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Density separation of petrous bone powders for optimized ancient DNA yields

Fernandes, Daniel; Sirak, Kendra A.; Cheronet, Olivia; Novak, Mario; Brück, Florian; Zelger, Evelyn; Llanos-Lizcano, Alejandro; Wagner, Anna

Density separation is a process routinely used to segregate minerals, organic matter, and even microplastics, from soils and sediments. Here we apply density separation to archaeological bone powders before DNA extraction to increase endogenous DNA recovery relative to a standard control extraction of the same powders. Using nontoxic heavy liquid solutions, we separated powders from the petrous bones of 10 indi...


An integrative skeletal and paleogenomic analysis of stature variation suggests...

Marciniak, Stephanie; Bergey, Christina M.; Silva, Ana Maria; Hałuszko, Agata; Furmanek, Mirosław; Veselka, Barbara; Velemínský, Petr

Human culture, biology, and health were shaped dramatically by the onset of agriculture ∼12,000 y B.P. This shift is hypothesized to have resulted in increased individual fitness and population growth as evidenced by archaeological and population genomic data alongside a decline in physiological health as inferred from skeletal remains. Here, we consider osteological and ancient DNA data from the same prehistor...


The Beaker phenomenon and the genomic transformation of northwest Europe

Olalde, Iñigo; Brace, Selina; Allentoft, Morten E; Armit, Ian; Kristiansen, Kristian; Booth, Thomas; Rohland, Nadin; Mallick, Swapan

From around 2750 to 2500 bc, Bell Beaker pottery became widespread across western and central Europe, before it disappeared between 2200 and 1800 bc. The forces that propelled its expansion are a matter of long-standing debate, and there is support for both cultural diffusion and migration having a role in this process. Here we present genome-wide data from 400 Neolithic, Copper Age and Bronze Age Europeans, in...


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