmites australis for reducing fecal contamination, antib...


Resistência a antibióticos e micropoluentes orgânicos em três sistemas de zona ...

Teixeira, A. Margarida; Matos, Diana; Halwatura, Lahiruni M.; Vaz-Moreira, Ivone; Castro, Paula M. L.; Aga, Diana S.; Manaia, Célia M.

As zonas húmidas construídas, também conhecidas como fito-estações de tratamento de águas residuais (fito-ETAR), têm vindo a ser consideradas promissoras alternativas ou complementos para os processos convencionais de tratamento de águas residuais. A relação custo-benefício e a integração na paisagem natural, com a criação de habitats para a vida selvagem, são vantagens importantes destas soluções naturais. Est...


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