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Inclusion of pineapple by-products as natural antioxidant sources in diets for ...

Pereira, Ricardo; Basto, Ana; Pintado, Manuela; Valente, Luisa M. P.; Velasco, Cristina

This study investigates the effects of pineapple by-products on feed preservation during storage at two different temperatures (25 °C and 4 °C) and on European sea bass (Dicentrarchus labrax) stress resistance. Four isoproteic, isolipidic, and isoenergetic diets were manufactured: CTRL—negative control, commercial diet without added antioxidants; VITE—positive control, CTRL diet with 100 mg kg−1 of vitamin E; a...


Locally-sourced animal protein hydrolysates in high-plant-protein diets can pro...

Rodrigues-dos-Santos, Luciano; Basto, Ana; Monteiro, Marta; António, Carla; Rodrigues, Ana M.; Sá, Tiago; Velasco, Cristina; Martins, Rui; Rosa, Ana

Upcycling meat and fish by-products into bioactive protein hydrolysates promotes zero-waste practices within the circular bioeconomy and provides locally-sourced alternatives to replace fishmeal (FM) in aquafeeds. In this study, novel hydrolysates were developed from blue shark skin, fish by-products, and swine processed animal protein, aiming to reduce the high-quality FM inclusion in European seabass (Dicentr...


Unravelling the effects of extrusion and drying temperatures on the radical sca...

Pereira, Ricardo; Velasco, Cristina; Gómez-Garcia, Ricardo; Dias, Jorge; Pintado, Manuela; Valente, Luisa M. P.

Antioxidant supplementation of aquafeeds with natural sources is a current research trend within the context of a circular economy. However, natural antioxidants are highly vulnerable to thermal conditions during feed manufacturing, particularly during extrusion and drying. This study examines the impact of extrusion and drying temperatures on the antioxidant properties of aquafeeds supplemented with natural an...


Incorporating sardine cooking water aromas into plant-based diets for european ...

Resende, Daniela; Velasco, Cristina; Pereira, Maria J.; Sá, Tiago; Rocha, Célia; Cunha, Luís M.; Lima, Rui C.; Brazinha, Carla; Pintado, Manuela

Sardine cooking wastewaters are by-products of the canning industry with great potential for valorisation. We have hypothesized that they can be a source of aromas to enhance appetite when added to plant-based diets. The poor palatability of such diets often described in carnivorous species poses a recurring problem in fish farming, with harsh consequences on fish growth performance and flesh quality. Aromas fr...


Production of aroma-rich extracts from sardine cooking wastewaters: exploring t...

Resende, Daniela; Pereira, Maria J.; Sá, Tiago; Brazinha, Carla; Pintado, Manuela; Valente, Luisa M. P.; Velasco, Cristina

Managing the canning industry’s nutrient-rich and odorous liquid waste is a hurdle. Concurrently, the growing use of vegetable ingredients in aquafeeds diminishes palatability and feed consumption in carnivorous fish. Thus, we hypothesized that aromas could be extracted from cooking wastewaters at canning factories and added to plant-based diets to stimulate intake in European seabass. Sardine cooking wastewate...


Repurposing sardine cooking wastewaters as ingredients for European seabass diets

Resende, Daniela; Velasco, Cristina; Pereira, Maria J.; Sá, Tiago; Rocha, Célia; Cunha, Luís M.; Lima, Rui C.; Brazinha, Carla; Pintado, Manuela


Stress response of European seabass (Dicentrarchus labrax) fed plant-based diet...

Resende, Daniela; Pereira, Ricardo; Domínguez, David; Pereira, Miguel; Pereira, Carlos; Pintado, Manuela; Valente, Luísa M. P.; Velasco, Cristina

To improve fish welfare, it is essential that aquafeeds are designed to help fish cope with the stressful conditions of fish farms. One effective strategy to achieve this goal is to supplement the diet with bioactive hydrolysates. Here, diet supplementation to modulate oxidative stress after air exposure was investigated in European seabass, using swine blood hydrolysates (BH), obtained either by autohydrolysis...


Exploring the influence of sardine cooking water extracts on the short-term reg...

Resende, Daniela; Pereira, Maria J.; Sá, Tiago; Brazinha, Carla; Pintado, Manuela; Valente, Luísa M. P.; Velasco, Cristina

Introduction In fish, feed intake regulation is mediated by an interaction between homeostatic and hedonic signals (Soengas et al., 2018). The homeostatic control relates to biological needs regarding nutrients and energy (Soengas, 2021). On the other hand, the hedonic control is associated with reward-based routes, which are triggered by highly palatable food (Díaz-Rúa et al., 2022). Hedonic control may overri...


Dietary inclusion of blood hydrolysates affects muscle growth in European seaba...

Velasco, Cristina; Resende, Daniela; Oliveira, Beatriz; Canada, Paula; Pereira, Miguel; Pereira, Carlos; Pintado, Manuela; Valente, Luisa M. P.

Dietary supplementation with hydrolysates has been suggested to influence muscle protein synthesis and fish growth. This study assessed the impact of including 3% swine blood hydrolysates (BH) in a plant-based diet on muscle cellularity and the expression of molecular markers related to muscle fibre proliferation and hypertrophic growth of European seabass. Three BH fractions were obtained by two different proc...


Dietary inclusion of sardine cooking waters: impact on appetite regulation, gro...

Resende, Daniela; Velasco, Cristina; Pereira, Maria J.; Sá, Tiago; Rocha, Célia; Cunha, Luís M.; Lima, Rui C.; Brazinha, Carla; Pintado, Manuela

Introduction Plant-based ingredients have been considered suitable options for replacing marinebased ones in aquafeeds (Naylor et al., 2021), but this may compromise diet palatability (Geurden et al., 2013), potentially affecting fish growth and flesh quality. To overcome this, feed attractants have been suggested. These compounds, like alcohols, aldehydes, and organic acids, were reported to stimulate intake i...


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