8 documents found, page 1 of 1

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Semi-automated sequence curation for reliable reference datasets in ITS2 vascul...

Quaresma, Andreia; Ankenbrand, Markus J.; Garcia, Carlos Ariel Yadró; Rufino, José; Honrado, Mónica; Amaral, Joana S.; Brodschneider, Robert

One of the most critical steps for accurate taxonomic identification in DNA (meta)-barcoding is to have an accurate DNA reference sequence dataset for the marker of choice. Therefore, developing such a dataset has been a long-term ambition, especially in the Viridiplantae kingdom. Typically, reference datasets are constructed with sequences downloaded from general public databases, which can carry taxonomic and...

Date: 2024   |   Origin: Biblioteca Digital do IPB

Bio-Monitoring of environmental pollution using the citizen science approach

Steen, Jozef van der; Amaral, Joana S.; Baveco, Hans; Blanco Muñoz, Patricia; Brodschneider, Robert; Brusbardis, Valters; Buddendorf, Bas

Honeybee colonies are excellent bio-samplers of biological material such as nectar, pollen, and plant pathogens, as well as non-biological material such as pesticides or airborne contamination. The INSIGNIA-EU project aims to design and test an innovative, non-invasive, scientifically proven citizen science environmental monitoring protocol for the detection of pesticides, microplastics, heavy metals, and air p...

Date: 2022   |   Origin: Biblioteca Digital do IPB

Honey bee collected pollen for botanical identification via its2 metabarcoding:...

Quaresma, Andreia; Brodschneider, Robert; Gratzer, Kristina; Gray, Alison; Keller, Alexander; Kilpinen, Ole; Rufino, José; Steen, Jozef van der

While classical palynology has been the method of choice to assess botanical diversity of bee-collected pollen for multiple purposes, DNA metabarcoding is emerging as a powerful alternative being able to achieve high taxonomic identification accuracy. Moreover,DNA metabarcoding allows analysis of hundreds of samples in a single high-throughput sequencing run, therefore offering unprecedented scale in citizen sc...

Date: 2022   |   Origin: Biblioteca Digital do IPB

Honey bee collected pollen for botanical identification via its2 metabarcoding:...

Quaresma, Andreia; Brodschneider, Robert; Gratzer, Kristina; Gray, Alison; Keller, Alexander; Kilpinen, Ole; Rufino, José; Steen, Jozef van der

DNA metabarcoding is emerging as a powerful method for botanical identification of bee-collected pollen, allowing analysis of hundreds of samples in a single high-throughput sequencing run, therefore offering unprecedented scale in citizen science projects. Biases in metabarcoding can be introduced at any stage of sample processing and preservation is the first step of the pipeline. Hence, it is important to te...

Date: 2022   |   Origin: Biblioteca Digital do IPB

Preservation methods of honey bee-collected pollen are not a source of bias in ...

Quaresma, Andreia; Brodschneider, Robert; Gratzer, Kristina; Gray, Alison; Keller, Alexander; Kilpinen, Ole; Rufino, José; Steen, Jozef van der

Pollen metabarcoding is emerging as a powerful tool for ecological research and offers unprecedented scale in citizen science projects for environmental monitoring via honey bees. Biases in metabarcoding can be introduced at any stage of sample processing and preservation is at the forefront of the pipeline. While in metabarcoding studies pollen has been preserved at − 20 °C (FRZ), this is not the best method f...

Date: 2021   |   Origin: Biblioteca Digital do IPB

Introducing the INSIGNIA project: Environmental monitoring of pesticides use th...

Carreck, Norman L.; Amaral, Joana S.; Anagnostopoulos, Christ; Baveco, Hans; Bieszczad, Sarah; Biron, David G.; Brodschneider, Robert

INSIGNIA aims to design and test an innovative, non-invasive, scientifically proven citizen science environmental monitoring protocol for the detection of pesticides via honey bees. It is a pilot project initiated and financed by the European Commission (PP-1-1-2018; EC SANTE). The study is being carried out by a consortium of specialists in honey bees, apiculture, chemistry, molecular biology, statistics, anal...

Date: 2019   |   Origin: Biblioteca Digital do IPB

Introducing the INSIGNIA project: environmental monitoring of pesticide use thr...

Steen, Jozef van der; Brodschneider, Robert; Gratzer, Kristina; Bieszczad, Sarah; Hatjina, Fani; Charistos, Leonidas; Carreck, Norman L.; Gray, Alison

INSIGNIA aims to design and test an innovative, non-invasive, scientifically proven citizen science environmental monitoring protocol for the detection of pesticides by honey bees. It is a 30-month pilot project initiated and financed by the EC (PP-1-1-2018; EC SANTE). The study is being carried out by a consortium of specialists in honey bees, apiculture, statistics, analytics, modelling, extension, social sci...

Date: 2019   |   Origin: Biblioteca Digital do IPB

INSIGNIA: um projeto de monitorização ambiental de pesticidas através da utiliz...

Pinto, M. Alice; Amaral, Joana S.; Baveco, Hans; Biron, David G.; Brodschneider, Robert; Brusbardis, Valters; Carreck, Norman L.; Charistos, Leonidas

INSIGNIA ("cItizeN Science InvestiGatioN for pestIcIcides in Apicultutarl products"; https://www.insignia-bee,eu/) é um projecto financiado pela agência "Directorate General for Health and Food Safety" da Comissão Europeia, e que teve início em Outubro de 2018. O consórcio INSIGNIA é coordenado por Jozef van der Steen e integra 16 instituições parceiras de 12 países Europeus, entre as quais está o Centro de Inv...

Date: 2019   |   Origin: Biblioteca Digital do IPB

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